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Q60019 (NQO8_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 8

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit 8
NDH-1 subunit 8
Gene names
Name:nqo8
Ordered Locus Names:TTHA0091
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. HAMAP-Rule MF_01350

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01350

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers.

Subcellular location

Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_01350.

Sequence similarities

Belongs to the complex I subunit 1 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365NADH-quinone oxidoreductase subunit 8 HAMAP-Rule MF_01350
PRO_0000117522

Regions

Transmembrane11 – 3121Helical; Potential
Transmembrane80 – 10021Helical; Potential
Transmembrane120 – 14021Helical; Potential
Transmembrane157 – 17721Helical; Potential
Transmembrane192 – 21221Helical; Potential
Transmembrane252 – 27221Helical; Potential
Transmembrane273 – 29321Helical; Potential
Transmembrane310 – 33021Helical; Potential
Transmembrane336 – 35621Helical; Potential

Secondary structure

........................................... 365
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q60019 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: AE920CC029333C09

FASTA36541,009
        10         20         30         40         50         60 
MTWSYPVDPY WMVALKALLV VVGLLTAFAF MTLIERRLLA RFQVRMGPNR VGPFGLLQPL 

        70         80         90        100        110        120 
ADAIKSIFKE DIVVAQADRF LFVLAPLISV VFALLAFGLI PFGPPGSFFG YQPWVINLDL 

       130        140        150        160        170        180 
GILYLFAVSE LAVYGIFLSG WASGSKYSLL GSLRSSASLI SYELGLGLAL LAPVLLVGSL 

       190        200        210        220        230        240 
NLNDIVNWQK EHGWLFLYAF PAFLVYLIAS MAEAARTPFD LPEAEQELVG GYHTEYSSIK 

       250        260        270        280        290        300 
WALFQMAEYI HFITASALIP TLFLGGWTMP VLEVPYLWMF LKIAFFLFFF IWIRATWFRL 

       310        320        330        340        350        360 
RYDQLLRFGW GFLFPLALLW FLVTALVVAL DLPRTYLLYL SALSFLVLLG AVLYTPKPAR 


KGGGA 

« Hide

References

« Hide 'large scale' references
[1]"The proton-translocating NADH-quinone oxidoreductase (NDH-1) of thermophilic bacterium Thermus thermophilus HB-8. Complete DNA sequence of the gene cluster and thermostable properties of the expressed NQO2 subunit."
Yano T., Chu S.S., Sled' V.D., Ohnishi T., Yagi T.
J. Biol. Chem. 272:4201-4211(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U52917 Genomic DNA. Translation: AAA97945.1.
AP008226 Genomic DNA. Translation: BAD69914.1.
PIRT11905.
RefSeqYP_143357.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4HE8X-ray3.30C/H1-365[»]
4HEAX-ray3.30H/Q1-365[»]
ProteinModelPortalQ60019.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59266N.
STRING300852.TTHA0091.

Protein family/group databases

TCDB3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD69914; BAD69914; BAD69914.
GeneID3169634.
KEGGttj:TTHA0091.
PATRIC23955113. VBITheThe93045_0089.

Phylogenomic databases

eggNOGCOG1005.
HOGENOMHOG000228276.
KOK00337.
OMAGAFRNFA.
OrthoDBEOG6B62B4.
PhylomeDBQ60019.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-95-MONOMER.

Family and domain databases

HAMAPMF_01350. NDH1_NuoH.
InterProIPR001694. NADH_UbQ_OxRdtase_su1/FPO.
IPR018086. NADH_UbQ_OxRdtase_su1_CS.
[Graphical view]
PANTHERPTHR11432. PTHR11432. 1 hit.
PfamPF00146. NADHdh. 1 hit.
[Graphical view]
PROSITEPS00667. COMPLEX1_ND1_1. 1 hit.
PS00668. COMPLEX1_ND1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNQO8_THET8
AccessionPrimary (citable) accession number: Q60019
Secondary accession number(s): Q5SM52
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references