Reviewed, UniProtKB/Swiss-Prot Q5ZLL6 (ERG25_CHICK)

Names and origin

Protein names

Recommended name:
    C-4 methylsterol oxidase
    EC=1.14.13.72
Alternative name(s):
    Methylsterol monooxygenase

Gene names

Name:	SC4MOL
ORF Names:	RCJMB04_5j19

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)+ + H2O. 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)+ + 2 H2O. 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)+ + H2O.
Cofactor	Iron By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 3/6.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Probable.
Domain	The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similarities	Belongs to the sterol desaturase family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	Iron NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	C-4 methylsterol oxidase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	C-4 methylsterol oxidase		PRO_0000249852
Regions
	Transmembrane	55 – 75	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	199 – 219	21	Potential
	Motif	157 – 161	5	Histidine box-1 By similarity
	Motif	170 – 174	5	Histidine box-2 By similarity
	Motif	249 – 255	7	Histidine box-3 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZLL6-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 54144716232002A0	FASTA	296	35,485
	10 20 30 40 50 60 MAVNDSVNIL NSAYLAVEYI DSFLPDNPLQ QPFKNAWNYM LDNYTKFQIA TWGSLLVHEA 70 80 90 100 110 120 SYFLLCVPGF IFQFIPYMQK YKIQPDKPET WEKQWKCFKT LIFNHFFIQL PLICGTYYFT 130 140 150 160 170 180 EYFNIPYEWE EMPRWYVLLA QCFGCAVIED AWHYFLHRLL HHKRIYKYIH KVHHEFVSPF 190 200 210 220 230 240 GMQAEYAHPL ETLILGAGFF IGIVVFCNHV VLLWAWVICR LMETIDVHSG YDIPLNPLHL 250 260 270 280 290 VPFYAGARFH DFHHMNFIGN YASTFTWWDR IFGTDSQFIA YKEKEKKQQL RMKKTN

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ719718 mRNA. Translation: CAG31377.1.
IPI	IPI00578812.
RefSeq	NP_001006438.1.
UniGene	Gga.21297
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZLL6.
Genome annotation databases
Ensembl	ENSGALT00000015566; ENSGALP00000015550; ENSGALG00000009560; Gallus gallus. [Genome view]
GeneID	422423.
KEGG	gga:422423.
Organism-specific databases
CTD	422423.
Phylogenomic databases
eggNOG	veNOG09290.
HOGENOM	HBG325679.
HOVERGEN	Q5ZLL6.
InParanoid	Q5ZLL6.
PhylomeDB	Q5ZLL6.
Enzyme and pathway databases
BRENDA	1.14.13.72. 4.
Family and domain databases
InterPro	IPR006694. Fatty_acid_hydroxylase. [Graphical view]
Pfam	PF04116. FA_hydroxylase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ERG25_CHICK

Accession

Primary (citable) accession number: Q5ZLL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	November 23, 2004
Last modified:	January 19, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)