Q5ZM11 (SYRC_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase, cytoplasmic
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	RARS
ORF Names:	RCJMB04_3h11

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis By similarity.
Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).
Subunit structure	Monomer; also part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	The N-terminus (AA 1-72) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 661	661	Arginine--tRNA ligase, cytoplasmic		PRO_0000250729
Regions
	Region	1 – 72	72	Could be involved in the assembly of the multisynthetase complex By similarity
	Motif	202 – 213	12	"HIGH" region

Sequences

	Sequence		Length	Mass (Da)
	Q5ZM11 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 92D61B5BB5BC030B	FASTA	661	75,418
	10 20 30 40 50 60 MEARVAEAAA RLARQENEIK SLTAEIEHLK NFGCLGISPS LEGLRDENAK LKYRLNFLQK 70 80 90 100 110 120 SLQEEQSKTV KSMININSRL QEIFGAAIQA AYPELENPPL VVTPSQQPKF GDYQCNSAMG 130 140 150 160 170 180 ITQILLKTKE QKVSPREIAE KITKHIPANE CIEKVEIAGP GFINVHLRKD FVSKQLSSLL 190 200 210 220 230 240 VNGVQPPAIG KRKKVILDFS SPNIAKEMHV GHLRSTIIGE SMCRLFEFAG YDVLRLNHLG 250 260 270 280 290 300 DWGTQFGMLI AHLQDRFPDY LTVSPPIGDL QAFYKESKRR FDTEEEFKKR AYQCVVLLQS 310 320 330 340 350 360 KDPDFIKAWE LICDVSRKEF QKIYNCLDVT LTERGESFYQ DMMKDIVKEF EDKGFVQVDD 370 380 390 400 410 420 GRKIVFVPGF SVPLTIMKSD GGYTYDTSDL AALRHRLLEE KGDILIYVVD SGQSVHLQTV 430 440 450 460 470 480 FAAGQMIGWY DPKVTRITHA AFGVVLGEDK KKFKTRSGDT VRLMDLLEEG LKRAMDKLKD 490 500 510 520 530 540 KERDKVLTPE ELKAAQMSVA FGCIKYADLS HNRLNDYVFS FDKMLDDRGN TAAYLLYAFT 550 560 570 580 590 600 RIRAIARLAN IDEGMLRKAA REEVIVLDHE KEWKLGKCIL RFPEILQKIL DDLLLHTLCD 610 620 630 640 650 660 YLYELATTFT EFYDNCYCVE KDRQSGQIMK VNTWRLLLCE ATATIMAKGF DILGIKPVER M

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ719573 mRNA. Translation: CAG31232.1.
IPI	IPI00586083.
RefSeq	NP_001025778.1. NM_001030607.1.
UniGene	Gga.22550.
3D structure databases
ProteinModelPortal	Q5ZM11.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000002862.
Proteomic databases
PaxDb	Q5ZM11.
PRIDE	Q5ZM11.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	416168.
KEGG	gga:416168.
Organism-specific databases
CTD	5917.
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HOG000247212.
HOVERGEN	HBG029238.
InParanoid	Q5ZM11.
KO	K01887.
OrthoDB	EOG4D52X9.
Family and domain databases
Gene3D	3.30.1360.70. 1 hit. 3.40.50.620. 1 hit.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-ligase_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR11956. PTHR11956. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20819670.

Entry information

Entry name

SYRC_CHICK

Accession

Primary (citable) accession number: Q5ZM11

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program