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Protein

Phosphocholine transferase AnkX

Gene

ankX

Organism
Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Virulence effector that plays a role in hijacking the host vesicular trafficking by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). Acts as a phosphocholine transferase by mediating the addition of phosphocholine to Ser residues of host RAB1 (RAB1A, RAB1B or RAB1C) and RAB35, leading to displacement of GDP dissociation inhibitors (GDI). Phosphocholination of target proteins also impairs accessibility to GTPase effector LepB. Can act on both GDP-bound and GTP-bound Rab proteins.5 Publications

Catalytic activityi

CDP-choline + protein-serine = CMP + protein-serine-choline phosphate.1 Publication

Kineticsi

  1. KM=122 µM for RAB1B1 Publication

    GO - Molecular functioni

    • phosphocholine transferase activity Source: UniProtKB

    GO - Biological processi

    • metabolic process Source: UniProtKB
    • pathogenesis Source: UniProtKB
    • regulation of GTPase activity Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Virulence

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphocholine transferase AnkX (EC:2.7.1.-)
    Short name:
    PC transferase
    Alternative name(s):
    Ankyrin repeat-containing protein X
    Gene namesi
    Name:ankX
    Synonyms:legA8
    Ordered Locus Names:lpg0695
    OrganismiLegionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
    Taxonomic identifieri272624 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella
    Proteomesi
    • UP000000609 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    • extracellular region Source: UniProtKB-SubCell
    • host cell cytoplasm Source: UniProtKB
    Complete GO annotation...

    Keywords - Cellular componenti

    Host cytoplasm, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi229H → A: Abolishes phosphocholine transferase activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004175431 – 949Phosphocholine transferase AnkXAdd BLAST949

    Proteomic databases

    PaxDbiQ5ZXN6.

    Interactioni

    Protein-protein interaction databases

    STRINGi272624.lpg0695.

    Structurei

    Secondary structure

    1949
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 4Combined sources3
    Helixi11 – 16Combined sources6
    Helixi19 – 25Combined sources7
    Turni29 – 38Combined sources10
    Helixi39 – 44Combined sources6
    Helixi48 – 64Combined sources17
    Beta strandi66 – 69Combined sources4
    Helixi74 – 84Combined sources11
    Helixi89 – 92Combined sources4
    Beta strandi106 – 109Combined sources4
    Helixi111 – 113Combined sources3
    Helixi116 – 124Combined sources9
    Helixi126 – 130Combined sources5
    Beta strandi134 – 141Combined sources8
    Beta strandi144 – 148Combined sources5
    Turni151 – 154Combined sources4
    Turni157 – 159Combined sources3
    Helixi160 – 174Combined sources15
    Beta strandi181 – 184Combined sources4
    Helixi190 – 207Combined sources18
    Helixi211 – 227Combined sources17
    Beta strandi231 – 233Combined sources3
    Helixi235 – 239Combined sources5
    Turni240 – 244Combined sources5
    Helixi245 – 250Combined sources6
    Turni262 – 265Combined sources4
    Helixi269 – 291Combined sources23
    Helixi298 – 300Combined sources3
    Helixi305 – 314Combined sources10
    Helixi318 – 324Combined sources7
    Helixi333 – 344Combined sources12
    Helixi345 – 347Combined sources3
    Beta strandi348 – 351Combined sources4
    Helixi352 – 359Combined sources8
    Helixi363 – 370Combined sources8
    Helixi375 – 378Combined sources4
    Turni388 – 390Combined sources3
    Helixi395 – 402Combined sources8
    Helixi405 – 413Combined sources9
    Helixi428 – 435Combined sources8
    Helixi438 – 449Combined sources12
    Helixi454 – 458Combined sources5
    Helixi468 – 473Combined sources6
    Turni474 – 476Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4BEPX-ray3.14A/B2-484[»]
    4BERX-ray2.60A/B2-484[»]
    4BESX-ray2.54A2-484[»]
    4BETX-ray2.55A/B2-484[»]
    ProteinModelPortaliQ5ZXN6.
    SMRiQ5ZXN6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini155 – 289FidoPROSITE-ProRule annotationAdd BLAST135
    Repeati391 – 420ANK 1Add BLAST30
    Repeati424 – 453ANK 2Add BLAST30
    Repeati464 – 494ANK 3Add BLAST31
    Repeati498 – 527ANK 4Add BLAST30
    Repeati554 – 583ANK 5Add BLAST30
    Repeati588 – 617ANK 6Add BLAST30
    Repeati658 – 687ANK 7Add BLAST30
    Repeati691 – 720ANK 8Add BLAST30
    Repeati725 – 767ANK 9Add BLAST43
    Repeati771 – 800ANK 10Add BLAST30

    Domaini

    The FIDO domain mediates the phosphocholine transferase activity.1 Publication

    Sequence similaritiesi

    Contains 10 ANK repeats.PROSITE-ProRule annotation
    Contains 1 fido domain.PROSITE-ProRule annotation

    Keywords - Domaini

    ANK repeat, Repeat

    Phylogenomic databases

    eggNOGiENOG4107PTR. Bacteria.
    COG0666. LUCA.
    HOGENOMiHOG000278898.

