Q5ZL98 (RPC1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase III subunit RPC1
Short name=RNA polymerase III subunit C1
EC=2.7.7.6

Alternative name(s):
DNA-directed RNA polymerase III subunit A

Gene names

Name:	POLR3A
ORF Names:	RCJMB04_7b9

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	1390 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Ligand	Magnesium Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1390	1390	DNA-directed RNA polymerase III subunit RPC1		PRO_0000330753
Regions
	Region	844 – 856	13	Bridging helix By similarity
Sites
	Metal binding	69	1	Zinc 1 By similarity
	Metal binding	72	1	Zinc 1 By similarity
	Metal binding	79	1	Zinc 1 By similarity
	Metal binding	82	1	Zinc 1 By similarity
	Metal binding	109	1	Zinc 2 By similarity
	Metal binding	112	1	Zinc 2 By similarity
	Metal binding	156	1	Zinc 2 By similarity
	Metal binding	499	1	Magnesium; catalytic By similarity
	Metal binding	501	1	Magnesium; catalytic By similarity
	Metal binding	503	1	Magnesium; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZL98 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 82DB8BA3640CEB09	FASTA	1,390	155,704
	10 20 30 40 50 60 MVKEQFRESD VAKKISHICF GMKSPEEMRQ QAHIQVVSKN LYSQDNHHAP LQYGVLDHRM 70 80 90 100 110 120 GTSEKDRPCE TCGKNLADCL GHYGYIDLEL PCFHVGYFKA VIGILQMICK TCCRIMLSVE 130 140 150 160 170 180 EKKQFLDYLK RPGLTYLQKR GLKKKVSEKC RKKNTCPYCG AFNGTVKKCG LLKIIHEKYK 190 200 210 220 230 240 TNKKVVDPIV STFLQSFETA IEHNKEVEPL LGRAQENLNP LVVLNLFKRI PAEDIPLLLM 250 260 270 280 290 300 NPEAGKPSDL ILTRLLVPPL CIRPSVVSDL KSGTNEDDLT MKLTEIIFLN DVIKKHRISG 310 320 330 340 350 360 AKTQMIMEDW DFLQLQCALY INSELSGIPL NMAPKKWTRG FVQRLKGKQG RFRGNLSGKR 370 380 390 400 410 420 VDFSGRTVIS PDPNLRIDEV AVPIHVAKIL TFPEKVNKAN INFMRKLVRN GPDVHPGANF 430 440 450 460 470 480 IQQRHTQMKR FLKYGNREKM AQELKFGDIV ERHLIDGDIV LFNRQPSLHK LSIMAHIARV 490 500 510 520 530 540 KPHRTFRFNE CVCTPYNADF DGDEMNLHLP QTEEAKAEAL VLMGTKANLV TPRNGEPLIA 550 560 570 580 590 600 AIQDFLTGAY LLTLKDTFFD RAKACQIIAS ILVGKDEKIK VRLPPPAILK PVTLWTGKQV 610 620 630 640 650 660 FSSILKPSDD CPVKANLRTK GKQYCGKGED LCYNDSYVTI QNSELMSGSM DKGTLGSGSK 670 680 690 700 710 720 NNIFYILLRD WGQVEAADAM SRLARLAPVY LSNRGFSIGI GDVTPGQGLL KAKYELLHAG 730 740 750 760 770 780 YKKCDEYIEA LNTGKLQQQP GCTAEETLEA LILKELSVIR DHAGSACLRE LDKSNSPLIM 790 800 810 820 830 840 ALCGSKGSFI NISQMIACVG QQAISGSRVP DGFENRSLPH FEKHSKLPAA KGFVANSFYS 850 860 870 880 890 900 GLTPTEFFFH TMAGREGLVD TAVKTAETGY MQRRLVKSLE DLCSQYDLTV RSSTGDIIQF 910 920 930 940 950 960 IYGGDGLDPA AMEGKDEPLE FKRVLDNIRA VYPCRSEPAL SKNELVLTSE SIMKKNEFLC 970 980 990 1000 1010 1020 CRDSFLQEIK KFIKGVSEKI KKTRDKYGIN DNGTTEPRVL YQLDRITPTQ LEKFLETCRD 1030 1040 1050 1060 1070 1080 KYMRAQMEPG SAVGALCAQS IGEPGTQMTL KTFHFAGVAS MNITLGVPRI KEIINASKAI 1090 1100 1110 1120 1130 1140 STPIITAQLD KDDDPDFARL VKGRIEKTLL GEISEYIEEV FLPDDCFILV KLSLERIRLL 1150 1160 1170 1180 1190 1200 RLEVNAETVR YSICISKLRV KPGDVAVHGE AVVCVTPREN SKSSMYYVLQ SLKEELPKVV 1210 1220 1230 1240 1250 1260 VQGIPEVSRA VIHIDEQSGK EKYKLLVEGD NLRAVMATHG VKGTKTSSNN TYEVEKTLGI 1270 1280 1290 1300 1310 1320 EAARTTIINE IQYTMVNHGM SIDRRHVMLL SDLMTYKGEV LGITRFGLAK MKESVLMLAS 1330 1340 1350 1360 1370 1380 FEKTADHLFD AAYFGQKDSV CGVSECIIMG IPMNIGTGLF KLLHKADKES TPPRRPLIFD 1390 NNEFHIPIVT

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ719836 mRNA. Translation: CAG31495.1.
IPI	IPI00580235.
RefSeq	NP_001006500.1. NM_001006500.1.
UniGene	Gga.22202.
3D structure databases
HSSP	HSSP built from PDB template 1R5U based on UniProtKB P04050.
ProteinModelPortal	Q5ZL98.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZL98.
Proteomic databases
PRIDE	Q5ZL98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	423727.
KEGG	gga:423727.
Organism-specific databases
CTD	11128.
Phylogenomic databases
eggNOG	veNOG11979.
GeneTree	ENSGT00550000074864.
HOGENOM	HBG499785.
HOVERGEN	HBG004339.
InParanoid	Q5ZL98.
OrthoDB	EOG4QVCB4.
PhylomeDB	Q5ZL98.
Family and domain databases
InterPro	IPR000722. RNA_pol_asu. IPR015700. RNA_pol_III_lsu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03018.
PANTHER	PTHR19376:SF15. RNA_pol3. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPC1_CHICK

Accession

Primary (citable) accession number: Q5ZL98

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program