Q5ZMA6 (NADE_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine-dependent NAD(+) synthetase
EC=6.3.5.1

Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]
NAD(+) synthetase

Gene names

Name:	NADSYN1
ORF Names:	RCJMB04_2l1

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1.
Sequence similarities	In the C-terminal section; belongs to the NAD synthetase family. Contains 1 CN hydrolase domain.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	Glutamine-dependent NAD(+) synthetase		PRO_0000237581
Regions
	Domain	5 – 294	290	CN hydrolase
	Nucleotide binding	355 – 362	8	ATP By similarity
	Region	325 – 706	382	Ligase By similarity
Sites
	Active site	357	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZMA6 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 8659E49307E3FCC6	FASTA	707	79,227
	10 20 30 40 50 60 MGRAVSVAAC ALNQWALDFE GNAERILRSI SIAKSKGARY RLGPELEICG YGCADHYYES 70 80 90 100 110 120 DTLLHSFQVL AKLLESPATQ DIICDVGMPL MHRNVRYNCR VIFLNKKILL IRPKISLANA 130 140 150 160 170 180 GNYRELRWFT PWNKARHVEE YLLPRIIQEV TGQDTVPFGD AVLATKDTCL GTEICEELWA 190 200 210 220 230 240 PNSPHIEMGL DGVEIFTNSS GSHHVLRKAH TRVDLVNSAT AKNGGIYILS NQKGCDGDRL 250 260 270 280 290 300 YYDGCAMISM NGETVAQGSQ FSLDDVEVLV ATLDLEDVRS YRAEISSRNL AASKVNPFPR 310 320 330 340 350 360 IKVNFALSCS DDLSVPICVP IQWRHHSPEE EICLGPACWL WDYLRRSKQA GFLLPLSGGI 370 380 390 400 410 420 DSSATACIVY SMCRQVCLAV KNGNSEVLAD ARKIVHDETY IPEDPQEFCK RVFTTCYMAS 430 440 450 460 470 480 ENSSQDTRNR AKLLAEQIGS YHINLNIDAA VKAIVGIFSM VTGRTPRFSV YGGSRRENLA 490 500 510 520 530 540 LQNVQARVRM VPAYLFAQLT LWTRGMPGGL LVLGSANVDE SLRGYLTKYD CSSADINPIG 550 560 570 580 590 600 GISKTDLKNF IQYCIENFQL TALRSIMAAP PTAELEPLMD GQVSQTDEAD MGMTYAELSI 610 620 630 640 650 660 YGKLRKIAKA GPYSMFCKLI NLWKEICTPR EVASKVKHFF RMYSVNRHKM TTLTPSYHAE 670 680 690 700 NYSPDDNRFD LRPFLYNTTW SWQFRCIDNQ VSHLEKKEGI SVAEDTD

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ719478 mRNA. Translation: CAG31137.1.
IPI	IPI00595403.
RefSeq	NP_001006465.1. NM_001006465.1.
UniGene	Gga.22360.
3D structure databases
ProteinModelPortal	Q5ZMA6.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000006591.
Proteomic databases
PaxDb	Q5ZMA6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	422983.
KEGG	gga:422983.
Organism-specific databases
CTD	55191.
Phylogenomic databases
eggNOG	COG0388.
HOVERGEN	HBG082007.
InParanoid	Q5ZMA6.
KO	K01950.
OrthoDB	EOG45X7VK.
Enzyme and pathway databases
UniPathway	UPA00253; UER00334.
Family and domain databases
Gene3D	3.40.50.620. 1 hit. 3.60.110.10. 1 hit.
InterPro	IPR003010. C-N_Hydrolase. IPR014445. Gln-dep_NAD_synthase. IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Pfam	PF00795. CN_hydrolase. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF006630. NADS_GAT. 1 hit.
SUPFAM	SSF56317. Ntlse/CNhydtse. 1 hit.
TIGRFAMs	TIGR00552. nadE. 1 hit.
PROSITE	PS50263. CN_HYDROLASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20825523.

Entry information

Entry name

NADE_CHICK

Accession

Primary (citable) accession number: Q5ZMA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program