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Q60034

- DYR_THEMA

UniProt

Q60034 - DYR_THEMA

Protein

Dihydrofolate reductase

Gene

folA

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.By similarity

    Catalytic activityi

    5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

    Temperature dependencei

    Highly thermostable.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei7 – 71Substrate; via carbonyl oxygen
    Binding sitei9 – 91NADP; via amide nitrogen and carbonyl oxygen1 Publication
    Binding sitei16 – 161NADP; via carbonyl oxygen1 Publication
    Binding sitei28 – 281Substrate
    Binding sitei59 – 591SubstrateBy similarity
    Binding sitei81 – 811NADP; via carbonyl oxygen1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi46 – 516NADP1 Publication
    Nucleotide bindingi65 – 673NADP1 Publication
    Nucleotide bindingi103 – 1053NADP1 Publication

    GO - Molecular functioni

    1. dihydrofolate reductase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. glycine biosynthetic process Source: InterPro
    2. nucleotide biosynthetic process Source: InterPro
    3. one-carbon metabolic process Source: UniProtKB-KW
    4. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-461.
    TMAR243274:GC6P-1687-MONOMER.
    UniPathwayiUPA00077; UER00158.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrofolate reductase (EC:1.5.1.3)
    Short name:
    DHFR
    Gene namesi
    Name:folA
    Synonyms:dyrA
    Ordered Locus Names:TM_1641
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 169169Dihydrofolate reductasePRO_0000186418Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi243274.TM1641.

    Structurei

    Secondary structure

    1
    169
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 108
    Beta strandi15 – 173
    Helixi26 – 3914
    Beta strandi41 – 455
    Helixi46 – 527
    Beta strandi59 – 646
    Beta strandi76 – 794
    Helixi83 – 9210
    Beta strandi96 – 1027
    Helixi104 – 1129
    Beta strandi117 – 1237
    Beta strandi125 – 1306
    Beta strandi132 – 1354
    Beta strandi141 – 15111
    Beta strandi153 – 1553
    Beta strandi158 – 1647

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CZ3X-ray2.10A/B2-169[»]
    1D1GX-ray2.10A/B2-169[»]
    ProteinModelPortaliQ60034.
    SMRiQ60034. Positions 2-169.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ60034.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 169167DHFRPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the dihydrofolate reductase family.Curated
    Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0262.
    KOiK00287.
    OMAiKGYERVA.
    OrthoDBiEOG6KT2V2.

    Family and domain databases

    Gene3Di3.40.430.10. 1 hit.
    InterProiIPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR001796. DHFR_dom.
    [Graphical view]
    PfamiPF00186. DHFR_1. 1 hit.
    [Graphical view]
    PRINTSiPR00070. DHFR.
    SUPFAMiSSF53597. SSF53597. 1 hit.
    PROSITEiPS51330. DHFR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60034-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKVIFVLAM DVSGKIASSV ESWSSFEDRK NFRKITTEIG NVVMGRITFE    50
    EIGRPLPERL NVVLTRRPKT SNNPSLVFFN GSPADVVKFL EGKGYERVAV 100
    IGGKTVFTEF LREKLVDELF VTVEPYVFGK GIPFFDEFEG YFPLKLLEMR 150
    RLNERGTLFL KYSVEKSHR 169
    Length:169
    Mass (Da):19,367
    Last modified:November 1, 1997 - v1
    Checksum:iF5DA22C978E2C39D
    GO

    Sequence cautioni

    The sequence AAD36708.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 8114PKTSN…VFFNG → TQNFQQSFTRFFQR(PubMed:7789791)CuratedAdd
    BLAST
    Sequence conflicti108 – 1081T → S(PubMed:7789791)Curated
    Sequence conflicti138 – 1392FE → LQ(PubMed:7789791)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81845 Genomic DNA. Translation: CAA57437.1.
    Y11021 Genomic DNA. Translation: CAA71903.1.
    AE000512 Genomic DNA. Translation: AAD36708.1. Different initiation.
    PIRiA72231.
    RefSeqiNP_229441.1. NC_000853.1.
    YP_007977999.1. NC_021214.1.

    Genome annotation databases

    EnsemblBacteriaiAAD36708; AAD36708; TM_1641.
    GeneIDi896828.
    KEGGitma:TM1641.
    tmm:Tmari_1650.
    PATRICi23938256. VBITheMar51294_1660.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X81845 Genomic DNA. Translation: CAA57437.1 .
    Y11021 Genomic DNA. Translation: CAA71903.1 .
    AE000512 Genomic DNA. Translation: AAD36708.1 . Different initiation.
    PIRi A72231.
    RefSeqi NP_229441.1. NC_000853.1.
    YP_007977999.1. NC_021214.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CZ3 X-ray 2.10 A/B 2-169 [» ]
    1D1G X-ray 2.10 A/B 2-169 [» ]
    ProteinModelPortali Q60034.
    SMRi Q60034. Positions 2-169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM1641.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD36708 ; AAD36708 ; TM_1641 .
    GeneIDi 896828.
    KEGGi tma:TM1641.
    tmm:Tmari_1650.
    PATRICi 23938256. VBITheMar51294_1660.

    Phylogenomic databases

    eggNOGi COG0262.
    KOi K00287.
    OMAi KGYERVA.
    OrthoDBi EOG6KT2V2.

    Enzyme and pathway databases

    UniPathwayi UPA00077 ; UER00158 .
    BioCyci MetaCyc:MONOMER-461.
    TMAR243274:GC6P-1687-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q60034.

    Family and domain databases

    Gene3Di 3.40.430.10. 1 hit.
    InterProi IPR012259. DHFR.
    IPR024072. DHFR-like_dom.
    IPR001796. DHFR_dom.
    [Graphical view ]
    Pfami PF00186. DHFR_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00070. DHFR.
    SUPFAMi SSF53597. SSF53597. 1 hit.
    PROSITEi PS51330. DHFR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The dihydrofolate reductase-encoding gene dyrA of the hyperthermophilic bacterium Thermotoga maritima."
      van de Casteele M., Legrain C., Wilquet V., Glansdorff N.
      Gene 158:101-105(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Homo-dimeric recombinant dihydrofolate reductase from Thermotoga maritima shows extreme intrinsic stability."
      Dams T., Schurig H., Jaenicke R.
      Biol. Chem. 379:367-371(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    4. "The crystal structure of dihydrofolate reductase from Thermotoga maritima: molecular features of thermostability."
      Dams T., Auerbach G., Bader G., Jacob U., Ploom T., Huber R., Jaenicke R.
      J. Mol. Biol. 297:659-672(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH METHOTREXATE AND NADPH.

    Entry informationi

    Entry nameiDYR_THEMA
    AccessioniPrimary (citable) accession number: Q60034
    Secondary accession number(s): P96109
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3