Reviewed, UniProtKB/Swiss-Prot Q60019 (NQO8_THET8)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit 8
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit 8
    NDH-1 subunit 8

Gene names

Name:	nqo8
Ordered Locus Names:	TTHA0091

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Complete proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. HAMAP MF_01350
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01350
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. HAMAP MF_01350
Subcellular location	Cell inner membrane; Multi-pass membrane protein. HAMAP MF_01350
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	NADH-quinone oxidoreductase subunit 8 HAMAP MF_01350		PRO_0000117522
Regions
	Transmembrane	11 – 31	21	Potential
	Transmembrane	80 – 100	21	Potential
	Transmembrane	120 – 140	21	Potential
	Transmembrane	157 – 177	21	Potential
	Transmembrane	192 – 212	21	Potential
	Transmembrane	252 – 272	21	Potential
	Transmembrane	273 – 293	21	Potential
	Transmembrane	310 – 330	21	Potential
	Transmembrane	336 – 356	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q60019-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: AE920CC029333C09	FASTA	365	41,009
	10 20 30 40 50 60 MTWSYPVDPY WMVALKALLV VVGLLTAFAF MTLIERRLLA RFQVRMGPNR VGPFGLLQPL 70 80 90 100 110 120 ADAIKSIFKE DIVVAQADRF LFVLAPLISV VFALLAFGLI PFGPPGSFFG YQPWVINLDL 130 140 150 160 170 180 GILYLFAVSE LAVYGIFLSG WASGSKYSLL GSLRSSASLI SYELGLGLAL LAPVLLVGSL 190 200 210 220 230 240 NLNDIVNWQK EHGWLFLYAF PAFLVYLIAS MAEAARTPFD LPEAEQELVG GYHTEYSSIK 250 260 270 280 290 300 WALFQMAEYI HFITASALIP TLFLGGWTMP VLEVPYLWMF LKIAFFLFFF IWIRATWFRL 310 320 330 340 350 360 RYDQLLRFGW GFLFPLALLW FLVTALVVAL DLPRTYLLYL SALSFLVLLG AVLYTPKPAR KGGGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit." Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T. J. Biol. Chem. 272:4201-4211(1997) [PubMed: 9020134] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U52917 Genomic DNA. Translation: AAA97945.1. AP008226 Genomic DNA. Translation: BAD69914.1.
PIR	T11905.
RefSeq	YP_143357.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.3.1. proton-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
GeneID	3169634.
GenomeReviews	Gene locus TTHA0091 in contig AP008226_GR.
KEGG	ttj:TTHA0091.
NMPDR	fig|300852.3.peg.122.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q60019.
OMA	Q60019. KEDIIPQ.
Enzyme and pathway databases
BioCyc	TTHE300852:TTHA0091-MON.
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NQO8_THET8

Accession

Primary (citable) accession number: Q60019
Secondary accession number(s): Q5SM52

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)