Q5ZT84 (LLY_LEGPH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxyphenylpyruvate dioxygenase
Short name=4HPPD
Short name=HPD
Short name=HPPDase
EC=1.13.11.27

Alternative name(s):
Legiolysin

Gene names

Name:	lly
Synonyms:	hpd
Ordered Locus Names:	lpg2278

Organism

Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) [Complete proteome] [HAMAP]

Taxonomic identifier

272624 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transformation of p-hydroxyphenylpyruvate into HGA. Has hemolytic and brown pigment production activity.
Catalytic activity	4-hydroxyphenylpyruvate + O2 = homogentisate + CO2.
Cofactor	Binds 1 iron ion per subunit By similarity.
Sequence similarities	Belongs to the 4HPPD family.
Sequence caution	The sequence AAU28343.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase Toxin
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic amino acid family metabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-hydroxyphenylpyruvate dioxygenase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	4-hydroxyphenylpyruvate dioxygenase		PRO_0000088412
Sites
	Metal binding	154	1	Iron By similarity
	Metal binding	232	1	Iron By similarity
	Metal binding	312	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZT84 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: DA822AAFB019DB8F	FASTA	348	38,923
	10 20 30 40 50 60 MQNNNPCGLD GFAFLEFSGP DRNKLHQQFS EMGFQAVAHH KNQDITLFKQ GEIQFIVNAA 70 80 90 100 110 120 SHCQAEAHAS THGPGACAMG FKVKDAKAAF QHAIAHGGIA FQDAPHANHG LPAIQAIGGS 130 140 150 160 170 180 VIYFVDEEHQ PFSHEWNITS PEPVVGNGLT AIDHLTHNVY RGNMDKWASF YASIFNFQEI 190 200 210 220 230 240 RFFNIKGKMT GLVSRALGSP CGKIKIPLNE SKDDLSQIEE FLHEYHGEGI QHIALNTNDI 250 260 270 280 290 300 YKTVNGLRKQ GVKFLDVPDT YYEMINDRLP WHKEPLNQLH AEKILIDGEA DPKDGLLLQI 310 320 330 340 FTENIFGPVF FEIIQRKGNQ GFGEGNFQAL FEAIERDQVR RGTLKELS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence determination and mutational analysis of the lly locus of Legionella pneumophila." Wintermeyer E., Flugel M., Ott M., Steinert M., Rdest U., Mann K.H., Hacker J. Infect. Immun. 62:1109-1117(1994) [PubMed: 8112844] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Homogentisic acid is a product of the p-hydroxyphenylpyruvate dioxygenase activity of the Lly-protein, which mediates melanogenesis in Legionella pneumophila." Flugel M., Steinert M., Wintermeyer E., Supriyono A., Proksch P., Hacker J. Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 94, CHARACTERIZATION.
[3]	"The genomic sequence of the accidental pathogen Legionella pneumophila." Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G., Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V., Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S. , Georghiou A., Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A., Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A., Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A., Russo J.J. Science 305:1966-1968(2004) [PubMed: 15448271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Philadelphia 1 / ATCC 33152 / DSM 7513.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF075724 Genomic DNA. Translation: AAC32843.1. AE017354 Genomic DNA. Translation: AAU28343.1. Different initiation.
RefSeq	YP_096290.1. NC_002942.5.
3D structure databases
ProteinModelPortal	Q5ZT84.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZT84.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3079183.
GenomeReviews	Gene locus lpg2278 in contig AE017354_GR.
KEGG	lpn:lpg2278.
PATRIC	22331777. VBILegPne29832_2395.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3185.
HOGENOM	HBG680392.
PhylomeDB	Q5ZT84.
ProtClustDB	CLSK833301.
Enzyme and pathway databases
BioCyc	LPNE272624:LPG2278-MONOMER.
Family and domain databases
InterPro	IPR005956. 4OHPhenylPyrv_dOase. IPR004360. Glyas_Fos-R_dOase. [Graphical view]
KO	K00457.
PANTHER	PTHR11959. HPP_dOase. 1 hit.
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
PIRSF	PIRSF009283. HPP_dOase. 1 hit.
TIGRFAMs	TIGR01263. 4HPPD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LLY_LEGPH

Accession

Primary (citable) accession number: Q5ZT84
Secondary accession number(s): Q53407

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program