Q60019 (NQO8_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit 8
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit 8
NDH-1 subunit 8

Gene names

Name:	nqo8
Ordered Locus Names:	TTHA0091

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. HAMAP-Rule MF_01350
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01350
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.
Subcellular location	Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_01350.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 365

365

NADH-quinone oxidoreductase subunit 8 HAMAP-Rule MF_01350

PRO_0000117522

Regions

Transmembrane

11 – 31

21

Helical; Potential

Transmembrane

80 – 100

21

Helical; Potential

Transmembrane

120 – 140

21

Helical; Potential

Transmembrane

157 – 177

21

Helical; Potential

Transmembrane

192 – 212

21

Helical; Potential

Transmembrane

252 – 272

21

Helical; Potential

Transmembrane

273 – 293

21

Helical; Potential

Transmembrane

310 – 330

21

Helical; Potential

Transmembrane

336 – 356

21

Helical; Potential

Secondary structure

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365

Helix Strand Turn

Details...

Helix

10 – 38

29

Beta strand

53 – 55

3

Helix

58 – 66

9

Helix

80 – 94

15

Beta strand

97 – 100

4

Turn

105 – 109

5

Beta strand

113 – 115

3

Helix

121 – 143

23

Helix

148 – 177

30

Helix

182 – 191

10

Helix

195 – 197

3

Helix

200 – 212

13

Turn

213 – 215

3

Beta strand

216 – 218

3

Turn

220 – 222

3

Beta strand

231 – 235

5

Helix

237 – 262

26

Beta strand

270 – 272

3

Helix

275 – 295

21

Helix

303 – 309

7

Turn

310 – 312

3

Helix

313 – 329

17

Helix

335 – 350

16

Sequences

	Sequence		Length	Mass (Da)
	Q60019 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: AE920CC029333C09	FASTA	365	41,009
	10 20 30 40 50 60 MTWSYPVDPY WMVALKALLV VVGLLTAFAF MTLIERRLLA RFQVRMGPNR VGPFGLLQPL 70 80 90 100 110 120 ADAIKSIFKE DIVVAQADRF LFVLAPLISV VFALLAFGLI PFGPPGSFFG YQPWVINLDL 130 140 150 160 170 180 GILYLFAVSE LAVYGIFLSG WASGSKYSLL GSLRSSASLI SYELGLGLAL LAPVLLVGSL 190 200 210 220 230 240 NLNDIVNWQK EHGWLFLYAF PAFLVYLIAS MAEAARTPFD LPEAEQELVG GYHTEYSSIK 250 260 270 280 290 300 WALFQMAEYI HFITASALIP TLFLGGWTMP VLEVPYLWMF LKIAFFLFFF IWIRATWFRL 310 320 330 340 350 360 RYDQLLRFGW GFLFPLALLW FLVTALVVAL DLPRTYLLYL SALSFLVLLG AVLYTPKPAR KGGGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit." Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T. J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U52917 Genomic DNA. Translation: AAA97945.1.
AP008226 Genomic DNA. Translation: BAD69914.1.

PIR

T11905.

RefSeq

YP_143357.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4HE8	X-ray	3.30	C/H	1-365	[»]
4HEA	X-ray	3.30	H/Q	1-365	[»]

ProteinModelPortal

Q60019.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59266N.

STRING

300852.TTHA0091.

Protein family/group databases

TCDB

3.D.1.3.1. H+ or Na+-translocating NADH dehydrogenase (NDH) family.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD69914; BAD69914; BAD69914.

GeneID

3169634.

KEGG

ttj:TTHA0091.

PATRIC

23955113. VBITheThe93045_0089.

Phylogenomic databases

eggNOG

COG1005.

HOGENOM

HOG000228276.

KO

K00337.

OMA

AYLAVAH.

ProtClustDB

CLSK445100.

Family and domain databases

HAMAP

MF_01350. NDH1_NuoH.

InterPro

IPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]

PANTHER

PTHR11432. PTHR11432. 1 hit.

Pfam

PF00146. NADHdh. 1 hit.
[Graphical view]

PROSITE

PS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NQO8_THET8

Accession

Primary (citable) accession number: Q60019
Secondary accession number(s): Q5SM52

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program