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Protein

60 kDa heat shock protein, mitochondrial

Gene

HSPD1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75ATPBy similarity1
Binding sitei440ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei520ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi111 – 115ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone, Hydrolase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
60 kDa heat shock protein, mitochondrial (EC:3.6.4.9)
Alternative name(s):
60 kDa chaperonin
Chaperonin 60
Short name:
CPN60
Heat shock protein 60
Short name:
HSP-60
Short name:
Hsp60
Gene namesi
Name:HSPD1By similarity
Synonyms:HSP60By similarity
ORF Names:RCJMB04_7g5
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 7

Subcellular locationi

  • Mitochondrion matrix By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 26MitochondrionBy similarityAdd BLAST26
ChainiPRO_000022350027 – 57360 kDa heat shock protein, mitochondrialAdd BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei227PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5ZL72.
PRIDEiQ5ZL72.

Expressioni

Gene expression databases

BgeeiENSGALG00000008094.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KCNMA1Q8AYS83EBI-1635874,EBI-1635766

GO - Molecular functioni

Protein-protein interaction databases

BioGridi684495. 5 interactors.
IntActiQ5ZL72. 1 interactor.
STRINGi9031.ENSGALP00000013122.

Structurei

3D structure databases

ProteinModelPortaliQ5ZL72.
SMRiQ5ZL72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chaperonin (HSP60) family.Sequence analysis

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0356. Eukaryota.
COG0459. LUCA.
GeneTreeiENSGT00390000005727.
HOGENOMiHOG000076290.
HOVERGENiHBG001982.
InParanoidiQ5ZL72.
KOiK04077.
OMAiNQHRKPL.
OrthoDBiEOG091G04JM.
PhylomeDBiQ5ZL72.
TreeFamiTF300475.

Family and domain databases

Gene3Di1.10.560.10. 2 hits.
3.50.7.10. 1 hit.
HAMAPiMF_00600. CH60. 1 hit.
InterProiView protein in InterPro
IPR018370. Chaperonin_Cpn60_CS.
IPR001844. Chaprnin_Cpn60.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
PfamiView protein in Pfam
PF00118. Cpn60_TCP1. 1 hit.
PRINTSiPR00298. CHAPERONIN60.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02348. GroEL. 1 hit.
PROSITEiView protein in PROSITE
PS00296. CHAPERONINS_CPN60. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5ZL72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRLPAVLRQ IRPVSRALAP HLTRAYAKDV KFGADARALM LQGVDLLADA
60 70 80 90 100
VAVTMGPKGR TVIIEQSWGS PKVTKDGVTV AKAIDLKDKY KNIGAKLVQD
110 120 130 140 150
VANNTNEEAG DGTTTATVLA RAIAKEGFEK ISKGANPVEI RRGVMLAVDA
160 170 180 190 200
ITAELKKLSK PVTTPEEIAQ VATISANGDQ EIGNIISDAM KKVGRKGVIT
210 220 230 240 250
VKDGKTLNDE LEIIEGMKFD RGYISPYFIN TAKGQKCEFQ DAYVLISEKK
260 270 280 290 300
ISSVQSIVPA LEIANSHRKP LVIIAEDVDG EALSTLVLNR LKVGLQVVAV
310 320 330 340 350
KAPGFGDNRK NQLKDMAIAT GGAVFGEEGL SLNVEDIQPH DFGKVGEVIV
360 370 380 390 400
TKDDTMLLKG KGEKAQIEKR IQEIIEQLEV TTSEYEKEKL NERLAKLSDG
410 420 430 440 450
VAVLKVGGTS DVEVNEKKDR VTDALNATRA AVEEGIVPGG GCALLRCIPA
460 470 480 490 500
LDALKPANED QKIGIEIIKR TLKIPAMTIA KNAGVEGSLI VEKILQSSSE
510 520 530 540 550
VGYDAMLGEF VNMVEKGIID PTKVVRTALM DAAGVASLLS TAEAVVTEVP
560 570
KEEKEPAMGG MGGMGGGMGG GMF
Length:573
Mass (Da):60,973
Last modified:November 23, 2004 - v1
Checksum:i18E4DAAAA0EEEDC6
GO

Mass spectrometryi

Molecular mass is 61122±2 Da from positions 27 - 573. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719862 mRNA. Translation: CAG31521.1.
RefSeqiNP_001012934.1. NM_001012916.2.
UniGeneiGga.39003.

Genome annotation databases

EnsembliENSGALT00000013137; ENSGALP00000013122; ENSGALG00000008094.
GeneIDi424059.
KEGGigga:424059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719862 mRNA. Translation: CAG31521.1.
RefSeqiNP_001012934.1. NM_001012916.2.
UniGeneiGga.39003.

3D structure databases

ProteinModelPortaliQ5ZL72.
SMRiQ5ZL72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi684495. 5 interactors.
IntActiQ5ZL72. 1 interactor.
STRINGi9031.ENSGALP00000013122.

Proteomic databases

PaxDbiQ5ZL72.
PRIDEiQ5ZL72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000013137; ENSGALP00000013122; ENSGALG00000008094.
GeneIDi424059.
KEGGigga:424059.

Organism-specific databases

CTDi3329.

Phylogenomic databases

eggNOGiKOG0356. Eukaryota.
COG0459. LUCA.
GeneTreeiENSGT00390000005727.
HOGENOMiHOG000076290.
HOVERGENiHBG001982.
InParanoidiQ5ZL72.
KOiK04077.
OMAiNQHRKPL.
OrthoDBiEOG091G04JM.
PhylomeDBiQ5ZL72.
TreeFamiTF300475.

Miscellaneous databases

PROiPR:Q5ZL72.

Gene expression databases

BgeeiENSGALG00000008094.

Family and domain databases

Gene3Di1.10.560.10. 2 hits.
3.50.7.10. 1 hit.
HAMAPiMF_00600. CH60. 1 hit.
InterProiView protein in InterPro
IPR018370. Chaperonin_Cpn60_CS.
IPR001844. Chaprnin_Cpn60.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
PfamiView protein in Pfam
PF00118. Cpn60_TCP1. 1 hit.
PRINTSiPR00298. CHAPERONIN60.
SUPFAMiSSF52029. SSF52029. 1 hit.
TIGRFAMsiTIGR02348. GroEL. 1 hit.
PROSITEiView protein in PROSITE
PS00296. CHAPERONINS_CPN60. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCH60_CHICK
AccessioniPrimary (citable) accession number: Q5ZL72
Secondary accession number(s): P84165
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2006
Last sequence update: November 23, 2004
Last modified: May 10, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.