Reviewed, UniProtKB/Swiss-Prot Q5ZUT5 (ASTD_LEGPH)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	lpg1707

Organism

Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) [Complete proteome] [HAMAP]

Taxonomic identifier

272624 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity. HAMAP MF_01174
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD+ + H2O = N-succinyl-L-glutamate + NADH. HAMAP MF_01174
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5. HAMAP MF_01174
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	N-succinylglutamate 5-semialdehyde dehydrogenase HAMAP MF_01174		PRO_0000262407
Regions
	Nucleotide binding	229 – 234	6	NAD By similarity
Sites
	Active site	252	1	By similarity
	Active site	286	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZUT5-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: C1704C21B77D38E6	FASTA	496	54,696
	10 20 30 40 50 60 MMSKLQIIQS KGQYINGEWI KGNGLILEST NPASGTLLWQ GNNATDEEIA NACYVAHRAL 70 80 90 100 110 120 KSWANTSFEE RARYTKAFVE QVEKNREQLA RLISLETGKP LWESQTEVSS VIGKVNLSIQ 130 140 150 160 170 180 AYQERTWPKQ TETAEANACL RFKPHGIVVV LGAFNFPAHL SNGHIVPALL AGNTVLYKPS 190 200 210 220 230 240 EHTPAVAELI IQCWHDSGLP PGVINCLQGN ANCGNTLLSQ DIQGVYFTGS YATGLRIHQQ 250 260 270 280 290 300 FCNRPEVILA LEMGGNNPLV IDEVKDIDAA VYHTILSTMI TAGQRCTCAR RIIIPDSQTG 310 320 330 340 350 360 DLFLERFAKA CKLMRIGSFD SQPEPFIGPV ISHVQALKHL HAQKQLIEMG GEIILPMSLL 370 380 390 400 410 420 LEYTGLVSPG IIDMTRAKNP PDEEIFAPFA QIYRYNHFDE AIQLANQTRY GLSAGLLSDN 430 440 450 460 470 480 KDHYQQFYQN IRAGLINWNR PTTGAASSLP FGGVGCSGNH RPSAYFAADY CAYPVASMEQ 490 PLLTTPVQRL PGLVLE

References

[1]

"The genomic sequence of the accidental pathogen Legionella pneumophila." Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G., Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V., Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S. , Georghiou A., Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A., Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A., Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A., Russo J.J. Science 305:1966-1968(2004) [PubMed: 15448271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017354 Genomic DNA. Translation: AAU27787.1.
RefSeq	YP_095734.1.
3D structure databases
SMR	Q5ZUT5. Positions 13-466.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZUT5.
Genome annotation databases
GeneID	3077778.
GenomeReviews	Gene locus lpg1707 in contig AE017354_GR.
KEGG	lpn:lpg1707.
NMPDR	fig|272624.3.peg.537.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HBG752218.
OMA	KAYHART.
Enzyme and pathway databases
BioCyc	LPNE272624:LPG1707-MONOMER.
Family and domain databases
HAMAP	MF_01174. Aldedh_AstD. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR03240. arg_catab_astD. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. False negative. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_LEGPH

Accession

Primary (citable) accession number: Q5ZUT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)