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Protein

NADH-quinone oxidoreductase subunit 8

Gene

nqo8

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 8 (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I subunit 8
NDH-1 subunit 8
Gene namesi
Name:nqo8
Ordered Locus Names:TTHA0091
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31HelicalSequence analysisAdd BLAST21
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Transmembranei157 – 177HelicalSequence analysisAdd BLAST21
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Transmembranei252 – 272HelicalSequence analysisAdd BLAST21
Transmembranei273 – 293HelicalSequence analysisAdd BLAST21
Transmembranei310 – 330HelicalSequence analysisAdd BLAST21
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001175221 – 365NADH-quinone oxidoreductase subunit 8Add BLAST365

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Protein-protein interaction databases

DIPiDIP-59266N.
STRINGi300852.TTHA0091.

Structurei

Secondary structure

1365
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 38Combined sources29
Beta strandi53 – 55Combined sources3
Helixi58 – 66Combined sources9
Helixi80 – 94Combined sources15
Beta strandi97 – 100Combined sources4
Turni105 – 109Combined sources5
Beta strandi113 – 115Combined sources3
Helixi121 – 143Combined sources23
Helixi148 – 177Combined sources30
Helixi182 – 191Combined sources10
Helixi195 – 197Combined sources3
Helixi200 – 212Combined sources13
Turni213 – 215Combined sources3
Beta strandi216 – 218Combined sources3
Turni220 – 222Combined sources3
Beta strandi231 – 235Combined sources5
Helixi237 – 262Combined sources26
Beta strandi270 – 272Combined sources3
Helixi275 – 295Combined sources21
Helixi303 – 309Combined sources7
Turni310 – 312Combined sources3
Helixi313 – 329Combined sources17
Helixi335 – 350Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30C/H1-365[»]
4HEAX-ray3.30H/Q1-365[»]
ProteinModelPortaliQ60019.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 1 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CMZ. Bacteria.
COG1005. LUCA.
HOGENOMiHOG000228276.
KOiK00337.
OMAiLDLGWKF.
PhylomeDBiQ60019.

Family and domain databases

HAMAPiMF_01350. NDH1_NuoH. 1 hit.
InterProiIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERiPTHR11432. PTHR11432. 1 hit.
PfamiPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEiPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q60019-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTWSYPVDPY WMVALKALLV VVGLLTAFAF MTLIERRLLA RFQVRMGPNR
60 70 80 90 100
VGPFGLLQPL ADAIKSIFKE DIVVAQADRF LFVLAPLISV VFALLAFGLI
110 120 130 140 150
PFGPPGSFFG YQPWVINLDL GILYLFAVSE LAVYGIFLSG WASGSKYSLL
160 170 180 190 200
GSLRSSASLI SYELGLGLAL LAPVLLVGSL NLNDIVNWQK EHGWLFLYAF
210 220 230 240 250
PAFLVYLIAS MAEAARTPFD LPEAEQELVG GYHTEYSSIK WALFQMAEYI
260 270 280 290 300
HFITASALIP TLFLGGWTMP VLEVPYLWMF LKIAFFLFFF IWIRATWFRL
310 320 330 340 350
RYDQLLRFGW GFLFPLALLW FLVTALVVAL DLPRTYLLYL SALSFLVLLG
360
AVLYTPKPAR KGGGA
Length:365
Mass (Da):41,009
Last modified:November 1, 1997 - v1
Checksum:iAE920CC029333C09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97945.1.
AP008226 Genomic DNA. Translation: BAD69914.1.
PIRiT11905.
RefSeqiWP_011227702.1. NC_006461.1.
YP_143357.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69914; BAD69914; BAD69914.
GeneIDi3169634.
KEGGittj:TTHA0091.
PATRICi23955113. VBITheThe93045_0089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52917 Genomic DNA. Translation: AAA97945.1.
AP008226 Genomic DNA. Translation: BAD69914.1.
PIRiT11905.
RefSeqiWP_011227702.1. NC_006461.1.
YP_143357.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30C/H1-365[»]
4HEAX-ray3.30H/Q1-365[»]
ProteinModelPortaliQ60019.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59266N.
STRINGi300852.TTHA0091.

Protein family/group databases

TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69914; BAD69914; BAD69914.
GeneIDi3169634.
KEGGittj:TTHA0091.
PATRICi23955113. VBITheThe93045_0089.

Phylogenomic databases

eggNOGiENOG4105CMZ. Bacteria.
COG1005. LUCA.
HOGENOMiHOG000228276.
KOiK00337.
OMAiLDLGWKF.
PhylomeDBiQ60019.

Family and domain databases

HAMAPiMF_01350. NDH1_NuoH. 1 hit.
InterProiIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERiPTHR11432. PTHR11432. 1 hit.
PfamiPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEiPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQO8_THET8
AccessioniPrimary (citable) accession number: Q60019
Secondary accession number(s): Q5SM52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.