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Q5ZVG8 (BIOB_LEGPH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Biotin synthase HAMAP-Rule MF_01694
PRO_0000381440

Sites

Metal binding541Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding981Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1291Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1891Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2611Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5ZVG8 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 5A48E1A8F2153AB0

FASTA31535,177
        10         20         30         40         50         60 
MSEEKKQWSI SDIEAIYQQP FNDLLYQAHT IHRTYHDPNS LQFATLLSIK TGACPEDCGY 

        70         80         90        100        110        120 
CSQSGHYKTH VEKEKLMSVE EVLQCAKEAK EGGAKRFCMG AAWRCPPDKA IPQLKEMIEG 

       130        140        150        160        170        180 
VKSLGLETCM TLGMLTKEQA SHLKEAGLDY YNHNIDTSPS YYDKVVTTRK FSDRLDTLNN 

       190        200        210        220        230        240 
VRSAGINVCC GGILGLGETR EDRIEFLLTL ANMETPPESV PINRLIPVEG TPLAQAERVE 

       250        260        270        280        290        300 
GIELVRTIAT ARILMPKSAI RLTAGRTEMS DELQALCYFA GANSVFIGDK LLTEDNPQRF 

       310 
KDKTLFNKLG LTEMV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017354 Genomic DNA. Translation: AAU27554.1.
RefSeqYP_007568272.1. NC_020521.1.
YP_095501.1. NC_002942.5.

3D structure databases

ProteinModelPortalQ5ZVG8.
SMRQ5ZVG8. Positions 6-313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272624.lpg1472.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAU27554; AAU27554; lpg1472.
GeneID14800421.
3079940.
KEGGlpn:lpg1472.
lpu:LPE509_01729.
PATRIC22330044. VBILegPne29832_1545.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycLPNE272624:GHDI-1471-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_LEGPH
AccessionPrimary (citable) accession number: Q5ZVG8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways