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Q60019

- NQO8_THET8

UniProt

Q60019 - NQO8_THET8

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Protein

NADH-quinone oxidoreductase subunit 8

Gene

nqo8

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + quinone = NAD+ + quinol.

GO - Molecular functioni

  1. NADH dehydrogenase (quinone) activity Source: UniProtKB-EC
  2. quinone binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-95-MONOMER.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 8 (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I subunit 8
NDH-1 subunit 8
Gene namesi
Name:nqo8
Ordered Locus Names:TTHA0091
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121HelicalSequence AnalysisAdd
BLAST
Transmembranei80 – 10021HelicalSequence AnalysisAdd
BLAST
Transmembranei120 – 14021HelicalSequence AnalysisAdd
BLAST
Transmembranei157 – 17721HelicalSequence AnalysisAdd
BLAST
Transmembranei192 – 21221HelicalSequence AnalysisAdd
BLAST
Transmembranei252 – 27221HelicalSequence AnalysisAdd
BLAST
Transmembranei273 – 29321HelicalSequence AnalysisAdd
BLAST
Transmembranei310 – 33021HelicalSequence AnalysisAdd
BLAST
Transmembranei336 – 35621HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365NADH-quinone oxidoreductase subunit 8PRO_0000117522Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Protein-protein interaction databases

DIPiDIP-59266N.
STRINGi300852.TTHA0091.

Structurei

Secondary structure

1
365
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 3829Combined sources
Beta strandi53 – 553Combined sources
Helixi58 – 669Combined sources
Helixi80 – 9415Combined sources
Beta strandi97 – 1004Combined sources
Turni105 – 1095Combined sources
Beta strandi113 – 1153Combined sources
Helixi121 – 14323Combined sources
Helixi148 – 17730Combined sources
Helixi182 – 19110Combined sources
Helixi195 – 1973Combined sources
Helixi200 – 21213Combined sources
Turni213 – 2153Combined sources
Beta strandi216 – 2183Combined sources
Turni220 – 2223Combined sources
Beta strandi231 – 2355Combined sources
Helixi237 – 26226Combined sources
Beta strandi270 – 2723Combined sources
Helixi275 – 29521Combined sources
Helixi303 – 3097Combined sources
Turni310 – 3123Combined sources
Helixi313 – 32917Combined sources
Helixi335 – 35016Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30C/H1-365[»]
4HEAX-ray3.30H/Q1-365[»]
ProteinModelPortaliQ60019.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1005.
HOGENOMiHOG000228276.
KOiK00337.
OMAiGAFRNFA.
OrthoDBiEOG6B62B4.
PhylomeDBiQ60019.

Family and domain databases

HAMAPiMF_01350. NDH1_NuoH.
InterProiIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERiPTHR11432. PTHR11432. 1 hit.
PfamiPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEiPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60019-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTWSYPVDPY WMVALKALLV VVGLLTAFAF MTLIERRLLA RFQVRMGPNR
60 70 80 90 100
VGPFGLLQPL ADAIKSIFKE DIVVAQADRF LFVLAPLISV VFALLAFGLI
110 120 130 140 150
PFGPPGSFFG YQPWVINLDL GILYLFAVSE LAVYGIFLSG WASGSKYSLL
160 170 180 190 200
GSLRSSASLI SYELGLGLAL LAPVLLVGSL NLNDIVNWQK EHGWLFLYAF
210 220 230 240 250
PAFLVYLIAS MAEAARTPFD LPEAEQELVG GYHTEYSSIK WALFQMAEYI
260 270 280 290 300
HFITASALIP TLFLGGWTMP VLEVPYLWMF LKIAFFLFFF IWIRATWFRL
310 320 330 340 350
RYDQLLRFGW GFLFPLALLW FLVTALVVAL DLPRTYLLYL SALSFLVLLG
360
AVLYTPKPAR KGGGA
Length:365
Mass (Da):41,009
Last modified:November 1, 1997 - v1
Checksum:iAE920CC029333C09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97945.1.
AP008226 Genomic DNA. Translation: BAD69914.1.
PIRiT11905.
RefSeqiWP_011227702.1. NC_006461.1.
YP_143357.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69914; BAD69914; BAD69914.
GeneIDi3169634.
KEGGittj:TTHA0091.
PATRICi23955113. VBITheThe93045_0089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97945.1 .
AP008226 Genomic DNA. Translation: BAD69914.1 .
PIRi T11905.
RefSeqi WP_011227702.1. NC_006461.1.
YP_143357.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HE8 X-ray 3.30 C/H 1-365 [» ]
4HEA X-ray 3.30 H/Q 1-365 [» ]
ProteinModelPortali Q60019.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-59266N.
STRINGi 300852.TTHA0091.

Protein family/group databases

TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD69914 ; BAD69914 ; BAD69914 .
GeneIDi 3169634.
KEGGi ttj:TTHA0091.
PATRICi 23955113. VBITheThe93045_0089.

Phylogenomic databases

eggNOGi COG1005.
HOGENOMi HOG000228276.
KOi K00337.
OMAi GAFRNFA.
OrthoDBi EOG6B62B4.
PhylomeDBi Q60019.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-95-MONOMER.

Family and domain databases

HAMAPi MF_01350. NDH1_NuoH.
InterProi IPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view ]
PANTHERi PTHR11432. PTHR11432. 1 hit.
Pfami PF00146. NADHdh. 1 hit.
[Graphical view ]
PROSITEi PS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
    Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
    J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiNQO8_THET8
AccessioniPrimary (citable) accession number: Q60019
Secondary accession number(s): Q5SM52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3