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Q60019

- NQO8_THET8

UniProt

Q60019 - NQO8_THET8

Protein

NADH-quinone oxidoreductase subunit 8

Gene

nqo8

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP.

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.

    GO - Molecular functioni

    1. NADH dehydrogenase (quinone) activity Source: UniProtKB-EC
    2. quinone binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-95-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 8 (EC:1.6.99.5)
    Alternative name(s):
    NADH dehydrogenase I subunit 8
    NDH-1 subunit 8
    Gene namesi
    Name:nqo8
    Ordered Locus Names:TTHA0091
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 365365NADH-quinone oxidoreductase subunit 8PRO_0000117522Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

    Protein-protein interaction databases

    DIPiDIP-59266N.
    STRINGi300852.TTHA0091.

    Structurei

    Secondary structure

    1
    365
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi10 – 3829
    Beta strandi53 – 553
    Helixi58 – 669
    Helixi80 – 9415
    Beta strandi97 – 1004
    Turni105 – 1095
    Beta strandi113 – 1153
    Helixi121 – 14323
    Helixi148 – 17730
    Helixi182 – 19110
    Helixi195 – 1973
    Helixi200 – 21213
    Turni213 – 2153
    Beta strandi216 – 2183
    Turni220 – 2223
    Beta strandi231 – 2355
    Helixi237 – 26226
    Beta strandi270 – 2723
    Helixi275 – 29521
    Helixi303 – 3097
    Turni310 – 3123
    Helixi313 – 32917
    Helixi335 – 35016

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HE8X-ray3.30C/H1-365[»]
    4HEAX-ray3.30H/Q1-365[»]
    ProteinModelPortaliQ60019.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei11 – 3121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei80 – 10021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei120 – 14021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei157 – 17721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei192 – 21221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei252 – 27221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei273 – 29321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei310 – 33021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei336 – 35621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I subunit 1 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1005.
    HOGENOMiHOG000228276.
    KOiK00337.
    OMAiGAFRNFA.
    OrthoDBiEOG6B62B4.
    PhylomeDBiQ60019.

    Family and domain databases

    HAMAPiMF_01350. NDH1_NuoH.
    InterProiIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
    IPR018086. NADH_UbQ_OxRdtase_su1_CS.
    [Graphical view]
    PANTHERiPTHR11432. PTHR11432. 1 hit.
    PfamiPF00146. NADHdh. 1 hit.
    [Graphical view]
    PROSITEiPS00667. COMPLEX1_ND1_1. 1 hit.
    PS00668. COMPLEX1_ND1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q60019-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTWSYPVDPY WMVALKALLV VVGLLTAFAF MTLIERRLLA RFQVRMGPNR    50
    VGPFGLLQPL ADAIKSIFKE DIVVAQADRF LFVLAPLISV VFALLAFGLI 100
    PFGPPGSFFG YQPWVINLDL GILYLFAVSE LAVYGIFLSG WASGSKYSLL 150
    GSLRSSASLI SYELGLGLAL LAPVLLVGSL NLNDIVNWQK EHGWLFLYAF 200
    PAFLVYLIAS MAEAARTPFD LPEAEQELVG GYHTEYSSIK WALFQMAEYI 250
    HFITASALIP TLFLGGWTMP VLEVPYLWMF LKIAFFLFFF IWIRATWFRL 300
    RYDQLLRFGW GFLFPLALLW FLVTALVVAL DLPRTYLLYL SALSFLVLLG 350
    AVLYTPKPAR KGGGA 365
    Length:365
    Mass (Da):41,009
    Last modified:November 1, 1997 - v1
    Checksum:iAE920CC029333C09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97945.1.
    AP008226 Genomic DNA. Translation: BAD69914.1.
    PIRiT11905.
    RefSeqiWP_011227702.1. NC_006461.1.
    YP_143357.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD69914; BAD69914; BAD69914.
    GeneIDi3169634.
    KEGGittj:TTHA0091.
    PATRICi23955113. VBITheThe93045_0089.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52917 Genomic DNA. Translation: AAA97945.1 .
    AP008226 Genomic DNA. Translation: BAD69914.1 .
    PIRi T11905.
    RefSeqi WP_011227702.1. NC_006461.1.
    YP_143357.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HE8 X-ray 3.30 C/H 1-365 [» ]
    4HEA X-ray 3.30 H/Q 1-365 [» ]
    ProteinModelPortali Q60019.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59266N.
    STRINGi 300852.TTHA0091.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD69914 ; BAD69914 ; BAD69914 .
    GeneIDi 3169634.
    KEGGi ttj:TTHA0091.
    PATRICi 23955113. VBITheThe93045_0089.

    Phylogenomic databases

    eggNOGi COG1005.
    HOGENOMi HOG000228276.
    KOi K00337.
    OMAi GAFRNFA.
    OrthoDBi EOG6B62B4.
    PhylomeDBi Q60019.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-95-MONOMER.

    Family and domain databases

    HAMAPi MF_01350. NDH1_NuoH.
    InterProi IPR001694. NADH_UbQ_OxRdtase_su1/FPO.
    IPR018086. NADH_UbQ_OxRdtase_su1_CS.
    [Graphical view ]
    PANTHERi PTHR11432. PTHR11432. 1 hit.
    Pfami PF00146. NADHdh. 1 hit.
    [Graphical view ]
    PROSITEi PS00667. COMPLEX1_ND1_1. 1 hit.
    PS00668. COMPLEX1_ND1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
      Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
      J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.

    Entry informationi

    Entry nameiNQO8_THET8
    AccessioniPrimary (citable) accession number: Q60019
    Secondary accession number(s): Q5SM52
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3