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Protein

Eukaryotic translation initiation factor 2 subunit 1

Gene

EIF2S1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis, Translation regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-GGA-381042. PERK regulates gene expression.
R-GGA-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-GGA-72702. Ribosomal scanning and start codon recognition.
R-GGA-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit 1
Alternative name(s):
Eukaryotic translation initiation factor 2 subunit alpha
Short name:
eIF-2-alpha
Short name:
eIF-2A
Short name:
eIF-2alpha
Gene namesi
Name:EIF2S1
Synonyms:EIF2A
ORF Names:RCJMB04_4i21
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 5

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 315314Eukaryotic translation initiation factor 2 subunit 1PRO_0000331242Add
BLAST

Proteomic databases

PaxDbiQ5ZLX2.
PRIDEiQ5ZLX2.

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain. Component of the EIF2 complex (By similarity).By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000015560.

Structurei

3D structure databases

ProteinModelPortaliQ5ZLX2.
SMRiQ5ZLX2. Positions 4-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini17 – 8872S1 motifPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the eIF-2-alpha family.Curated
Contains 1 S1 motif domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2916. Eukaryota.
COG1093. LUCA.
GeneTreeiENSGT00390000007015.
HOGENOMiHOG000199476.
HOVERGENiHBG001910.
InParanoidiQ5ZLX2.
KOiK03237.
OMAiGHPYDAF.
OrthoDBiEOG7FZ012.
PhylomeDBiQ5ZLX2.
TreeFamiTF101502.

Family and domain databases

Gene3Di1.10.150.190. 1 hit.
2.40.50.140. 1 hit.
3.30.70.1130. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
IPR024055. TIF2_asu_C.
IPR024054. TIF2_asu_middle.
IPR011488. TIF_2_asu.
[Graphical view]
PfamiPF07541. EIF_2_alpha. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF110993. SSF110993. 1 hit.
SSF116742. SSF116742. 1 hit.
SSF50249. SSF50249. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5ZLX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPALSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE
60 70 80 90 100
LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED
110 120 130 140 150
KFTKSKTVYS ILRHVAEVLE YTKDEQLESL FQRTAWVFDD KYKRPGYGAY
160 170 180 190 200
DAFKHAVSDP AILDSLDLTE EERRVLIDNI NRRLTPQAVK IRADIEVACY
210 220 230 240 250
GYEGIDAVKE ALRAGLNCST ENMPIKINLI APPRYVMTTT TLERTEGLSV
260 270 280 290 300
LNQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQL ERLERENAEV
310
DGDDDAEEME AKTED
Length:315
Mass (Da):36,178
Last modified:April 29, 2008 - v2
Checksum:i7AD67C14FD92A491
GO

Sequence cautioni

The sequence CAG31271.1 differs from that shown. Reason: Erroneous termination at position 289. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti289 – 2891Missing in CAG31271 (PubMed:15642098).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BU385483 mRNA. No translation available.
AJ719612 mRNA. Translation: CAG31271.1. Sequence problems.
RefSeqiNP_001006477.1. NM_001006477.1.
UniGeneiGga.21991.

Genome annotation databases

EnsembliENSGALT00000015576; ENSGALP00000015560; ENSGALG00000009565.
GeneIDi423279.
KEGGigga:423279.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BU385483 mRNA. No translation available.
AJ719612 mRNA. Translation: CAG31271.1. Sequence problems.
RefSeqiNP_001006477.1. NM_001006477.1.
UniGeneiGga.21991.

3D structure databases

ProteinModelPortaliQ5ZLX2.
SMRiQ5ZLX2. Positions 4-303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000015560.

Proteomic databases

PaxDbiQ5ZLX2.
PRIDEiQ5ZLX2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000015576; ENSGALP00000015560; ENSGALG00000009565.
GeneIDi423279.
KEGGigga:423279.

Organism-specific databases

CTDi1965.

Phylogenomic databases

eggNOGiKOG2916. Eukaryota.
COG1093. LUCA.
GeneTreeiENSGT00390000007015.
HOGENOMiHOG000199476.
HOVERGENiHBG001910.
InParanoidiQ5ZLX2.
KOiK03237.
OMAiGHPYDAF.
OrthoDBiEOG7FZ012.
PhylomeDBiQ5ZLX2.
TreeFamiTF101502.

Enzyme and pathway databases

ReactomeiR-GGA-381042. PERK regulates gene expression.
R-GGA-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-GGA-72702. Ribosomal scanning and start codon recognition.
R-GGA-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiQ5ZLX2.

Family and domain databases

Gene3Di1.10.150.190. 1 hit.
2.40.50.140. 1 hit.
3.30.70.1130. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
IPR024055. TIF2_asu_C.
IPR024054. TIF2_asu_middle.
IPR011488. TIF_2_asu.
[Graphical view]
PfamiPF07541. EIF_2_alpha. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF110993. SSF110993. 1 hit.
SSF116742. SSF116742. 1 hit.
SSF50249. SSF50249. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: White leghorn.
    Tissue: Trunk.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiIF2A_CHICK
AccessioniPrimary (citable) accession number: Q5ZLX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: June 8, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.