Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histone H2A.Z

Gene

H2AFZ

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Histone H2A.Z
Short name:
H2A/z
Gene namesi
Name:H2AFZ
ORF Names:RCJMB04_2h11
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 4

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleosome core, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 128127Histone H2A.ZPRO_0000055302Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51N6-acetyllysine1 Publication
Modified residuei5 – 51N6-methyllysineBy similarity
Modified residuei8 – 81N6-acetyllysine1 Publication
Modified residuei8 – 81N6-methyllysineBy similarity
Modified residuei12 – 121N6-acetyllysine1 Publication
Cross-linki122 – 122Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.By similarity
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis (By similarity).By similarity
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Ubl conjugation

Proteomic databases

PaxDbiQ5ZMD6.
PRIDEiQ5ZMD6.

Interactioni

Subunit structurei

The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. H2A or its variant H2AFZ forms a heterodimer with H2B (By similarity).By similarity

Protein-protein interaction databases

BioGridi686040. 1 interaction.
STRINGi9031.ENSGALP00000028277.

Structurei

3D structure databases

ProteinModelPortaliQ5ZMD6.
SMRiQ5ZMD6. Positions 17-123.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni89 – 10012M6 cassetteBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the histone H2A family.Curated

Phylogenomic databases

eggNOGiKOG1757. Eukaryota.
COG5262. LUCA.
GeneTreeiENSGT00710000106735.
HOVERGENiHBG009342.
InParanoidiQ5ZMD6.
KOiK11251.
OMAiCAAILEY.
OrthoDBiEOG7M0NTR.
PhylomeDBiQ5ZMD6.
TreeFamiTF354232.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR002119. Histone_H2A.
IPR007125. Histone_H2A/H2B/H3.
IPR032454. Histone_H2A_C.
IPR032458. Histone_H2A_CS.
[Graphical view]
PfamiPF00125. Histone. 1 hit.
PF16211. Histone_H2A_C. 1 hit.
[Graphical view]
PRINTSiPR00620. HISTONEH2A.
SMARTiSM00414. H2A. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00046. HISTONE_H2A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5ZMD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGGKAGKDS GKTKTKAVSR SQRAGLQFPV GRIHRHLKSR TTSHGRVGAT
60 70 80 90 100
AAVYSAAILE YLTAEVLELA GNASKDLKVK RITPRHLQLA IRGDEELDSL
110 120
IKATIAGGGV IPHIHKSLIG KKGQQKTV
Length:128
Mass (Da):13,583
Last modified:January 23, 2007 - v3
Checksum:iE031E537B2930371
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719448 mRNA. Translation: CAG31107.1.
RefSeqiNP_001026545.1. NM_001031374.1.
UniGeneiGga.4671.

Genome annotation databases

EnsembliENSGALT00000028340; ENSGALP00000028277; ENSGALG00000014023.
GeneIDi426361.
KEGGigga:426361.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719448 mRNA. Translation: CAG31107.1.
RefSeqiNP_001026545.1. NM_001031374.1.
UniGeneiGga.4671.

3D structure databases

ProteinModelPortaliQ5ZMD6.
SMRiQ5ZMD6. Positions 17-123.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi686040. 1 interaction.
STRINGi9031.ENSGALP00000028277.

Proteomic databases

PaxDbiQ5ZMD6.
PRIDEiQ5ZMD6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000028340; ENSGALP00000028277; ENSGALG00000014023.
GeneIDi426361.
KEGGigga:426361.

Organism-specific databases

CTDi3015.

Phylogenomic databases

eggNOGiKOG1757. Eukaryota.
COG5262. LUCA.
GeneTreeiENSGT00710000106735.
HOVERGENiHBG009342.
InParanoidiQ5ZMD6.
KOiK11251.
OMAiCAAILEY.
OrthoDBiEOG7M0NTR.
PhylomeDBiQ5ZMD6.
TreeFamiTF354232.

Miscellaneous databases

NextBioi20827985.
PROiQ5ZMD6.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR002119. Histone_H2A.
IPR007125. Histone_H2A/H2B/H3.
IPR032454. Histone_H2A_C.
IPR032458. Histone_H2A_CS.
[Graphical view]
PfamiPF00125. Histone. 1 hit.
PF16211. Histone_H2A_C. 1 hit.
[Graphical view]
PRINTSiPR00620. HISTONEH2A.
SMARTiSM00414. H2A. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS00046. HISTONE_H2A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.
  2. "Effects of histone acetylation, ubiquitination and variants on nucleosome stability."
    Li W., Nagaraja S., Delcuve G.P., Hendzel M.J., Davie J.R.
    Biochem. J. 296:737-744(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION.
  3. "The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken."
    Bruce K., Myers F.A., Mantouvalou E., Lefevre P., Greaves I., Bonifer C., Tremethick D.J., Thorne A.W., Crane-Robinson C.
    Nucleic Acids Res. 33:5633-5639(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT LYS-5; LYS-8 AND LYS-12.
  4. "Protein identification using sequential ion/ion reactions and tandem mass spectrometry."
    Coon J.J., Ueberheide B., Syka J.E.P., Dryhurst D.D., Ausio J., Shabanowitz J., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 102:9463-9468(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiH2AZ_CHICK
AccessioniPrimary (citable) accession number: Q5ZMD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: January 23, 2007
Last modified: February 17, 2016
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.