Q5ZLL6 (MSMO1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylsterol monooxygenase 1
EC=1.14.13.72

Alternative name(s):
C-4 methylsterol oxidase

Gene names

Name:	MSMO1
Synonyms:	SC4MOL
ORF Names:	RCJMB04_5j19

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)+ + H2O. 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)+ + 2 H2O. 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)+ + H2O.
Cofactor	Iron By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the sterol desaturase family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Iron NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: InterPro sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	C-4 methylsterol oxidase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Methylsterol monooxygenase 1		PRO_0000249852
Regions
	Transmembrane	55 – 75	21	Helical; Potential
	Transmembrane	100 – 120	21	Helical; Potential
	Transmembrane	199 – 219	21	Helical; Potential
	Motif	157 – 161	5	Histidine box-1 By similarity
	Motif	170 – 174	5	Histidine box-2 By similarity
	Motif	249 – 255	7	Histidine box-3 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZLL6 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 54144716232002A0	FASTA	296	35,485
	10 20 30 40 50 60 MAVNDSVNIL NSAYLAVEYI DSFLPDNPLQ QPFKNAWNYM LDNYTKFQIA TWGSLLVHEA 70 80 90 100 110 120 SYFLLCVPGF IFQFIPYMQK YKIQPDKPET WEKQWKCFKT LIFNHFFIQL PLICGTYYFT 130 140 150 160 170 180 EYFNIPYEWE EMPRWYVLLA QCFGCAVIED AWHYFLHRLL HHKRIYKYIH KVHHEFVSPF 190 200 210 220 230 240 GMQAEYAHPL ETLILGAGFF IGIVVFCNHV VLLWAWVICR LMETIDVHSG YDIPLNPLHL 250 260 270 280 290 VPFYAGARFH DFHHMNFIGN YASTFTWWDR IFGTDSQFIA YKEKEKKQQL RMKKTN

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ719718 mRNA. Translation: CAG31377.1.
IPI	IPI00578812.
RefSeq	NP_001006438.1. NM_001006438.1.
UniGene	Gga.21297.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZLL6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	422423.
KEGG	gga:422423.
Organism-specific databases
CTD	66234.
Phylogenomic databases
eggNOG	veNOG09290.
GeneTree	ENSGT00530000063017.
HOGENOM	HBG325679.
HOVERGEN	HBG051504.
InParanoid	Q5ZLL6.
OrthoDB	EOG4CG08K.
PhylomeDB	Q5ZLL6.
Family and domain databases
InterPro	IPR006694. Fatty_acid_hydroxylase. [Graphical view]
KO	K07750.
Pfam	PF04116. FA_hydroxylase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MSMO1_CHICK

Accession

Primary (citable) accession number: Q5ZLL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	November 23, 2004
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program