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Protein

Glycerol-3-phosphate acyltransferase 3

Gene

GPAT3

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May transfer the acyl-group from acyl-coA to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Also transfers the acyl-group from acyl-coA to the sn-2 position of 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid, or LPA), forming 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid, or PA) (By similarity).By similarity

Catalytic activityi

Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.

Pathwayi: triacylglycerol biosynthesis

This protein is involved in the pathway triacylglycerol biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway triacylglycerol biosynthesis and in Glycerolipid metabolism.

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 3 (GPAT3)
  2. Lysocardiolipin acyltransferase 1 (LCLAT1)
  3. Phosphatidate cytidylyltransferase (CDS2), Phosphatidate cytidylyltransferase (CDS1), Phosphatidate cytidylyltransferase, mitochondrial (TAMM41)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

ReactomeiR-GGA-1483166. Synthesis of PA.
R-GGA-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00282.
UPA00557; UER00612.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate acyltransferase 3By similarity (EC:2.3.1.15)
Short name:
GPAT-3
Alternative name(s):
1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9 (EC:2.3.1.51)
Short name:
1-AGP acyltransferase 9
Short name:
1-AGPAT 9
Lysophosphatidic acid acyltransferase theta
Short name:
LPAAT-theta
Gene namesi
Name:GPAT3By similarity
Synonyms:AGPAT9
ORF Names:RCJMB04_5j9
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 4

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei25 – 4521HelicalSequence analysisAdd
BLAST
Transmembranei142 – 16221HelicalSequence analysisAdd
BLAST
Transmembranei164 – 18421HelicalSequence analysisAdd
BLAST
Transmembranei352 – 37221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Glycerol-3-phosphate acyltransferase 3PRO_0000291573Add
BLAST

Proteomic databases

PaxDbiQ5ZLL8.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018276.

Structurei

3D structure databases

ProteinModelPortaliQ5ZLL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi232 – 2376HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2898. Eukaryota.
COG0204. LUCA.
GeneTreeiENSGT00390000000536.
HOGENOMiHOG000265725.
InParanoidiQ5ZLL8.
KOiK13506.
OMAiEVYMKIL.
PhylomeDBiQ5ZLL8.

Family and domain databases

InterProiIPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZLL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELGSLAVW LGAAWLSLVF ALIVLPSALG VSLGISEAYM WVLVKTLEWA
60 70 80 90 100
TIRIEKGVKK PQPQMLKIPA ANGIIERDET PMEKEIAGLH RMEFRFSDIF
110 120 130 140 150
YFCRKGFEAI VEDEVTQRFS SEELVSWNLL TRTNVNFHYV SLRLTVVWVI
160 170 180 190 200
GVIVRYCFLL PLRFTLAAIG ITSMIVGTTV VGQLPNGSLK NYLSEVVHLT
210 220 230 240 250
CSRILVRALS GTIHYHNKEN KPQKGGICVA NHTSPIDAII LTNDGCYAMV
260 270 280 290 300
GQVHGGLMGV IQRATVKACP HVWFERSEIK DRHLVTKRLR EHVADKNKLP
310 320 330 340 350
ILIFPEGTCI NNTSVMMFKK GSFEIGGTIY PVAIKYDPQF GDAFWNSSKY
360 370 380 390 400
NIVSYLLRIM TSWAIVCHVW YMPPMVRKEG EDAVQFANRV RSAIARQGGL
410 420 430 440
TELPWDGGLK RAKVKDSFKE EQQKNYSKML VRNGSQGNLP AGTESD
Length:446
Mass (Da):50,112
Last modified:November 23, 2004 - v1
Checksum:iEE0DCF6D77C3DB6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719716 mRNA. Translation: CAG31375.1.
RefSeqiNP_001026316.1. NM_001031145.1.
UniGeneiGga.16404.

Genome annotation databases

EnsembliENSGALT00000018298; ENSGALP00000018276; ENSGALG00000011221.
GeneIDi422610.
KEGGigga:422610.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719716 mRNA. Translation: CAG31375.1.
RefSeqiNP_001026316.1. NM_001031145.1.
UniGeneiGga.16404.

3D structure databases

ProteinModelPortaliQ5ZLL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000018276.

Proteomic databases

PaxDbiQ5ZLL8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000018298; ENSGALP00000018276; ENSGALG00000011221.
GeneIDi422610.
KEGGigga:422610.

Organism-specific databases

CTDi231510.

Phylogenomic databases

eggNOGiKOG2898. Eukaryota.
COG0204. LUCA.
GeneTreeiENSGT00390000000536.
HOGENOMiHOG000265725.
InParanoidiQ5ZLL8.
KOiK13506.
OMAiEVYMKIL.
PhylomeDBiQ5ZLL8.

Enzyme and pathway databases

UniPathwayiUPA00282.
UPA00557; UER00612.
ReactomeiR-GGA-1483166. Synthesis of PA.
R-GGA-75109. Triglyceride Biosynthesis.

Miscellaneous databases

PROiQ5ZLL8.

Family and domain databases

InterProiIPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiGPAT3_CHICK
AccessioniPrimary (citable) accession number: Q5ZLL8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: November 23, 2004
Last modified: June 8, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Despite its name, the human ortholog of this protein appears to lack measurable glycerol-3-phosphate acyltransferase activity under some conditions (PMID:19318427).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.