Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (cytosine(34)-C(5))-methyltransferase

Gene

NSUN2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytosine(34) in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine(34) in tRNA precursor.

Enzyme regulationi

Inhibited by magnesium ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei215S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei242S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei268S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei321NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytosine(34)-C(5))-methyltransferase (EC:2.1.1.203)
Alternative name(s):
NOL1/NOP2/Sun domain family member 2
Gene namesi
Name:NSUN2
ORF Names:RCJMB04_4l11
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002892251 – 796tRNA (cytosine(34)-C(5))-methyltransferaseAdd BLAST796

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5ZLV4.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000021294.

Structurei

3D structure databases

ProteinModelPortaliQ5ZLV4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni184 – 190S-adenosyl-L-methionine bindingPROSITE-ProRule annotation7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2198. Eukaryota.
COG0144. LUCA.
HOGENOMiHOG000205147.
HOVERGENiHBG106711.
InParanoidiQ5ZLV4.
KOiK15335.
PhylomeDBiQ5ZLV4.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR023270. RCMT_NCL1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02011. RCMTNCL1.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZLV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRRARDRRR QLQPQQRRER SGGGGGGGDD QAGWAGGYPE IVKENELFER
60 70 80 90 100
YYREQRIVPD GEWDAFMAAL REPLPATLRI TGYKSHAREI LHCLKEKYFR
110 120 130 140 150
ELQHLEVDGQ KVEMPQALSW YPEELAWHTN LSRKILRKSP QLERFHQFLV
160 170 180 190 200
SETECGNISR QEAVSMIPPL LLNVNPDHKI LDMCAAPGSK TAQLIEMLHA
210 220 230 240 250
DMNVPFPKGF VIANDVDNKR CYLLVHQAKR LNSPCIMVVN HDASSIPNLQ
260 270 280 290 300
VDVDGRKETL FYDRILCDVP CSGDGTMRKN IDVWKKWTTQ NSLQLHGLQL
310 320 330 340 350
RIATRGVEQL AEGGRMVYST CSLNPIENEA VIASLLEKSQ GALELADVSS
360 370 380 390 400
ELPGLKRMPG ITKWKVMLKD GQWFEEWKDV PSNRQTQIRP TMFPIKEEEK
410 420 430 440 450
LKAMNLERCI RILPHHQNTG GFFVAVLIKK SPMPWNKRQP KVHQKLPGKT
460 470 480 490 500
EDTEVTATNA GDGSEDATEK PTLAEDEEPK KVQELQNSDT EQSKKGVCGP
510 520 530 540 550
PPSKKMKLFG FKEDPFVFLP EDDPLFLPIQ KFYALDPSFP KMNLLTRTQE
560 570 580 590 600
GKKRQLYMVS KELRNVLLNN SEKMKVINTG IKVWSRNSDG EQFGCAFRLA
610 620 630 640 650
QEGIYTLYPF IHARIVNVCI EDVKILLTQE NPFLSKFSSE TQRKVKDMAM
660 670 680 690 700
GSIVLKYEPD PEKPDDLQCP IVLCGWQGKT SLRAFVPKNE RLHYLRMMGV
710 720 730 740 750
EVFKAKRKEG ESEGKTEEEV QCRPAQTEEG MDVEDKERDA VTKMEAEIDE
760 770 780 790
ESPRSPVESS AMEIENKSED SDQCSKNTNS HINQESKDMN TNNVKD
Length:796
Mass (Da):90,845
Last modified:November 23, 2004 - v1
Checksum:iF2C352922B62D9AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719630 mRNA. Translation: CAG31289.1.
RefSeqiNP_001026175.1. NM_001031004.1.
UniGeneiGga.20332.

Genome annotation databases

GeneIDi420938.
KEGGigga:420938.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719630 mRNA. Translation: CAG31289.1.
RefSeqiNP_001026175.1. NM_001031004.1.
UniGeneiGga.20332.

3D structure databases

ProteinModelPortaliQ5ZLV4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000021294.

Proteomic databases

PaxDbiQ5ZLV4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi420938.
KEGGigga:420938.

Organism-specific databases

CTDi54888.

Phylogenomic databases

eggNOGiKOG2198. Eukaryota.
COG0144. LUCA.
HOGENOMiHOG000205147.
HOVERGENiHBG106711.
InParanoidiQ5ZLV4.
KOiK15335.
PhylomeDBiQ5ZLV4.

Miscellaneous databases

PROiQ5ZLV4.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR023270. RCMT_NCL1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02011. RCMTNCL1.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNSUN2_CHICK
AccessioniPrimary (citable) accession number: Q5ZLV4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 23, 2004
Last modified: June 8, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.