Reviewed, UniProtKB/Swiss-Prot Q5ZRP5 (TPMT_LEGPH)

Names and origin

Protein names

Recommended name:
    Thiopurine S-methyltransferase
    EC=2.1.1.67
Alternative name(s):
    Thiopurine methyltransferase

Gene names

Name:	tpm
Ordered Locus Names:	lpg2835

Organism

Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) [Complete proteome] [HAMAP]

Taxonomic identifier

272624 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether. HAMAP MF_00812
Subcellular location	Cytoplasm By similarity HAMAP MF_00812.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Thiopurine S-methyltransferase HAMAP MF_00812		PRO_0000220122
Sites
	Binding site	12	1	S-adenosyl-L-methionine By similarity
	Binding site	47	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	68	1	S-adenosyl-L-methionine By similarity
	Binding site	125	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZRP5-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 77E830A2C150ACBB	FASTA	221	25,383
	10 20 30 40 50 60 MNKGQYFWNE LWCEGRISFH KEEVNPDLIA YISSLNIPAK GRVLVPLCGK SVDMLWLVRQ 70 80 90 100 110 120 GYHVVGIELV EKAILQFVQE HQITVRENTT GQAKQYFTDN LNLWITDIFA LNAALIEPVD 130 140 150 160 170 180 AIYDRAALVA LPKKLRPAYV DICLKWLKPG GSILLKTLQY NQEKVQGPPY SVSPEEIALS 190 200 210 220 YQQCAKIELL KSQKRIREPN DHLFNLGISE VNDYVWRIRK G

References

[1]

"The genomic sequence of the accidental pathogen Legionella pneumophila." Chien M., Morozova I., Shi S., Sheng H., Chen J., Gomez S.M., Asamani G., Hill K., Nuara J., Feder M., Rineer J., Greenberg J.J., Steshenko V., Park S.H., Zhao B., Teplitskaya E., Edwards J.R., Pampou S. , Georghiou A., Chou I.-C., Iannuccilli W., Ulz M.E., Kim D.H., Geringer-Sameth A., Goldsberry C., Morozov P., Fischer S.G., Segal G., Qu X., Rzhetsky A., Zhang P., Cayanis E., De Jong P.J., Ju J., Kalachikov S., Shuman H.A., Russo J.J. Science 305:1966-1968(2004) [PubMed: 15448271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017354 Genomic DNA. Translation: AAU28883.1.
RefSeq	YP_096830.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZRP5.
Genome annotation databases
GeneID	3079708.
GenomeReviews	Gene locus lpg2835 in contig AE017354_GR.
KEGG	lpn:lpg2835.
NMPDR	fig|272624.3.peg.2775.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HBG444929.
OMA	GKSLDMC.
Enzyme and pathway databases
BioCyc	LPNE272624:LPG2835-MONOMER.
Family and domain databases
HAMAP	MF_00812. Thiopur_methtran. [Tree]
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_LEGPH

Accession

Primary (citable) accession number: Q5ZRP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	November 23, 2004
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)