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Q5ZME2 (MDHC_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase, cytoplasmic

EC=1.1.1.37
Alternative name(s):
Cytosolic malate dehydrogenase
Gene names
Name:MDH1
ORF Names:RCJMB04_2g5
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01517

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01517.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Malate dehydrogenase, cytoplasmic HAMAP-Rule MF_01517
PRO_0000226738

Regions

Nucleotide binding11 – 177NAD By similarity
Nucleotide binding129 – 1313NAD By similarity

Sites

Active site1871Proton acceptor By similarity
Binding site921Substrate By similarity
Binding site981Substrate By similarity
Binding site1051NAD By similarity
Binding site1121NAD By similarity
Binding site1311Substrate By similarity
Binding site1621Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5ZME2 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 2AD5D831DC812332

FASTA33436,543
        10         20         30         40         50         60 
MGEPIRVLVT GAAGQIAYSL LYSIAKGDVF GKEQPLVLVL LDITPMMTVL EGVVMELQDC 

        70         80         90        100        110        120 
ALPLLREVIP TDKEEVAFKD LDIAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA 

       130        140        150        160        170        180 
KKTVKVVVVG NPANTNCLIA SKSAPSIPKE NFSCLTRLDH NRAKSQIALK LGVTSNDVKN 

       190        200        210        220        230        240 
VIIWGNHSST QYPDVNHAKV NVKGKEVGVY EAIKDDSWLK GDFILTVQQR GAAVIKARKL 

       250        260        270        280        290        300 
SSAMSAAKAI CDHVRDIWFG TPAGEFVSMG VISDGNSYGV PEDLLYSFPV VIKDKTWKFV 

       310        320        330 
EGLPINDFSR EKMDLTAKEL TEEKETAVEF LSSA 

« Hide

References

[1]"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis."
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.
Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: CB.
Tissue: Bursa of Fabricius.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ719442 mRNA. Translation: CAG31101.1.
RefSeqNP_001006395.1. NM_001006395.1.
UniGeneGga.1141.

3D structure databases

ProteinModelPortalQ5ZME2.
SMRQ5ZME2. Positions 3-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid682121. 1 interaction.
IntActQ5ZME2. 1 interaction.
STRING9031.ENSGALP00000014374.

Proteomic databases

PaxDbQ5ZME2.
PRIDEQ5ZME2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSGALT00000014390; ENSGALP00000014374; ENSGALG00000008858.
GeneID421281.
KEGGgga:421281.

Organism-specific databases

CTD4190.

Phylogenomic databases

eggNOGCOG0039.
GeneTreeENSGT00530000063410.
HOGENOMHOG000220953.
HOVERGENHBG006340.
InParanoidQ5ZME2.
KOK00025.
OMANCLIASK.
OrthoDBEOG78H3TM.
PhylomeDBQ5ZME2.
TreeFamTF105826.

Enzyme and pathway databases

ReactomeREACT_115655. Metabolism.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01517. Malate_dehydrog_2.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20824070.
PROQ5ZME2.

Entry information

Entry nameMDHC_CHICK
AccessionPrimary (citable) accession number: Q5ZME2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families