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Protein

Malate dehydrogenase, cytoplasmic

Gene

MDH1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921SubstrateBy similarity
Binding sitei98 – 981SubstrateBy similarity
Binding sitei105 – 1051NADBy similarity
Binding sitei112 – 1121NADBy similarity
Binding sitei131 – 1311SubstrateBy similarity
Binding sitei162 – 1621SubstrateBy similarity
Active sitei187 – 1871Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 177NADBy similarity
Nucleotide bindingi129 – 1313NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: Reactome
  2. cellular carbohydrate metabolic process Source: InterPro
  3. gluconeogenesis Source: Reactome
  4. malate metabolic process Source: InterPro
  5. small molecule metabolic process Source: Reactome
  6. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_115534. Gluconeogenesis.
REACT_352557. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Alternative name(s):
Cytosolic malate dehydrogenase
Gene namesi
Name:MDH1
ORF Names:RCJMB04_2g5
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Chromosome 3

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. centrosome Source: Ensembl
  2. extracellular space Source: Ensembl
  3. extracellular vesicular exosome Source: Ensembl
  4. mitochondrion Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Malate dehydrogenase, cytoplasmicPRO_0000226738Add
BLAST

Proteomic databases

PaxDbiQ5ZME2.
PRIDEiQ5ZME2.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi682121. 1 interaction.
IntActiQ5ZME2. 1 interaction.
STRINGi9031.ENSGALP00000014374.

Structurei

3D structure databases

ProteinModelPortaliQ5ZME2.
SMRiQ5ZME2. Positions 3-334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00530000063410.
HOGENOMiHOG000220953.
HOVERGENiHBG006340.
InParanoidiQ5ZME2.
KOiK00025.
OMAiFVTTVQQ.
OrthoDBiEOG78H3TM.
PhylomeDBiQ5ZME2.
TreeFamiTF105826.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZME2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEPIRVLVT GAAGQIAYSL LYSIAKGDVF GKEQPLVLVL LDITPMMTVL
60 70 80 90 100
EGVVMELQDC ALPLLREVIP TDKEEVAFKD LDIAILVGSM PRREGMERKD
110 120 130 140 150
LLKANVKIFK SQGAALDKYA KKTVKVVVVG NPANTNCLIA SKSAPSIPKE
160 170 180 190 200
NFSCLTRLDH NRAKSQIALK LGVTSNDVKN VIIWGNHSST QYPDVNHAKV
210 220 230 240 250
NVKGKEVGVY EAIKDDSWLK GDFILTVQQR GAAVIKARKL SSAMSAAKAI
260 270 280 290 300
CDHVRDIWFG TPAGEFVSMG VISDGNSYGV PEDLLYSFPV VIKDKTWKFV
310 320 330
EGLPINDFSR EKMDLTAKEL TEEKETAVEF LSSA
Length:334
Mass (Da):36,543
Last modified:November 23, 2004 - v1
Checksum:i2AD5D831DC812332
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719442 mRNA. Translation: CAG31101.1.
RefSeqiNP_001006395.1. NM_001006395.1.
UniGeneiGga.1141.

Genome annotation databases

EnsembliENSGALT00000014390; ENSGALP00000014374; ENSGALG00000008858.
GeneIDi421281.
KEGGigga:421281.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719442 mRNA. Translation: CAG31101.1.
RefSeqiNP_001006395.1. NM_001006395.1.
UniGeneiGga.1141.

3D structure databases

ProteinModelPortaliQ5ZME2.
SMRiQ5ZME2. Positions 3-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi682121. 1 interaction.
IntActiQ5ZME2. 1 interaction.
STRINGi9031.ENSGALP00000014374.

Proteomic databases

PaxDbiQ5ZME2.
PRIDEiQ5ZME2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000014390; ENSGALP00000014374; ENSGALG00000008858.
GeneIDi421281.
KEGGigga:421281.

Organism-specific databases

CTDi4190.

Phylogenomic databases

eggNOGiCOG0039.
GeneTreeiENSGT00530000063410.
HOGENOMiHOG000220953.
HOVERGENiHBG006340.
InParanoidiQ5ZME2.
KOiK00025.
OMAiFVTTVQQ.
OrthoDBiEOG78H3TM.
PhylomeDBiQ5ZME2.
TreeFamiTF105826.

Enzyme and pathway databases

ReactomeiREACT_115534. Gluconeogenesis.
REACT_352557. Gluconeogenesis.

Miscellaneous databases

NextBioi20824070.
PROiQ5ZME2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiMDHC_CHICK
AccessioniPrimary (citable) accession number: Q5ZME2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: November 23, 2004
Last modified: April 1, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.