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Protein

DNA replication licensing factor MCM3

Gene

MCM3

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi346 – 353ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: AgBase
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 (EC:3.6.4.12)
Gene namesi
Name:MCM3
ORF Names:RCJMB04_1j4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  • MCM complex Source: UniProtKB
  • nucleus Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003189041 – 812DNA replication licensing factor MCM3Add BLAST812

Proteomic databases

PaxDbiQ5ZMN2.
PRIDEiQ5ZMN2.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Associated with the replication-specific DNA polymerase alpha (By similarity).By similarity

Protein-protein interaction databases

BioGridi682782. 1 interactor.
STRINGi9031.ENSGALP00000026858.

Structurei

3D structure databases

ProteinModelPortaliQ5ZMN2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini296 – 503MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi478 – 481Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224126.
HOVERGENiHBG104962.
InParanoidiQ5ZMN2.
KOiK02541.
PhylomeDBiQ5ZMN2.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZMN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPAGGLGD AELREAQRDY LDFLDDEEDQ GVYHGKVRDM ISDNQYRLLV
60 70 80 90 100
NINDLRRRNE KRANRLLSNA FEELIAFQRA LKDFVASVDA TYAKQYEDFY
110 120 130 140 150
IGLEGSFGSK HVSPRTLTAC FLSCIVCVEG IVTKCSLIRP KVVRSVHYCP
160 170 180 190 200
ATKKTIERRY TDLTSLDAFP SSTVYPTKDE ENNPLETEYG LSVYKDHQTI
210 220 230 240 250
SIQEMPEKAP AGQLPRSVDV ILDDDLVDKV KPGDRIQVVG TYRCLPGKKG
260 270 280 290 300
GYTSGTFRTI LIACHIKQMS KDARPLYSAN DVAKIKRFSK SRSKDIFNQL
310 320 330 340 350
ARSLAPSIHG HEFIKKALLC MLLGGVEKVL ENGSRIRGDI NILLIGDPSV
360 370 380 390 400
AKSQLLRYVL GTAPRAVGTT GRGSSGVGLT AAVTTDQETG ERRLEAGAMV
410 420 430 440 450
LADRGVVCID EFDKMSDIDR TAIHEVMEQG RVTIAKAGIH ARLNSRCSVL
460 470 480 490 500
AAANPVYGRY DQYKTPMENI GLQDSLLSRF DLLFIMLDQM DSEQDREISD
510 520 530 540 550
HVLRMHRYRN PNEQDGDAMP LGSAVEILAT DDPDFAQEEE QELQVYEKHD
560 570 580 590 600
DLLHGPNRRK EKIVSMEFMR KYIHVAKMIK PVLTQESADY IAEEYSSLRS
610 620 630 640 650
QNQMNSDIAR TSPVTARTLE TLIRLSTAHA KARMSKTVDL QDAEAALELV
660 670 680 690 700
QFAYFKKVLE KEKKRKKQVE DDSETEKEEE EEETQPEKEG RKQRRKKART
710 720 730 740 750
EGEEESYDPY DFSDAEQEMP EVQAHTPKTP ESSATGEAKK PELADPRLKA
760 770 780 790 800
FKAALLEVFK SSHAQSVGLK NVMESINRDN PEPFSLAGVK VALAHMQDDN
810
QIMVSDDIIF LI
Length:812
Mass (Da):91,251
Last modified:November 23, 2004 - v1
Checksum:i5D65A2F86936E234
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719352 mRNA. Translation: CAG31011.1.
RefSeqiNP_001006421.1. NM_001006421.1.
UniGeneiGga.4920.

Genome annotation databases

GeneIDi422043.
KEGGigga:422043.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719352 mRNA. Translation: CAG31011.1.
RefSeqiNP_001006421.1. NM_001006421.1.
UniGeneiGga.4920.

3D structure databases

ProteinModelPortaliQ5ZMN2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi682782. 1 interactor.
STRINGi9031.ENSGALP00000026858.

Proteomic databases

PaxDbiQ5ZMN2.
PRIDEiQ5ZMN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi422043.
KEGGigga:422043.

Organism-specific databases

CTDi4172.

Phylogenomic databases

eggNOGiKOG0479. Eukaryota.
COG1241. LUCA.
HOGENOMiHOG000224126.
HOVERGENiHBG104962.
InParanoidiQ5ZMN2.
KOiK02541.
PhylomeDBiQ5ZMN2.

Miscellaneous databases

PROiQ5ZMN2.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
PF17207. MCM_OB. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCM3_CHICK
AccessioniPrimary (citable) accession number: Q5ZMN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 23, 2004
Last modified: November 30, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.