Q5ZLV4 (NSUN2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (cytosine(34)-C(5))-methyltransferase
EC=2.1.1.203

Alternative name(s):
NOL1/NOP2/Sun domain family member 2

Gene names

Name:	NSUN2
ORF Names:	RCJMB04_4l11

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	796 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors By similarity.
Catalytic activity	S-adenosyl-L-methionine + cytosine(34) in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine(34) in tRNA precursor.
Subcellular location	Nucleus › nucleolus By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 796	796	tRNA (cytosine(34)-C(5))-methyltransferase		PRO_0000289225
Regions
	Region	184 – 190	7	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	321	1	Nucleophile Potential
	Binding site	215	1	S-adenosyl-L-methionine By similarity
	Binding site	242	1	S-adenosyl-L-methionine By similarity
	Binding site	268	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZLV4 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: F2C352922B62D9AD	FASTA	796	90,845
	10 20 30 40 50 60 MGRRARDRRR QLQPQQRRER SGGGGGGGDD QAGWAGGYPE IVKENELFER YYREQRIVPD 70 80 90 100 110 120 GEWDAFMAAL REPLPATLRI TGYKSHAREI LHCLKEKYFR ELQHLEVDGQ KVEMPQALSW 130 140 150 160 170 180 YPEELAWHTN LSRKILRKSP QLERFHQFLV SETECGNISR QEAVSMIPPL LLNVNPDHKI 190 200 210 220 230 240 LDMCAAPGSK TAQLIEMLHA DMNVPFPKGF VIANDVDNKR CYLLVHQAKR LNSPCIMVVN 250 260 270 280 290 300 HDASSIPNLQ VDVDGRKETL FYDRILCDVP CSGDGTMRKN IDVWKKWTTQ NSLQLHGLQL 310 320 330 340 350 360 RIATRGVEQL AEGGRMVYST CSLNPIENEA VIASLLEKSQ GALELADVSS ELPGLKRMPG 370 380 390 400 410 420 ITKWKVMLKD GQWFEEWKDV PSNRQTQIRP TMFPIKEEEK LKAMNLERCI RILPHHQNTG 430 440 450 460 470 480 GFFVAVLIKK SPMPWNKRQP KVHQKLPGKT EDTEVTATNA GDGSEDATEK PTLAEDEEPK 490 500 510 520 530 540 KVQELQNSDT EQSKKGVCGP PPSKKMKLFG FKEDPFVFLP EDDPLFLPIQ KFYALDPSFP 550 560 570 580 590 600 KMNLLTRTQE GKKRQLYMVS KELRNVLLNN SEKMKVINTG IKVWSRNSDG EQFGCAFRLA 610 620 630 640 650 660 QEGIYTLYPF IHARIVNVCI EDVKILLTQE NPFLSKFSSE TQRKVKDMAM GSIVLKYEPD 670 680 690 700 710 720 PEKPDDLQCP IVLCGWQGKT SLRAFVPKNE RLHYLRMMGV EVFKAKRKEG ESEGKTEEEV 730 740 750 760 770 780 QCRPAQTEEG MDVEDKERDA VTKMEAEIDE ESPRSPVESS AMEIENKSED SDQCSKNTNS 790 HINQESKDMN TNNVKD

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ719630 mRNA. Translation: CAG31289.1.
IPI	IPI00594887.
RefSeq	NP_001026175.1. NM_001031004.1.
UniGene	Gga.20332.
3D structure databases
HSSP	HSSP built from PDB template 2B9E based on UniProtKB Q96P11.
ProteinModelPortal	Q5ZLV4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZLV4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	420938.
KEGG	gga:420938.
Organism-specific databases
CTD	54888.
Phylogenomic databases
eggNOG	veNOG09414.
GeneTree	ENSGT00550000074937.
HOGENOM	HBG315458.
HOVERGEN	HBG106711.
InParanoid	Q5ZLV4.
OrthoDB	EOG4K9BBQ.
PhylomeDB	Q5ZLV4.
Family and domain databases
InterPro	IPR001678. Fmu/NOL1/Nop2p. IPR023267. RCMT. IPR023270. RCMT_NCL1. [Graphical view]
KO	K15335.
Pfam	PF01189. Nol1_Nop2_Fmu. 1 hit. [Graphical view]
PRINTS	PR02008. RCMTFAMILY. PR02011. RCMTNCL1.
PROSITE	PS01153. NOL1_NOP2_SUN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NSUN2_CHICK

Accession

Primary (citable) accession number: Q5ZLV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program