Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CD276 antigen

Gene

CD276

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.6 Publications

GO - Molecular functioni

  • receptor binding Source: HGNC

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
CD276 antigen
Alternative name(s):
4Ig-B7-H3
B7 homolog 3
Short name:
B7-H3
Costimulatory molecule
CD_antigen: CD276
Gene namesi
Name:CD276
Synonyms:B7H3
ORF Names:PSEC0249, UNQ309/PRO352
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:19137. CD276.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 466ExtracellularSequence analysisAdd BLAST438
Transmembranei467 – 487HelicalSequence analysisAdd BLAST21
Topological domaini488 – 534CytoplasmicSequence analysisAdd BLAST47

GO - Cellular componenti

  • external side of plasma membrane Source: HGNC
  • extracellular exosome Source: UniProtKB
  • integral component of membrane Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi80381.
OpenTargetsiENSG00000103855.
PharmGKBiPA142672148.

Polymorphism and mutation databases

BioMutaiCD276.
DMDMi74757248.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 281 PublicationAdd BLAST28
ChainiPRO_000004580129 – 534CD276 antigenAdd BLAST506

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 122PROSITE-ProRule annotation
Glycosylationi104N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi165 ↔ 220PROSITE-ProRule annotation
Glycosylationi189N-linked (GlcNAc...)Sequence analysis1
Glycosylationi215N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi268 ↔ 340PROSITE-ProRule annotation
Glycosylationi322N-linked (GlcNAc...)1 Publication1
Disulfide bondi383 ↔ 438PROSITE-ProRule annotation
Glycosylationi407N-linked (GlcNAc...)1 Publication1
Glycosylationi433N-linked (GlcNAc...)Sequence analysis1
Modified residuei525PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ5ZPR3.
MaxQBiQ5ZPR3.
PaxDbiQ5ZPR3.
PeptideAtlasiQ5ZPR3.
PRIDEiQ5ZPR3.

PTM databases

iPTMnetiQ5ZPR3.
PhosphoSitePlusiQ5ZPR3.
SwissPalmiQ5ZPR3.

Expressioni

Tissue specificityi

Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.5 Publications

Inductioni

By bacterial lipopolysaccharides (LPS) in monocytes and by ionomycin in T and B-lymphocytes. Up-regulated in cells mediating rejection of human transplants.2 Publications

Gene expression databases

BgeeiENSG00000103855.
CleanExiHS_CD276.
ExpressionAtlasiQ5ZPR3. baseline and differential.
GenevisibleiQ5ZPR3. HS.

Organism-specific databases

HPAiCAB017826.
HPA009285.
HPA017139.

Interactioni

Subunit structurei

Interacts with TREML2 and this interaction enhances T-cell activation.1 Publication

GO - Molecular functioni

  • receptor binding Source: HGNC

Protein-protein interaction databases

BioGridi123260. 10 interactors.
IntActiQ5ZPR3. 1 interactor.
MINTiMINT-5000152.
STRINGi9606.ENSP00000320084.

Structurei

3D structure databases

ProteinModelPortaliQ5ZPR3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 139Ig-like V-type 1Add BLAST111
Domaini145 – 238Ig-like C2-type 1Add BLAST94
Domaini243 – 357Ig-like V-type 2Add BLAST115
Domaini363 – 456Ig-like C2-type 2Add BLAST94

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IR2H. Eukaryota.
ENOG410ZB69. LUCA.
GeneTreeiENSGT00700000104325.
HOVERGENiHBG079786.
InParanoidiQ5ZPR3.
KOiK06746.
OMAiAHSSVTI.
OrthoDBiEOG091G0IEZ.
PhylomeDBiQ5ZPR3.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003597. Ig_C1-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 2 hits.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00407. IGc1. 2 hits.
SM00408. IGc2. 4 hits.
SM00406. IGv. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 4 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5ZPR3-1) [UniParc]FASTAAdd to basket
Also known as: 4Ig-B7-H3

