Q5ZLL8 (GPAT3_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-3-phosphate acyltransferase 3
Short name=GPAT-3
EC=2.3.1.15

Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 9
Short name=1-AGP acyltransferase 9
Short name=1-AGPAT 9
Lysophosphatidic acid acyltransferase theta
Short name=LPAAT-theta

Gene names

Name:	AGPAT9
Synonyms:	GPAT3
ORF Names:	RCJMB04_5j9

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis By similarity.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis. Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW triglyceride biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Glycerol-3-phosphate acyltransferase 3		PRO_0000291573
Regions
	Transmembrane	25 – 45	21	Helical; Potential
	Transmembrane	142 – 162	21	Helical; Potential
	Transmembrane	164 – 184	21	Helical; Potential
	Transmembrane	352 – 372	21	Helical; Potential
	Motif	232 – 237	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q5ZLL8 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: EE0DCF6D77C3DB6B	FASTA	446	50,112
	10 20 30 40 50 60 MEELGSLAVW LGAAWLSLVF ALIVLPSALG VSLGISEAYM WVLVKTLEWA TIRIEKGVKK 70 80 90 100 110 120 PQPQMLKIPA ANGIIERDET PMEKEIAGLH RMEFRFSDIF YFCRKGFEAI VEDEVTQRFS 130 140 150 160 170 180 SEELVSWNLL TRTNVNFHYV SLRLTVVWVI GVIVRYCFLL PLRFTLAAIG ITSMIVGTTV 190 200 210 220 230 240 VGQLPNGSLK NYLSEVVHLT CSRILVRALS GTIHYHNKEN KPQKGGICVA NHTSPIDAII 250 260 270 280 290 300 LTNDGCYAMV GQVHGGLMGV IQRATVKACP HVWFERSEIK DRHLVTKRLR EHVADKNKLP 310 320 330 340 350 360 ILIFPEGTCI NNTSVMMFKK GSFEIGGTIY PVAIKYDPQF GDAFWNSSKY NIVSYLLRIM 370 380 390 400 410 420 TSWAIVCHVW YMPPMVRKEG EDAVQFANRV RSAIARQGGL TELPWDGGLK RAKVKDSFKE 430 440 EQQKNYSKML VRNGSQGNLP AGTESD

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ719716 mRNA. Translation: CAG31375.1.
IPI	IPI00587916.
RefSeq	NP_001026316.1. NM_001031145.1.
UniGene	Gga.16404.
3D structure databases
ProteinModelPortal	Q5ZLL8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZLL8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	422610.
KEGG	gga:422610.
Organism-specific databases
CTD	84803.
Phylogenomic databases
eggNOG	veNOG05803.
GeneTree	ENSGT00390000000536.
HOGENOM	HBG408354.
InParanoid	Q5ZLL8.
OrthoDB	EOG4Z62NH.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
KO	K13506.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPAT3_CHICK

Accession

Primary (citable) accession number: Q5ZLL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program