Q5ZME2 (MDHC_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase, cytoplasmic
EC=1.1.1.37

Alternative name(s):
Cytosolic malate dehydrogenase

Gene names

Name:	MDH1
ORF Names:	RCJMB04_2g5

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro gluconeogenesis Traceable author statement. Source: Reactome malate metabolic process Inferred from electronic annotation. Source: InterPro small molecule metabolic process Traceable author statement. Source: Reactome tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	centrosome Inferred from electronic annotation. Source: Compara mitochondrion Inferred from electronic annotation. Source: Compara
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Malate dehydrogenase, cytoplasmic		PRO_0000226738
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
	Nucleotide binding	129 – 131	3	NAD By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	105	1	NAD By similarity
	Binding site	112	1	NAD By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZME2 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 2AD5D831DC812332	FASTA	334	36,543
	10 20 30 40 50 60 MGEPIRVLVT GAAGQIAYSL LYSIAKGDVF GKEQPLVLVL LDITPMMTVL EGVVMELQDC 70 80 90 100 110 120 ALPLLREVIP TDKEEVAFKD LDIAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA 130 140 150 160 170 180 KKTVKVVVVG NPANTNCLIA SKSAPSIPKE NFSCLTRLDH NRAKSQIALK LGVTSNDVKN 190 200 210 220 230 240 VIIWGNHSST QYPDVNHAKV NVKGKEVGVY EAIKDDSWLK GDFILTVQQR GAAVIKARKL 250 260 270 280 290 300 SSAMSAAKAI CDHVRDIWFG TPAGEFVSMG VISDGNSYGV PEDLLYSFPV VIKDKTWKFV 310 320 330 EGLPINDFSR EKMDLTAKEL TEEKETAVEF LSSA

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ719442 mRNA. Translation: CAG31101.1.
IPI	IPI00583336.
RefSeq	NP_001006395.1. NM_001006395.1.
UniGene	Gga.1141.
3D structure databases
ProteinModelPortal	Q5ZME2.
SMR	Q5ZME2. Positions 3-334.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5ZME2. 1 interaction.
STRING	9031.ENSGALP00000014374.
Proteomic databases
PaxDb	Q5ZME2.
PRIDE	Q5ZME2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000014390; ENSGALP00000014374; ENSGALG00000008858.
GeneID	421281.
KEGG	gga:421281.
Organism-specific databases
CTD	4190.
Phylogenomic databases
eggNOG	COG0039.
GeneTree	ENSGT00530000063410.
HOGENOM	HOG000220953.
HOVERGEN	HBG006340.
InParanoid	Q5ZME2.
KO	K00025.
OMA	NCLIASK.
OrthoDB	EOG4CVG78.
Enzyme and pathway databases
Reactome	REACT_115655. Metabolism.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR011274. Malate_DH_NAD-dep_euk. IPR010945. Malate_DH_type2. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR23382. PTHR23382. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit. TIGR01758. MDH_euk_cyt. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20824070.

Entry information

Entry name

MDHC_CHICK

Accession

Primary (citable) accession number: Q5ZME2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program