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRRRGSPGM GVHVGAALGA LWFCLTGALE VQVPEDPVVA LVGTDATLCC
60 70 80 90 100
SFSPEPGFSL AQLNLIWQLT DTKQLVHSFA EGQDQGSAYA NRTALFPDLL
110 120 130 140 150
AQGNASLRLQ RVRVADEGSF TCFVSIRDFG SAAVSLQVAA PYSKPSMTLE
160 170 180 190 200
PNKDLRPGDT VTITCSSYQG YPEAEVFWQD GQGVPLTGNV TTSQMANEQG
210 220 230 240 250
LFDVHSILRV VLGANGTYSC LVRNPVLQQD AHSSVTITPQ RSPTGAVEVQ
260 270 280 290 300
VPEDPVVALV GTDATLRCSF SPEPGFSLAQ LNLIWQLTDT KQLVHSFTEG
310 320 330 340 350
RDQGSAYANR TALFPDLLAQ GNASLRLQRV RVADEGSFTC FVSIRDFGSA
360 370 380 390 400
AVSLQVAAPY SKPSMTLEPN KDLRPGDTVT ITCSSYRGYP EAEVFWQDGQ
410 420 430 440 450
GVPLTGNVTT SQMANEQGLF DVHSVLRVVL GANGTYSCLV RNPVLQQDAH
460 470 480 490 500
GSVTITGQPM TFPPEALWVT VGLSVCLIAL LVALAFVCWR KIKQSCEEEN
510 520 530
AGAEDQDGEG EGSKTALQPL KHSDSKEDDG QEIA
Note: Contains tandemly repeated immunoglobulin-like V and C domains.
Length:534
Mass (Da):57,235
Last modified:November 23, 2004 - v1
Checksum:i2181A064404C7939
GO
Isoform 2 (identifier: Q5ZPR3-2) [UniParc]FASTAAdd to basket
Also known as: B7-H3

The sequence of this isoform differs from the canonical sequence as follows:
     159-376: Missing.

Note: Minor transcript. Contains one single set of immunoglobulin-like V and C domains.
Show »
Length:316
Mass (Da):33,791
Checksum:iFF97007F191CCFA1
GO
Isoform 3 (identifier: Q5ZPR3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     465-493: EALWVTVGLSVCLIALLVALAFVCWRKIK → GPASSAVPLSPAHPPHGSMCWSHWFSRGL
     494-534: Missing.

Note: Contains tandemly repeated immunoglobulin-like V and C domains.
Show »
Length:493
Mass (Da):52,761
Checksum:iCD7C5591CC4822D2
GO
Isoform 4 (identifier: Q5ZPR3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     528-534: DDGQEIA → GKDTWA

Note: Contains tandemly repeated immunoglobulin-like V and C domains.
Show »
Length:533
Mass (Da):57,165
Checksum:iBC38033295EBE978
GO

Sequence cautioni

The sequence BAC11344 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti387R → Q in AAQ88709 (PubMed:12975309).Curated1
Sequence conflicti473L → P in BAC11243 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04985797P → L.Corresponds to variant rs7173448dbSNPEnsembl.1
Natural variantiVAR_049858111R → S.Corresponds to variant rs7173476dbSNPEnsembl.1
Natural variantiVAR_049859137Q → L.Corresponds to variant rs11574477dbSNPEnsembl.1
Natural variantiVAR_049860160T → M.2 PublicationsCorresponds to variant rs11574479dbSNPEnsembl.1
Natural variantiVAR_049861267R → H.Corresponds to variant rs11574483dbSNPEnsembl.1
Natural variantiVAR_049862279A → T.Corresponds to variant rs10083681dbSNPEnsembl.1
Natural variantiVAR_049863315P → L.Corresponds to variant rs7173448dbSNPEnsembl.1
Natural variantiVAR_049864329R → S.Corresponds to variant rs7173476dbSNPEnsembl.1
Natural variantiVAR_049865378T → M.Corresponds to variant rs11574479dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017088159 – 376Missing in isoform 2. 2 PublicationsAdd BLAST218
Alternative sequenceiVSP_017089465 – 493EALWV…WRKIK → GPASSAVPLSPAHPPHGSMC WSHWFSRGL in isoform 3. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_017090494 – 534Missing in isoform 3. 1 PublicationAdd BLAST41
Alternative sequenceiVSP_017091528 – 534DDGQEIA → GKDTWA in isoform 4. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302102 mRNA. Translation: AAK15438.1.
AJ583695 mRNA. Translation: CAE47548.1.
AY358343 mRNA. Translation: AAQ88709.1.
AK074849 mRNA. Translation: BAC11243.1.
AK074997 mRNA. Translation: BAC11344.1. Different initiation.
AK075549 mRNA. Translation: BAC11692.1.
BC062581 mRNA. Translation: AAH62581.1.
CCDSiCCDS10251.1. [Q5ZPR3-2]
CCDS32288.1. [Q5ZPR3-1]
RefSeqiNP_001019907.1. NM_001024736.1. [Q5ZPR3-1]
NP_001316557.1. NM_001329628.1. [Q5ZPR3-2]
NP_001316558.1. NM_001329629.1.
NP_079516.1. NM_025240.2. [Q5ZPR3-2]
XP_005254757.1. XM_005254700.4.
XP_011520397.1. XM_011522095.2. [Q5ZPR3-1]
XP_011520398.1. XM_011522096.2. [Q5ZPR3-1]
XP_016878127.1. XM_017022638.1. [Q5ZPR3-1]
UniGeneiHs.744915.

Genome annotation databases

EnsembliENST00000318424; ENSP00000320058; ENSG00000103855. [Q5ZPR3-2]
ENST00000318443; ENSP00000320084; ENSG00000103855. [Q5ZPR3-1]
ENST00000561213; ENSP00000452736; ENSG00000103855. [Q5ZPR3-4]
ENST00000564751; ENSP00000454940; ENSG00000103855. [Q5ZPR3-2]
GeneIDi80381.
KEGGihsa:80381.
UCSCiuc002avu.2. human. [Q5ZPR3-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF302102 mRNA. Translation: AAK15438.1.
AJ583695 mRNA. Translation: CAE47548.1.
AY358343 mRNA. Translation: AAQ88709.1.
AK074849 mRNA. Translation: BAC11243.1.
AK074997 mRNA. Translation: BAC11344.1. Different initiation.
AK075549 mRNA. Translation: BAC11692.1.
BC062581 mRNA. Translation: AAH62581.1.
CCDSiCCDS10251.1. [Q5ZPR3-2]
CCDS32288.1. [Q5ZPR3-1]
RefSeqiNP_001019907.1. NM_001024736.1. [Q5ZPR3-1]
NP_001316557.1. NM_001329628.1. [Q5ZPR3-2]
NP_001316558.1. NM_001329629.1.
NP_079516.1. NM_025240.2. [Q5ZPR3-2]
XP_005254757.1. XM_005254700.4.
XP_011520397.1. XM_011522095.2. [Q5ZPR3-1]
XP_011520398.1. XM_011522096.2. [Q5ZPR3-1]
XP_016878127.1. XM_017022638.1. [Q5ZPR3-1]
UniGeneiHs.744915.

3D structure databases

ProteinModelPortaliQ5ZPR3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123260. 10 interactors.
IntActiQ5ZPR3. 1 interactor.
MINTiMINT-5000152.
STRINGi9606.ENSP00000320084.

PTM databases

iPTMnetiQ5ZPR3.
PhosphoSitePlusiQ5ZPR3.
SwissPalmiQ5ZPR3.

Polymorphism and mutation databases

BioMutaiCD276.
DMDMi74757248.

Proteomic databases

EPDiQ5ZPR3.
MaxQBiQ5ZPR3.
PaxDbiQ5ZPR3.
PeptideAtlasiQ5ZPR3.
PRIDEiQ5ZPR3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000318424; ENSP00000320058; ENSG00000103855. [Q5ZPR3-2]
ENST00000318443; ENSP00000320084; ENSG00000103855. [Q5ZPR3-1]
ENST00000561213; ENSP00000452736; ENSG00000103855. [Q5ZPR3-4]
ENST00000564751; ENSP00000454940; ENSG00000103855. [Q5ZPR3-2]
GeneIDi80381.
KEGGihsa:80381.
UCSCiuc002avu.2. human. [Q5ZPR3-1]

Organism-specific databases

CTDi80381.
DisGeNETi80381.
GeneCardsiCD276.
HGNCiHGNC:19137. CD276.
HPAiCAB017826.
HPA009285.
HPA017139.
MIMi605715. gene.
neXtProtiNX_Q5ZPR3.
OpenTargetsiENSG00000103855.
PharmGKBiPA142672148.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IR2H. Eukaryota.
ENOG410ZB69. LUCA.
GeneTreeiENSGT00700000104325.
HOVERGENiHBG079786.
InParanoidiQ5ZPR3.
KOiK06746.
OMAiAHSSVTI.
OrthoDBiEOG091G0IEZ.
PhylomeDBiQ5ZPR3.
TreeFamiTF331083.

Miscellaneous databases

ChiTaRSiCD276. human.
GeneWikiiCD276.
GenomeRNAii80381.
PROiQ5ZPR3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000103855.
CleanExiHS_CD276.
ExpressionAtlasiQ5ZPR3. baseline and differential.
GenevisibleiQ5ZPR3. HS.

Family and domain databases

Gene3Di2.60.40.10. 4 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003597. Ig_C1-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 2 hits.
[Graphical view]
SMARTiSM00409. IG. 4 hits.
SM00407. IGc1. 2 hits.
SM00408. IGc2. 4 hits.
SM00406. IGv. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 4 hits.
PROSITEiPS50835. IG_LIKE. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD276_HUMAN
AccessioniPrimary (citable) accession number: Q5ZPR3
Secondary accession number(s): Q6P5Y4
, Q6UXI2, Q8NBI8, Q8NC34, Q8NCB6, Q9BXR1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: November 23, 2004
Last modified: November 30, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.