Reviewed, UniProtKB/Swiss-Prot Q5ZLR5 (UCRI_CHICK)

Names and origin

Protein names

Recommended name:
    Cytochrome b-c1 complex subunit Rieske, mitochondrial
    EC=1.10.2.2
Alternative name(s):
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    Rieske iron-sulfur protein
      Short name=RISP
    Complex III subunit 5

Gene names

Name:	UQCRFS1
ORF Names:	RCJMB04_5b19

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis By similarity.
Catalytic activity	QH(2) + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H(+).
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9 and UQCR11/QCR10) By similarity.
Subcellular location	Mitochondrion inner membraneBy similarity.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 76	76	Mitochondrion By similarity
	Chain	77 – 272	196	Cytochrome b-c1 complex subunit Rieske, mitochondrial		PRO_0000342403
Regions
	Domain	185 – 270	86	Rieske
Sites
	Metal binding	215	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	217	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	234	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	237	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	220 ↔ 236		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZLR5-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 3E60AD2419D26C25	FASTA	272	29,387
	10 20 30 40 50 60 MLSVAARSGP FAPYLSAAAH AVPGPLKALA PAALRAEKVV LDLKRPLLCR ESMSGRSARR 70 80 90 100 110 120 DLVAGISLNA PASVRYVHND VTVPDFSAYR REDVMDATTS SQTSSEDRKG FSYLVTATAC 130 140 150 160 170 180 VATAYAAKNV VTQFISSLSA SADVLALSKI EIKLSDIPEG KNVAFKWRGK PLFVRHRTQA 190 200 210 220 230 240 EINQEAEVDV SKLRDPQHDL DRVKKPEWVI LVGVCTHLGC VPIANSGDFG GYYCPCHGSH 250 260 270 YDASGRIRKG PAPYNLEVPT YQFVGDDLVV VG

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ719669 mRNA. Translation: CAG31328.1.
RefSeq	NP_001005843.1.
UniGene	Gga.5791
3D structure databases
SMR	Q5ZLR5. Positions 78-272.
ModBase	Search...
Genome annotation databases
Ensembl	ENSGALG00000004452. Gallus gallus. [Contig view]
GeneID	415752.
KEGG	gga:415752.
Phylogenomic databases
HOVERGEN	Q5ZLR5.
Family and domain databases
InterPro	IPR014349. Rieske. IPR005805. Rieske_C. IPR006317. Rieske_proteo. IPR005806. Rieske_reg. IPR015248. Ubiq_cytc_red_N. IPR004192. UCR_TM_region. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF09165. Ubiq-Cytc-red_N. 1 hit. PF02921. UCR_TM. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_CHICK

Accession

Primary (citable) accession number: Q5ZLR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	November 23, 2004
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)