Reviewed, UniProtKB/Swiss-Prot Q5ZLK5 (P4HA2_CHICK)

Names and origin

Protein names

Recommended name:
    Prolyl 4-hydroxylase subunit alpha-2
      Short name=4-PH alpha-2
    EC=1.14.11.2
Alternative name(s):
    Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2

Gene names

Name:	P4HA2
ORF Names:	RCJMB04_5l17

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins By similarity.
Catalytic activity	L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-4-hydroxy-L-proline-[procollagen] + succinate + CO2.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity. Ascorbate By similarity.
Subunit structure	Heterotetramer of two alpha-2 chains and two beta chains (the beta chain is the multi-functional PDI) By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Sequence similarities	Belongs to the P4HA family. Contains 1 Fe2OG dioxygenase domain. Contains 1 TPR repeat.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal TPR repeat
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW procollagen-proline 4-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 534	513	Prolyl 4-hydroxylase subunit alpha-2		PRO_0000041836
Regions
	Repeat	207 – 240	34	TPR
	Domain	413 – 519	107	Fe2OG dioxygenase
Sites
	Metal binding	431	1	Iron By similarity
	Metal binding	433	1	Iron By similarity
	Metal binding	500	1	Iron By similarity
	Binding site	510	1	2-oxoglutarate Potential
Amino acid modifications
	Glycosylation	115	1	N-linked (GlcNAc...) Potential
	Glycosylation	263	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5ZLK5-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: F3176919EBDEEBB1	FASTA	534	61,435
	10 20 30 40 50 60 MKPWLCLVFF TSAFLIWHAE AEFFTSIGQM TDLIYAEKDL VQSLKEYIRA EETKLSQIKS 70 80 90 100 110 120 WAEKMDVLTS KSTSDPEGYL AHPVNAYKLV KRLNTDWLEL ENLVLQDTTN GFITNLTIQR 130 140 150 160 170 180 QFFPTEEDET GAAKALMRLQ DTYKLDPETL SRGNLPGTKY RSSLTVSDCF GMGKTAYNDG 190 200 210 220 230 240 DYYHTVLWME QALKQHDEGE DTTVSKVEIL DYLSYAVFQF GDLHRAMELT RRLISLDSTH 250 260 270 280 290 300 ERAGSNLRYF EKLLEKEREK PSNKTVATTE PVVQSGAYER PLDYLPERDI YEALCRGEGV 310 320 330 340 350 360 KMTPRRQKRL FCRYHDGNRN PHLLIAPFKE EDEWDSPHIV RYYDVMSDEE IEKIKQLAKP 370 380 390 400 410 420 KLARATVRDP KTGVLTVASY RVSKSSWLEE DDDPVVAKVN QRMQQITGLT VKTAELLQVA 430 440 450 460 470 480 NYGMGGQYEP HFDFSRRPFD STLKSEGNRL ATFLNYMSDV EAGGATVFPD FGAAIWPKKG 490 500 510 520 530 TAVFWYNLFR SGEGDYRTRH AACPVLVGCK WVSNKWFHER GNEFLRPCGR TEVD

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ719729 mRNA. Translation: CAG31388.1.
IPI	IPI00594034.
RefSeq	NP_001006155.1.
UniGene	Gga.5799
3D structure databases
SMR	Q5ZLK5. Positions 164-256, 336-520.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZLK5.
Genome annotation databases
Ensembl	ENSGALT00000010797; ENSGALP00000010783; ENSGALG00000006677; Gallus gallus. [Genome view]
GeneID	416326.
KEGG	gga:416326.
Organism-specific databases
CTD	416326.
Phylogenomic databases
HOVERGEN	Q5ZLK5.
InParanoid	Q5ZLK5.
Enzyme and pathway databases
BRENDA	1.14.11.2. 4.
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. IPR006620. Pro_4_hyd_alph. IPR013547. Pro_4_hyd_alph_N. IPR011990. TPR-like_helical. [Graphical view]
Gene3D	G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. PF08336. P4Ha_N. 1 hit. [Graphical view]
SMART	SM00702. P4Hc. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. PS50005. TPR. False negative. PS50293. TPR_REGION. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P4HA2_CHICK

Accession

Primary (citable) accession number: Q5ZLK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)