Reviewed, UniProtKB/Swiss-Prot Q5ZMW5 (RHG26_CHICK)

Names and origin

Protein names

Recommended name:
    Rho GTPase-activating protein 26
Alternative name(s):
    Rho-type GTPase-activating protein 26
    GTPase regulator associated with focal adhesion kinase

Gene names

Name:	ARHGAP26
Synonyms:	GRAF

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	GTPase-activating protein for RHOA and CDC42. Ref.2 Ref.3
Subunit structure	Binds to the C-terminus of PTK2/FAK. Ref.2
Subcellular location	Cell junction › focal adhesion. Cytoplasm › cytoskeleton. Note: Colocalizes with actin stress fibers and cortical actin structures. Ref.2
Tissue specificity	Detected in embryonic brain and liver, and at low levels in embryonic eye, heart, lung, intestine and skeletal muscle. Ref.2
Sequence similarities	Contains 1 PH domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasm Cytoskeleton
Domain	SH3 domain
Molecular function	GTPase activation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell focal adhesion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTPase activator activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 760	760	Rho GTPase-activating protein 26		PRO_0000355552
Regions
	Domain	265 – 369	105	PH
	Domain	383 – 568	186	Rho-GAP
	Domain	702 – 760	59	SH3
Experimental info
	Sequence conflict	196	1	F → S in CAG30928. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5ZMW5-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: DE3E2F3D4ED56667	FASTA	760	86,524
	10 20 30 40 50 60 MGLPALEFSE CCLDSPQFRE RLRSHEAELE KTNKFIKELI KDGKSLIAAL KNLSSAKRKF 70 80 90 100 110 120 ADSLNEFKFR CIGDAETDDE ICIAKSLQEF ATVLRNLEDE RMRMIENASE VLITPLEKFR 130 140 150 160 170 180 KEQIGAAKDA KKKYDKETEK YCGVLEKHLN LSSKKKESQL QEADSQVDLV RQHFYEVSLE 190 200 210 220 230 240 YVFKVQEVQE RKMFEFVEPL LAFLQGLFTF YHHGYELAKD FSDFKTELTI SIQNTRNRFE 250 260 270 280 290 300 GTRSEVESLM KKMKENPHEH KNISPYTMEG YLYVQEKRHF GTSWVKHYCT YQRESKRITM 310 320 330 340 350 360 VPFDQKSGGK GGEDEAVILK SCTRRKTDSI EKRFCFDVEA VDRPGVITMQ ALSEEDRRLW 370 380 390 400 410 420 MEAMDGREPV YNSNKDNQSE GTAQLDSIGF SIIKKCIHAV ETRGINEQGL YRIVGVNSRV 430 440 450 460 470 480 QKLLSILMDP KTATETETEI CAEWEIKTIT SALKTYLRML PGPLMMYQFQ RSFIKAAKLE 490 500 510 520 530 540 NQESRVSEIH SLVHRLPEKN RQMLHLLMNH LAKVADNHKQ NLMTVANLGV VFGPTLLRPQ 550 560 570 580 590 600 EETVAAIMDI KFQNIVIEIL IENHEKIFNT VPETPPSNSQ LLLSRKKSTD SKPPSCSERP 610 620 630 640 650 660 LTLFHTAQPN EKQESRNSII NSSLESVISS NANSFLNSNS APQSNLNSSD LELEVIKPNR 670 680 690 700 710 720 PNSLPQNPSP TSPLSPSWPM FSAPSSPMPT SSTSSDSSPI SSPLRKARAL YACKAEHDSE 730 740 750 760 LSFTAGTVFD NVHPSQEPGW LEGTLNGKTG LIPENYVEFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.
[2]	"An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinase." Hildebrand J.D., Taylor J.M., Parsons J.T. Mol. Cell. Biol. 16:3169-3178(1996) [PubMed: 8649427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-760, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PTK2/FAK, TISSUE SPECIFICITY. Tissue: Embryo.
[3]	"Structure of the BH domain from Graf and its implications for Rho GTPase recognition." Longenecker K.L., Zhang B., Derewenda U., Sheffield P.J., Dauter Z., Parsons J.T., Zheng Y., Derewenda Z.S. J. Biol. Chem. 275:38605-38610(2000) [PubMed: 10982819] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 161-391, FUNCTION.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AJ719269 mRNA. Translation: CAG30928.1.
U36309 mRNA. Translation: AAB07998.1. Different initiation.

IPI

IPI00596193.

RefSeq

NP_990525.1.

UniGene

Gga.2901

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1F7C	X-ray	2.40	A	353-583	[»]

SMR

Q5ZMW5. Positions 695-760.

ModBase

Search...

Genome annotation databases

Ensembl

ENSGALG00000007380. Gallus gallus. [Contig view]

GeneID

396113.

KEGG

gga:396113.

Phylogenomic databases

HOVERGEN

Q5ZMW5.

Family and domain databases

InterPro

IPR011993. PH_type.
IPR001849. Pleckstrin_homology.
IPR000198. RhoGAP.
IPR001452. SH3_domain.
[Graphical view]

Gene3D

G3DSA:2.30.29.30. PH_type. 1 hit.
G3DSA:1.10.555.10. RhoGAP. 1 hit.

Pfam

PF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

ProDom

PD000066. SH3. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]

PROSITE

PS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RHG26_CHICK

Accession

Primary (citable) accession number: Q5ZMW5
Secondary accession number(s): Q98935

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	June 16, 2009
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)