Q5ZM45 (UBP48_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 48
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 48
Ubiquitin thioesterase 48
Ubiquitin-specific-processing protease 48

Gene names

Name:	USP48
ORF Names:	RCJMB04_3d5

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	1033 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase C19 family. Contains 3 DUSP domains. Contains 1 ubiquitin-like domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Domain	Repeat
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1033	1033	Ubiquitin carboxyl-terminal hydrolase 48		PRO_0000249526
Regions
	Domain	457 – 552	96	DUSP 1
	Domain	567 – 690	124	DUSP 2
	Domain	710 – 823	114	DUSP 3
	Domain	931 – 1007	77	Ubiquitin-like
Sites
	Active site	98	1	Nucleophile By similarity
	Active site	351	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZM45 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 55E1960AAFB983C2	FASTA	1,033	118,691
	10 20 30 40 50 60 MAPRLQLEKA AWRWTETVPP EAVTQEHIEA AYRVGLEPCQ RGTCRRNCRG NPNCLVGIGE 70 80 90 100 110 120 HVWLGEIDEN SFHNIDDPNC ERRKKNAFVG LTNLGATCYV NTFLQMWFLN LELRQALYLC 130 140 150 160 170 180 SSNEHAAGES IPKDKDYEPQ TICEHLQYLF ALLQNSKRRY IDPSGFVKAL GLDTGQQQDA 190 200 210 220 230 240 QEFSKLFMSL LEDTLSKQKN PDVRNIVQKQ FCGEYAYVTV CNQCGRESKL VSKFYELELN 250 260 270 280 290 300 IQGHKQLTDC ITEFLKEEKL EGDNRYFCET CQSKQNATRK IRLLSLPCTL NLQLMRFVFD 310 320 330 340 350 360 RQTGHKKKLN TYIGFSELLD MEPFMEQKNG VYVYELSAVL IHRGVSAYSG HYIAHVKDPQ 370 380 390 400 410 420 TGEWYKFNDE DIEKMEGKKL QLGIEEDLAE PSKSQTRKPK CGKGTHCSRN AYMLVYRLQT 430 440 450 460 470 480 REKSLTVEVP AFLQELVERD NCKFEEWCNE MAEMRKQSVA RGKIKHEEVK ELYQRLPAEA 490 500 510 520 530 540 GSPYDFISLE WLQKWLDEST PPKPIDNTAC LCSHGKLHPD KICIMKRISE YVADFFYRRY 550 560 570 580 590 600 GGGPRLNVKA LCKDCVVERC RILRLKNQLN EDYKTVTNLL KITVKGSEGF WVGKASLRSW 610 620 630 640 650 660 RQLALEQLNE QDEDAEHSNG KLNGNAPNKD EVNEEKREEE EELNFNEDIV CPHGDLCISE 670 680 690 700 710 720 NERRVVSKEA WKKLKQYFPK APEFPSNKEC CSQCKILERE GEENEALHKM MASEQKTSLQ 730 740 750 760 770 780 NLFHDKCRPC LGSWPQETDE LYIVSQFFVE EWRKFVRRPT RCSPVSSLGN SALLCPHGGL 790 800 810 820 830 840 MFTYASMTKE DSKLIALIWP SEWERIQKLF VVDHVIKITR TRAAGEPESA LYSSEPQLCP 850 860 870 880 890 900 ECRGGLLCQQ QRDLREYTQA TIYIHKVVDN KKVMKDAAPE LNVSSSEAEE EREENKPEGE 910 920 930 940 950 960 QDPDFNQSNG GAKRQKLSHQ SYISYQKQGI RRSTRHRKVR GEKALLVSAN QTLKELKIQI 970 980 990 1000 1010 1020 MHAFSVAPFD QNLLIDGKIL SDDTATLGSL GIIPESVILL KADEPIADYA AMDDVMQVCM 1030 PEEGFKGTGL LGH

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ719539 mRNA. Translation: CAG31198.1.
IPI	IPI00571210.
RefSeq	NP_001073211.1. NM_001079743.1.
UniGene	Gga.20232.
3D structure databases
HSSP	HSSP built from PDB template 2AYN based on UniProtKB P54578.
ProteinModelPortal	Q5ZM45.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000022836.
Protein family/group databases
MEROPS	C19.068.
Proteomic databases
PaxDb	Q5ZM45.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	769457.
KEGG	gga:769457.
Organism-specific databases
CTD	84196.
Phylogenomic databases
eggNOG	COG5077.
HOGENOM	HOG000231639.
HOVERGEN	HBG080368.
InParanoid	Q5ZM45.
KO	K11858.
OrthoDB	EOG4GF3DC.
Family and domain databases
InterPro	IPR006615. Pept_C19_DUSP. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR000626. Ubiquitin. IPR019955. Ubiquitin_supergroup. [Graphical view]
Pfam	PF00240. ubiquitin. 1 hit. PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00695. DUSP. 1 hit. SM00213. UBQ. 1 hit. [Graphical view]
PROSITE	PS51283. DUSP. 3 hits. PS00299. UBIQUITIN_1. False negative. PS50053. UBIQUITIN_2. 1 hit. PS00972. UCH_2_1. False negative. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20919686.

Entry information

Entry name

UBP48_CHICK

Accession

Primary (citable) accession number: Q5ZM45

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program