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Protein

Ribosomal RNA large subunit methyltransferase J

Gene

rlmJ

Organism
Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the adenine in position 2030 of 23S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + adenine(2030) in 23S rRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine(2030) in 23S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei24 – 241S-adenosyl-L-methionineUniRule annotation
Binding sitei47 – 471S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei106 – 1061S-adenosyl-L-methionineUniRule annotation
Binding sitei124 – 1241S-adenosyl-L-methionineUniRule annotation
Active sitei170 – 1701Proton acceptorUniRule annotation
Binding sitei170 – 1701S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotation, Transferase

Keywords - Biological processi

rRNA processingUniRule annotation

Keywords - Ligandi

RNA-bindingUniRule annotation, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

BioCyciLPNE272624:GHDI-1295-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA large subunit methyltransferase JUniRule annotation (EC:2.1.1.266UniRule annotation)
Alternative name(s):
23S rRNA (adenine(2030)-N6)-methyltransferaseUniRule annotation
23S rRNA m6A2030 methyltransferaseUniRule annotation
Gene namesi
Name:rlmJUniRule annotation
Ordered Locus Names:lpg1296Imported
OrganismiLegionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)Imported
Taxonomic identifieri272624 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella
Proteomesi
  • UP000000609 Componenti: Chromosome

PTM / Processingi

Proteomic databases

PaxDbiQ5ZVZ2.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei9 – 91Interaction with substrate rRNAUniRule annotation

Protein-protein interaction databases

STRINGi272624.lpg1296.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OO3X-ray2.00A7-287[»]
ProteinModelPortaliQ5ZVZ2.
SMRiQ5ZVZ2. Positions 14-284.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5ZVZ2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni149 – 1502S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the RlmJ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D6S. Bacteria.
COG2961. LUCA.
HOGENOMiHOG000262479.
KOiK07115.
OMAiTYAIWYP.
OrthoDBiEOG6Q8J0P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00934. 23SrRNA_methyltr_J.
InterProiIPR007473. RlmJ.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04378. RsmJ. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5ZVZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPSFMLSYQ HGYHAGNFAD VIKHITLTRL LAYLTHKDKP LFYLETHSGR
60 70 80 90 100
GIYDLKDKQS LKTEEYKEGI NPVWLDRENL PSLFLEYISV IKQINLNSTL
110 120 130 140 150
SYYPGSPYFA INQLRSQDRL YLCELHPTEY NFLLKLPHFN KKVYVNHTDG
160 170 180 190 200
VSKLNALLPP PEKRGLIFID PSYERKEEYK EIPYAIKNAY SKFSTGLYCV
210 220 230 240 250
WYPVVNKAWT EQFLRKMREI SSKSVRIELH LNPLINEGMT GCGLWIINPP
260 270 280
YTFPSEIKLV LETLTTYFNP GSSSYMIESG SKLCHEL
Length:287
Mass (Da):33,455
Last modified:November 23, 2004 - v1
Checksum:i8DBBE670552F0E6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017354 Genomic DNA. Translation: AAU27379.1.
RefSeqiWP_010947027.1. NC_002942.5.
YP_095326.1. NC_002942.5.

Genome annotation databases

EnsemblBacteriaiAAU27379; AAU27379; lpg1296.
GeneIDi19832861.
KEGGilpn:lpg1296.
PATRICi22329682. VBILegPne29832_1365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017354 Genomic DNA. Translation: AAU27379.1.
RefSeqiWP_010947027.1. NC_002942.5.
YP_095326.1. NC_002942.5.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OO3X-ray2.00A7-287[»]
ProteinModelPortaliQ5ZVZ2.
SMRiQ5ZVZ2. Positions 14-284.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272624.lpg1296.

Proteomic databases

PaxDbiQ5ZVZ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU27379; AAU27379; lpg1296.
GeneIDi19832861.
KEGGilpn:lpg1296.
PATRICi22329682. VBILegPne29832_1365.

Phylogenomic databases

eggNOGiENOG4105D6S. Bacteria.
COG2961. LUCA.
HOGENOMiHOG000262479.
KOiK07115.
OMAiTYAIWYP.
OrthoDBiEOG6Q8J0P.

Enzyme and pathway databases

BioCyciLPNE272624:GHDI-1295-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5ZVZ2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00934. 23SrRNA_methyltr_J.
InterProiIPR007473. RlmJ.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04378. RsmJ. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Philadelphia 1 / ATCC 33152 / DSM 7513Imported.
  2. "Crystal structure of the hypothetical lpl1258 protein from Legionella pneumophila."
    Bonanno J.B., Freeman J., Bain K.T., Slocombe A., Reyes C., Ozyurt S., Smyth L., Atwell S., Sauder J.M., Burley S.K., Almo S.C.
    Submitted (JAN-2007) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 7-287.

Entry informationi

Entry nameiQ5ZVZ2_LEGPH
AccessioniPrimary (citable) accession number: Q5ZVZ2
Entry historyi
Integrated into UniProtKB/TrEMBL: November 23, 2004
Last sequence update: November 23, 2004
Last modified: March 16, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.