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Reviewed, UniProtKB/Swiss-Prot Q5ZLK5 (P4HA2_CHICK)

Last modified February 9, 2010. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Prolyl 4-hydroxylase subunit alpha-2
      Short name=4-PH alpha-2
    EC=1.14.11.2
Alternative name(s):
    Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2
Gene names
Name: P4HA2
ORF Names: RCJMB04_5l17
OrganismGallus gallus (Chicken)
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins By similarity.

Catalytic activity

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactor

Binds 1 Fe2+ ion per subunit By similarity.

Ascorbate By similarity.

Subunit structure

Heterotetramer of two alpha-2 chains and two beta chains (the beta chain is the multi-functional PDI) By similarity.

Subcellular location

Endoplasmic reticulum lumen By similarity.

Sequence similarities

Belongs to the P4HA family.

Contains 1 Fe2OG dioxygenase domain.

Contains 1 TPR repeat.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 534513Prolyl 4-hydroxylase subunit alpha-2
PRO_0000041836

Regions

Repeat207 – 24034TPR
Domain413 – 519107Fe2OG dioxygenase

Sites

Metal binding4311Iron By similarity
Metal binding4331Iron By similarity
Metal binding5001Iron By similarity
Binding site51012-oxoglutarate Potential

Amino acid modifications

Glycosylation1151N-linked (GlcNAc...) Potential
Glycosylation2631N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q5ZLK5-1 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: F3176919EBDEEBB1

FASTA53461,435
        10         20         30         40         50         60 
MKPWLCLVFF TSAFLIWHAE AEFFTSIGQM TDLIYAEKDL VQSLKEYIRA EETKLSQIKS 

        70         80         90        100        110        120 
WAEKMDVLTS KSTSDPEGYL AHPVNAYKLV KRLNTDWLEL ENLVLQDTTN GFITNLTIQR 

       130        140        150        160        170        180 
QFFPTEEDET GAAKALMRLQ DTYKLDPETL SRGNLPGTKY RSSLTVSDCF GMGKTAYNDG 

       190        200        210        220        230        240 
DYYHTVLWME QALKQHDEGE DTTVSKVEIL DYLSYAVFQF GDLHRAMELT RRLISLDSTH 

       250        260        270        280        290        300 
ERAGSNLRYF EKLLEKEREK PSNKTVATTE PVVQSGAYER PLDYLPERDI YEALCRGEGV 

       310        320        330        340        350        360 
KMTPRRQKRL FCRYHDGNRN PHLLIAPFKE EDEWDSPHIV RYYDVMSDEE IEKIKQLAKP 

       370        380        390        400        410        420 
KLARATVRDP KTGVLTVASY RVSKSSWLEE DDDPVVAKVN QRMQQITGLT VKTAELLQVA 

       430        440        450        460        470        480 
NYGMGGQYEP HFDFSRRPFD STLKSEGNRL ATFLNYMSDV EAGGATVFPD FGAAIWPKKG 

       490        500        510        520        530 
TAVFWYNLFR SGEGDYRTRH AACPVLVGCK WVSNKWFHER GNEFLRPCGR TEVD 

« Hide

References

[1]"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis."
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.
Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: CB.
Tissue: Bursa of Fabricius.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ719729 mRNA. Translation: CAG31388.1.
IPIIPI00594034.
RefSeqNP_001006155.1.
UniGeneGga.5799

3D structure databases

SMRQ5ZLK5. Positions 164-256, 336-520.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5ZLK5.

Genome annotation databases

EnsemblENSGALT00000010797; ENSGALP00000010783; ENSGALG00000006677; Gallus gallus. [Genome view]
GeneID416326.
KEGGgga:416326.

Organism-specific databases

CTD416326.

Phylogenomic databases

HOVERGENQ5ZLK5.
InParanoidQ5ZLK5.

Enzyme and pathway databases

BRENDA1.14.11.2. 4.

Family and domain databases

InterProIPR005123. Oxoglutarate/Fe-dep_oxygenase.
IPR006620. Pro_4_hyd_alph.
IPR013547. Pro_4_hyd_alph_N.
IPR011990. TPR-like_helical.
[Graphical view]
Gene3DG3DSA:1.25.40.10. TPR-like_helical. 1 hit.
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
PF08336. P4Ha_N. 1 hit.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
PS50005. TPR. False negative.
PS50293. TPR_REGION. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP4HA2_CHICK
AccessionPrimary (citable) accession number: Q5ZLK5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 23, 2004
Last modified: February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents