Q5ZXI6LIPA_LEGPHLipoyl synthase2.8.1.8Lip-synLSLipoate synthaseLipoic acid synthaseSulfur insertion protein LipAlipAlpg0745Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)BacteriaPseudomonadotaGammaproteobacteriaLegionellalesLegionellaceaeLegionellaThe genomic sequence of the accidental pathogen Legionella pneumophila.NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + 4 H(+) + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [[Fe-S] cluster scaffold protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 L-methionine + N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 2 reduced [2Fe-2S]-[ferredoxin][4Fe-4S] clusterBinds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.CytoplasmBelongs to the radical SAM superfamily. Lipoyl synthase family.4Fe-4SCytoplasmIronIron-sulfurMetal-bindingReference proteomeS-adenosyl-L-methionineTransferase[4Fe-4S] cluster[4Fe-4S] cluster[4Fe-4S] cluster[4Fe-4S] cluster4Fe-4S-S-AdoMet[4Fe-4S] cluster4Fe-4S-S-AdoMet[4Fe-4S] cluster4Fe-4S-S-AdoMet[4Fe-4S] clusterMGKLIDIPIVVESGQKYKTSQGVTAIKDGIKSSGQDHERLPKPKWLRIVNHTTPAYSQVKEQVQKHRLATVCEEAKCPNISECWSHGTATIMLMGAVCTRACRFCSVDTGNPHGWLDAEEPENTAETVALMNLDYVVLTSVNRDDLPDGGANHYAKTIRAIKKRSPRTKVEALTPDFQGSERDVAVLLDSGVDVFAQNVETVERLTHPVRDNRAGYQQTLNVLAFAKKYRPDVLTKTSLMLGLGETDEEIIQTMDDLRTHHVDILTLGQYLQPTKNHLPIARYVTPETFSELRQIGLKKGFFEVASGPLVRSSYRADRVFKRDNLGLDV
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