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Protein

cAMP-dependent protein kinase type I-alpha regulatory subunit

Gene

PRKAR1A

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei203 – 2031cAMP 1By similarity
Binding sitei212 – 2121cAMP 1By similarity
Binding sitei327 – 3271cAMP 2By similarity
Binding sitei336 – 3361cAMP 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi138 – 255118cAMP 1Add
BLAST
Nucleotide bindingi256 – 382127cAMP 2Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-163615. PKA activation.
R-GGA-164378. PKA activation in glucagon signalling.
R-GGA-180024. DARPP-32 events.
R-GGA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-GGA-5610787. Hedgehog 'off' state.
R-GGA-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type I-alpha regulatory subunit
Gene namesi
Name:PRKAR1A
ORF Names:RCJMB04_2n5
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 18

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 382381cAMP-dependent protein kinase type I-alpha regulatory subunitPRO_0000293483Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Disulfide bondi18 – 18Interchain (with C-39)By similarity
Disulfide bondi39 – 39Interchain (with C-18)By similarity

Post-translational modificationi

The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain but is not phosphorylated. The physiological significance of phosphorylations by other kinases is unclear (By similarity).By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ5ZM91.
PRIDEiQ5ZM91.

Interactioni

Subunit structurei

The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules (By similarity).By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006731.

Structurei

3D structure databases

ProteinModelPortaliQ5ZM91.
SMRiQ5ZM91. Positions 14-63, 93-382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 136135Dimerization and phosphorylationSequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi97 – 1015Pseudophosphorylation motif

Sequence similaritiesi

Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1113. Eukaryota.
COG0664. LUCA.
GeneTreeiENSGT00530000062947.
HOGENOMiHOG000196669.
HOVERGENiHBG002025.
InParanoidiQ5ZM91.
KOiK04739.
OMAiQTMPESE.
OrthoDBiEOG72JWG6.
PhylomeDBiQ5ZM91.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view]
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
SMARTiSM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view]
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5ZM91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSSSSSSE EERSLRECEL YVQKHNIQQL LKDCIVQLCT VRPDRPMGFL
60 70 80 90 100
REYFERLEKE ETKQLLNQQK SGSRSDSRED EISPPPPMNP VVKGRRRRGA
110 120 130 140 150
ISAEVYTEED AASYVRKVIP KDYKTMAALA KAIEKNVLFA HLDDNERSDI
160 170 180 190 200
FDAMFPVTYI AGETVIQQGD EGDNFYVVDQ GEMDVYVNNE WATSVGEGGS
210 220 230 240 250
FGELALIYGT PRAATVKAKT NVKLWGIDRD SYRRILMGST LRKRKMYEEF
260 270 280 290 300
LSKVSILESL DKWERLTVAD ALEPVQFEDG QKIVVQGEPG DEFFIILEGT
310 320 330 340 350
AAVLQRRSEN EEFVEVGRLA PSDYFGEIAL LMNRPRAATV VARGLLKCVK
360 370 380
LDRPRFERVL GPCSDILKRN IQQYNSFVSL SV
Length:382
Mass (Da):43,350
Last modified:November 23, 2004 - v1
Checksum:iB7F778C55FC866D8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719493 mRNA. Translation: CAG31152.1.
RefSeqiNP_001007846.1. NM_001007845.1.
XP_015135378.1. XM_015279892.1.
XP_015135379.1. XM_015279893.1.
XP_015135380.1. XM_015279894.1.
UniGeneiGga.4713.

Genome annotation databases

EnsembliENSGALT00000034558; ENSGALP00000033915; ENSGALG00000004237.
GeneIDi417438.
KEGGigga:417438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719493 mRNA. Translation: CAG31152.1.
RefSeqiNP_001007846.1. NM_001007845.1.
XP_015135378.1. XM_015279892.1.
XP_015135379.1. XM_015279893.1.
XP_015135380.1. XM_015279894.1.
UniGeneiGga.4713.

3D structure databases

ProteinModelPortaliQ5ZM91.
SMRiQ5ZM91. Positions 14-63, 93-382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006731.

Proteomic databases

PaxDbiQ5ZM91.
PRIDEiQ5ZM91.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000034558; ENSGALP00000033915; ENSGALG00000004237.
GeneIDi417438.
KEGGigga:417438.

Organism-specific databases

CTDi5573.

Phylogenomic databases

eggNOGiKOG1113. Eukaryota.
COG0664. LUCA.
GeneTreeiENSGT00530000062947.
HOGENOMiHOG000196669.
HOVERGENiHBG002025.
InParanoidiQ5ZM91.
KOiK04739.
OMAiQTMPESE.
OrthoDBiEOG72JWG6.
PhylomeDBiQ5ZM91.

Enzyme and pathway databases

ReactomeiR-GGA-163615. PKA activation.
R-GGA-164378. PKA activation in glucagon signalling.
R-GGA-180024. DARPP-32 events.
R-GGA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-GGA-5610787. Hedgehog 'off' state.
R-GGA-983231. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

PROiQ5ZM91.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view]
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
SMARTiSM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view]
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiKAP0_CHICK
AccessioniPrimary (citable) accession number: Q5ZM91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: November 23, 2004
Last modified: June 8, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.