Reviewed, UniProtKB/Swiss-Prot Q60030 (DHLE_THEIN)

Names and origin

Protein names

Recommended name:
    Leucine dehydrogenase
      Short name=LeuDH
    EC=1.4.1.9

Gene names

Name:

ldh

Organism

Thermoactinomyces intermedius

Taxonomic identifier

2024 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Thermoactinomycetaceae › Thermoactinomyces

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Functions catabolically in the bacterial metabolism of branched-chain L-amino acids. Catalyzes the reversible deamination of these branched-chain L-amino acids to their oxo analogs.
Catalytic activity	L-leucine + H(2)O + NAD(+) = 4-methyl-2-oxopentanoate + NH(3) + NADH.
Pathway	Amino-acid degradation; L-leucine degradation; 4-methyl-2-oxopentanoic acid from L-leucine (dehydrogenase route): step 1/1.
Subunit structure	Homooctamer.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
Biophysicochemical properties	Temperature dependence: Thermostable.

Ontologies

Keywords
Biological process	Branched-chain amino acid catabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	branched chain family amino acid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro leucine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Leucine dehydrogenase		PRO_0000182805
Regions
	Nucleotide binding	180 – 186	7	NAD Potential
Sites
	Active site	80	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q60030-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4830BF118AD656AE	FASTA	366	40,586
	10 20 30 40 50 60 MKIFDYMEKY DYEQLVMCQD KESGLKAIIC IHVTTLGPAL GGMRMWTYAS EEEAIEDALR 70 80 90 100 110 120 LGRGMTYKNA AAGLNLGGGK TVIIGDPRKD KNEAMFRALG RFIQGLNGRY ITAEDVGTTV 130 140 150 160 170 180 EDMDIIHEET RYVTGVSPAF GSSGNPSPVT AYGVYRGMKA AAKEAFGDDS LEGKVVAVQG 190 200 210 220 230 240 VGHVAYELCK HLHNEGAKLI VTDINKENAD RAVQEFGAEF VHPDKIYDVE CDIFAPCALG 250 260 270 280 290 300 AIINDETIER LKCKVVAGSA NNQLKEERHG KMLEEKGIVY APDYVINAGG VINVADELLG 310 320 330 340 350 360 YNRERAMKKV EGIYDKILKV FEIAKRDGIP SYLAADRMAE ERIEMMRKTR STFLQDQRNL INFNNK

References

[1]

"The purification, characterization, cloning and sequencing of the gene for a halostable and thermostable leucine dehydrogenase from Thermoactinomyces intermedius." Ohshima T., Nishida N., Bakthavatsalam S., Kataoka K., Takada H., Yoshimura T., Esaki N., Soda K. Eur. J. Biochem. 222:305-312(1994) [PubMed: 8020469] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-42; 69-89; 266-272 AND 309-317. Strain: ATCC 33205 / DSM 43846 / IFO 14230 / JCM 3312 / T-323.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79068 Genomic DNA. Translation: CAA55671.1.
PIR	S45607.
3D structure databases
HSSP	HSSP built from PDB template 1C1D based on UniProtKB Q59771.
SMR	Q60030. Positions 1-362.
ModBase	Search...
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR016211. Glu/Phe/Leu/Val_DHase_bac/arc. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000188. Phe_leu_dh. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHLE_THEIN

Accession

Primary (citable) accession number: Q60030

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)