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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei559 – 5591Aminoacyl-adenylateUniRule annotation
Binding sitei603 – 6031ATPUniRule annotation
Metal bindingi894 – 8941ZincUniRule annotation
Metal bindingi897 – 8971ZincUniRule annotation
Metal bindingi914 – 9141ZincUniRule annotation
Metal bindingi917 – 9171ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLPNE272624:GHDI-936-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:lpg0937
OrganismiLegionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Taxonomic identifieri272624 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella
ProteomesiUP000000609 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 931931Isoleucine--tRNA ligasePRO_0000098407Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272624.lpg0937.

Structurei

3D structure databases

ProteinModelPortaliQ5ZWZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi600 – 6045"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZWZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEYKDTLNL PNTSFPMKAS LSVREPEMLA DWQAKGIYQK IRKARVGSKR
60 70 80 90 100
FILHDGPPYA NGHLHCGHAL NKILKDIIIK SKTFSGFDAP FVPGWDCHGL
110 120 130 140 150
PIELNVEKKV GKAGSKISPR EFRAKCREYA ASQIDIQRDE FQRLGVLGDW
160 170 180 190 200
YNPYVTMDYH YEANIVRALG LMIKNGHLQQ GFKPVHWCID CGSALAEAEV
210 220 230 240 250
DYEDKTSPSI DVAFSAVNPS EFLNCFGTQP AVKPLILPIW TTTPWTLPAN
260 270 280 290 300
EAVCLHPEID YALIDAGNSY YIVATDLVES VMARYGISHY KTSGSAKGRV
310 320 330 340 350
FEHFKLQHPF YKRQVPVVLA EHVTTESGTG CVHTAPAHGP DDYLVGQSYQ
360 370 380 390 400
LPLINPVMAN GCFAENVELF AGISVLKANE TILTVLSERN VLLASESIRH
410 420 430 440 450
SYPHCWRHKS PMIFLATPQW FVSMDKSNLR QAIINEIDKV NWVPDWGKAR
460 470 480 490 500
ISNMVENRPD WCISRQRSWG TPMPLFVHKT TRELHPDTLE LIEKVAVMIE
510 520 530 540 550
KSGIDAWFDL DSSELLGDDA KHYDKITDTM DVWLDSGISH YSVLKHNNDL
560 570 580 590 600
DFPADVYFEG SDQHRGWFNS SLTTAVAMYG VAAYKTVLTH GYTVDAEGKK
610 620 630 640 650
LSKSKGNYVA LDKLVNQHGA DILRLWVAST DYRHEVSISE EIIKRNADAY
660 670 680 690 700
RRIRNTARFL LANLFDFNPA SDCIDAKELL ELDRWALKRC QLLQEEIITA
710 720 730 740 750
YENYHFHLIY QKIHNFCAVD MGSFYLDLIK DRQYTTAKDS IARRSCQTAM
760 770 780 790 800
YHMVKAFTIW LAPILSFTAE EIWQTIPGNN SESIFIEHWY DAWPTIDAVN
810 820 830 840 850
MEDWEQLHIV RDEVNKALEE TRQRGEIGSA LAAEVTVYAD AKALPKLTRL
860 870 880 890 900
GEELRFLFIT SEAKACPISQ SPKGLAVTDC GVSIQVTASV HEKCARCWHR
910 920 930
REDVGQNQEH PELCLRCVGN ISGYHEERLY I
Length:931
Mass (Da):105,636
Last modified:November 23, 2004 - v1
Checksum:iCF03AFB63E240166
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017354 Genomic DNA. Translation: AAU27024.1.
RefSeqiYP_094971.1. NC_002942.5.

Genome annotation databases

EnsemblBacteriaiAAU27024; AAU27024; lpg0937.
GeneIDi19832503.
KEGGilpn:lpg0937.
PATRICi22328882. VBILegPne29832_0969.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017354 Genomic DNA. Translation: AAU27024.1.
RefSeqiYP_094971.1. NC_002942.5.

3D structure databases

ProteinModelPortaliQ5ZWZ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272624.lpg0937.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU27024; AAU27024; lpg0937.
GeneIDi19832503.
KEGGilpn:lpg0937.
PATRICi22328882. VBILegPne29832_0969.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciLPNE272624:GHDI-936-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Philadelphia 1 / ATCC 33152 / DSM 7513.

Entry informationi

Entry nameiSYI_LEGPH
AccessioniPrimary (citable) accession number: Q5ZWZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 23, 2004
Last modified: February 4, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.