ID   NSUN2_CHICK             Reviewed;         796 AA.
AC   Q5ZLV4;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   16-JUN-2009, entry version 29.
DE   RecName: Full=tRNA (cytosine-5-)-methyltransferase NSUN2;
DE            EC=2.1.1.29;
DE   AltName: Full=NOL1/NOP2/Sun domain family member 2;
GN   Name=NSUN2; ORFNames=RCJMB04_4l11;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archosauria; Dinosauria; Saurischia; Theropoda; Coelurosauria; Aves;
OC   Neognathae; Galliformes; Phasianidae; Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=CB; TISSUE=Bursa of Fabricius;
RX   PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA   Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J.,
RA   Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M.,
RA   Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.;
RT   "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT   function analysis.";
RL   Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005).
CC   -!- FUNCTION: RNA methyltransferase that methylates tRNAs, and
CC       possibly RNA polymerase III transcripts. Methylates cytosine to 5-
CC       methylcytosine (m5C) at position 34 of intron-containing
CC       tRNA(Leu)(CAA) precursors (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing 5-methylcytosine.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus (By similarity).
CC       Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RsmB/NOP
CC       family. TRM4 subfamily.
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DR   EMBL; AJ719630; CAG31289.1; -; mRNA.
DR   IPI; IPI00594887; -.
DR   RefSeq; NP_001026175.1; -.
DR   UniGene; Gga.20332; -.
DR   Ensembl; ENSGALG00000013064; Gallus gallus.
DR   GeneID; 420938; -.
DR   KEGG; gga:420938; -.
DR   HOGENOM; Q5ZLV4; -.
DR   HOVERGEN; Q5ZLV4; -.
DR   BRENDA; 2.1.1.29; 4.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0016428; F:tRNA (cytosine-5-)-methyltransferase activity; IEA:EC.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR001678; Fmu/NOL1/Nop2p.
DR   InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR   Pfam; PF01189; Nol1_Nop2_Fmu; 2.
DR   PROSITE; PS01153; NOL1_NOP2_SUN; FALSE_NEG.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Methyltransferase; Nucleus; Phosphoprotein; RNA-binding;
KW   S-adenosyl-L-methionine; Transferase; tRNA processing; tRNA-binding.
FT   CHAIN         1    796       tRNA (cytosine-5-)-methyltransferase
FT                                NSUN2.
FT                                /FTId=PRO_0000289225.
FT   REGION      184    190       S-adenosyl-L-methionine binding (By
FT                                similarity).
FT   ACT_SITE    321    321       Nucleophile (Potential).
FT   BINDING     215    215       S-adenosyl-L-methionine (By similarity).
FT   BINDING     242    242       S-adenosyl-L-methionine (By similarity).
FT   BINDING     268    268       S-adenosyl-L-methionine (By similarity).
SQ   SEQUENCE   796 AA;  90845 MW;  F2C352922B62D9AD CRC64;
     MGRRARDRRR QLQPQQRRER SGGGGGGGDD QAGWAGGYPE IVKENELFER YYREQRIVPD
     GEWDAFMAAL REPLPATLRI TGYKSHAREI LHCLKEKYFR ELQHLEVDGQ KVEMPQALSW
     YPEELAWHTN LSRKILRKSP QLERFHQFLV SETECGNISR QEAVSMIPPL LLNVNPDHKI
     LDMCAAPGSK TAQLIEMLHA DMNVPFPKGF VIANDVDNKR CYLLVHQAKR LNSPCIMVVN
     HDASSIPNLQ VDVDGRKETL FYDRILCDVP CSGDGTMRKN IDVWKKWTTQ NSLQLHGLQL
     RIATRGVEQL AEGGRMVYST CSLNPIENEA VIASLLEKSQ GALELADVSS ELPGLKRMPG
     ITKWKVMLKD GQWFEEWKDV PSNRQTQIRP TMFPIKEEEK LKAMNLERCI RILPHHQNTG
     GFFVAVLIKK SPMPWNKRQP KVHQKLPGKT EDTEVTATNA GDGSEDATEK PTLAEDEEPK
     KVQELQNSDT EQSKKGVCGP PPSKKMKLFG FKEDPFVFLP EDDPLFLPIQ KFYALDPSFP
     KMNLLTRTQE GKKRQLYMVS KELRNVLLNN SEKMKVINTG IKVWSRNSDG EQFGCAFRLA
     QEGIYTLYPF IHARIVNVCI EDVKILLTQE NPFLSKFSSE TQRKVKDMAM GSIVLKYEPD
     PEKPDDLQCP IVLCGWQGKT SLRAFVPKNE RLHYLRMMGV EVFKAKRKEG ESEGKTEEEV
     QCRPAQTEEG MDVEDKERDA VTKMEAEIDE ESPRSPVESS AMEIENKSED SDQCSKNTNS
     HINQESKDMN TNNVKD
//