    Family and domain databases

    Gene3Di1.10.3290.10. 2 hits.
    1.25.40.20. 2 hits.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR003812. Fido.
    [Graphical view]
    PfamiPF12796. Ank_2. 2 hits.
    PF02661. Fic. 1 hit.
    [Graphical view]
    PRINTSiPR01415. ANKYRIN.
    SMARTiSM00248. ANK. 11 hits.
    [Graphical view]
    SUPFAMiSSF140931. SSF140931. 1 hit.
    SSF48403. SSF48403. 2 hits.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 4 hits.
    PS51459. FIDO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5ZXN6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVKIMPNLPG LYFLQAYPSE EIWRLFVDGR FWSKENGWRG YESREPGCLN
    60 70 80 90 100
    AALESLCSIA LQVEKSGEEF ELSVDLIKRI HKKCGKKVEE LQEKNPGELR
    110 120 130 140 150
    TDEPVSFGIP AGRASIKGIE EFLSLVFLTE GGAEFGPGKA GPFGPRFDKN
    160 170 180 190 200
    YFKNLNPEQI PDLAKQIYFD MCKYGHSNTN HFYLAVMKNV DVYLEKITQS
    210 220 230 240 250
    YNKEIKTAET LDEKLKIIVK HIRMYEVLHP FRDANGRTFV NNLLNILLMQ
    260 270 280 290 300
    QGLPPATFYE PNVFDLYSAE ELVVVVKEAI FNTVEIIEQS KRKTPITLYG
    310 320 330 340 350
    YHSSLEEQTK FRDMLDSPSY EKIKHMDFSD LNPEKLHLKT QKCLSSLNEQ
    360 370 380 390 400
    YPLHRGAIYL SDPGEIKLLL SNRNESQINQ QIEQGAPPIY VGKTPAHLAV
    410 420 430 440 450
    ISGNMAMLDE LIAKKADLSL QDYDGKTALH YAAECGNMQI MGKILKVVLS
    460 470 480 490 500
    QEDAIKVLNI KDNHGKTAFH YAAEFGTPEL ISALTTTEVI QINEPDNSGS
    510 520 530 540 550
    SAITLAYKNH KLKIFDELLN SGADISDELL DAIWARKDKE TLGKIIAKNE
    560 570 580 590 600
    KILLNKEAFR IAISLGSVSL VKKFLRAGVD IDIPLTKDKA TPLMLSINSG
    610 620 630 640 650
    NPKLVSYLLK KGANTRLTDT SGNSVLHYVF YSKAENREAL ANIITEKDKK
    660 670 680 690 700
    LINQPNANGN PPLYNAVVVN DLKMATILLE MGARVDFEDR LGNNILHSAM
    710 720 730 740 750
    RRCDLPIILD IVKKDSTLLH KRNSERRNPF HQALHEMHTF PSSKETEEIH
    760 770 780 790 800
    FMNLSDLLLK EGVDLNKKDI KGKTILDIAL SKQYFHLCVK LMKAGAHTNI
    810 820 830 840 850
    SSPSKFLKNS DANSILERPF KFKNDLKKEL DNNPLIAMAQ INDLYVQIKN
    860 870 880 890 900
    NRIRTPTGYA PKEGVSFFKG KSNDAKAHDE VLSVLKELYD SKLTEMLGNL
    910 920 930 940
    PGEGLEEIKR SQKFFDGELK LLIKNQDISR KVDKKSIQEA VGTSLKLKW
    Length:949
    Mass (Da):107,151
    Last modified:November 23, 2004 - v1
    Checksum:iF467E4D5631800EE
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE017354 Genomic DNA. Translation: AAU26784.1.
    RefSeqiWP_010946432.1. NC_002942.5.
    YP_094731.1. NC_002942.5.

    Genome annotation databases

    EnsemblBacteriaiAAU26784; AAU26784; lpg0695.
    GeneIDi19832260.
    KEGGilpn:lpg0695.
    PATRICi22328374. VBILegPne29832_0717.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE017354 Genomic DNA. Translation: AAU26784.1.
    RefSeqiWP_010946432.1. NC_002942.5.
    YP_094731.1. NC_002942.5.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4BEPX-ray3.14A/B2-484[»]
    4BERX-ray2.60A/B2-484[»]
    4BESX-ray2.54A2-484[»]
    4BETX-ray2.55A/B2-484[»]
    ProteinModelPortaliQ5ZXN6.
    SMRiQ5ZXN6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi272624.lpg0695.

    Proteomic databases

    PaxDbiQ5ZXN6.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAU26784; AAU26784; lpg0695.
    GeneIDi19832260.
    KEGGilpn:lpg0695.
    PATRICi22328374. VBILegPne29832_0717.

    Phylogenomic databases

    eggNOGiENOG4107PTR. Bacteria.
    COG0666. LUCA.
    HOGENOMiHOG000278898.

    Family and domain databases

    Gene3Di1.10.3290.10. 2 hits.
    1.25.40.20. 2 hits.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR003812. Fido.
    [Graphical view]
    PfamiPF12796. Ank_2. 2 hits.
    PF02661. Fic. 1 hit.
    [Graphical view]
    PRINTSiPR01415. ANKYRIN.
    SMARTiSM00248. ANK. 11 hits.
    [Graphical view]
    SUPFAMiSSF140931. SSF140931. 1 hit.
    SSF48403. SSF48403. 2 hits.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 4 hits.
    PS51459. FIDO. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiANKX_LEGPH
    AccessioniPrimary (citable) accession number: Q5ZXN6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2012
    Last sequence update: November 23, 2004
    Last modified: November 2, 2016
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.