Reviewed, UniProtKB/Swiss-Prot Q5ZUT4 (ASTB_LEGPH)

Names and origin

Protein names

Recommended name:
    N-succinylarginine dihydrolase
    EC=3.5.3.23

Gene names

Name:	astB
Ordered Locus Names:	lpg1708

Organism

Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) [Complete proteome] [HAMAP]

Taxonomic identifier

272624 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)-succinylornithine, ammonia and CO2 By similarity.
Catalytic activity	N(2)-succinyl-L-arginine + 2 H2O = N(2)-succinyl-L-ornithine + 2 NH3 + CO2. HAMAP MF_01172
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 2/5. HAMAP MF_01172
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the succinylarginine dihydrolase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	N-succinylarginine dihydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	N-succinylarginine dihydrolase HAMAP MF_01172		PRO_0000262357
Regions
	Region	19 – 28	10	Substrate binding By similarity
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	174	1	By similarity
	Active site	252	1	By similarity
	Active site	372	1	Nucleophile By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	216	1	Substrate By similarity
	Binding site	254	1	Substrate By similarity
	Binding site	366	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZUT4-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: C7B71B56141BE4E3	FASTA	448	50,134
	10 20 30 40 50 60 MNVYELNMDG LVGQTHHYAG LSSGNIASTN NALSISNPQA AARQGLEKMR QLYNMGLKQG 70 80 90 100 110 120 LLPPHQRPNL NLLYQLGFKG TPSEQINKAY KTAPELLSAC YSASSMWTAN AATVSASVDT 130 140 150 160 170 180 EDNKVHFTAA NLISNLHRHQ EADFSKKLLE FIFSNSDYFN HHPLLPKSMG TSDEGAANHN 190 200 210 220 230 240 RLCQSHAHSG INLFVYGKKV LGNHQFEQSP IKYPARQTKE ASEAIARNHL LNPERVIFAC 250 260 270 280 290 300 QNPLAIDQGV FHNDVISVAN EHVFLVHEEA FYNQAYVLDQ LREKADFPLV IIQISKEQIS 310 320 330 340 350 360 VSEAVDTYLF NSQLITLPDQ KNMILIAPAE CQANLKVKTC IDGLVADPQN PINSVYYLDL 370 380 390 400 410 420 KQSMRNGGGP ACLRLRVPLN DYELKAMHQG ILIDNDLLDI LDKWVLKYYR TELKIPDLAD 430 440 PQLLYECLDA LDELTQILKL GSIYPFQS

References

[1]

"The genomic sequence of the accidental pathogen Legionella pneumophila." Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G., Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V., Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S. , Georghiou A., Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A., Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A., Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A., Russo J.J. Science 305:1966-1968(2004) [PubMed: 15448271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017354 Genomic DNA. Translation: AAU27788.1.
RefSeq	YP_095735.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3078040.
GenomeReviews	Gene locus lpg1708 in contig AE017354_GR.
KEGG	lpn:lpg1708.
NMPDR	fig|272624.3.peg.538.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5ZUT4.
OMA	Q5ZUT4. HFAHHPA.
Enzyme and pathway databases
BioCyc	LPNE272624:LPG1708-MON.
Family and domain databases
HAMAP	MF_01172. [Tree]
InterPro	IPR007079. SuccinylArg_d-Hdrlase_AstB. [Graphical view]
Gene3D	G3DSA:3.75.10.20. SuccinylArg_di_hydro. 1 hit.
Pfam	PF04996. AstB. 1 hit. [Graphical view]
TIGRFAMs	TIGR03241. arg_catab_astB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTB_LEGPH

Accession

Primary (citable) accession number: Q5ZUT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 23, 2004
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)