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Protein

Ubiquitin-like modifier-activating enzyme ATG7

Gene

ATG7

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei578 – 5781Glycyl thioester intermediateBy similarity

GO - Molecular functioni

  • Atg12 activating enzyme activity Source: UniProtKB
  • Atg8 activating enzyme activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-GGA-1632852. Macroautophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme ATG7
Alternative name(s):
ATG12-activating enzyme E1 ATG7
Autophagy-related protein 7
Short name:
APG7-like
Gene namesi
Name:ATG7
Synonyms:APG7L
ORF Names:RCJMB04_8l10
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 12

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709Ubiquitin-like modifier-activating enzyme ATG7PRO_0000212809Add
BLAST

Proteomic databases

PaxDbiQ5ZKY2.

Interactioni

Subunit structurei

Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000007877.

Structurei

3D structure databases

ProteinModelPortaliQ5ZKY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.By similarity

Sequence similaritiesi

Belongs to the ATG7 family.Curated

Phylogenomic databases

eggNOGiKOG2337. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00390000017509.
HOGENOMiHOG000162379.
HOVERGENiHBG080877.
InParanoidiQ5ZKY2.
KOiK08337.
OMAiVMAIPMP.
PhylomeDBiQ5ZKY2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006285. Atg7.
IPR032197. Atg7_N.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PANTHERiPTHR10953:SF3. PTHR10953:SF3. 2 hits.
PfamiPF16420. ATG7_N. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01381. E1_like_apg7. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5ZKY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAVSNESQN PVDPGSSKLQ FAPFSSALNV GFWHELTQKK LNEYRLDETP
60 70 80 90 100
KVIKGYYYNG DPSGFPARLT LEYSAFDINA SIPARCCPAF GTLYNTNTFE
110 120 130 140 150
TFKSCDKKSL LEKEANEIWE SIKSGAALEN PMLLNRFLLL TFADLKKYHF
160 170 180 190 200
YYWFCYPALC FPDGIHVIQK PVCLGDRFSL NQIQALQKAY DELCQTEGVT
210 220 230 240 250
AFPYFLIKYH DNSVVVSPLK KWDGFFQDQG GKVTVGVYDP CNLSHYPGWP
260 270 280 290 300
LRNFLILASH KWGNILQSIE VLCFRDRTMQ GVRDITHSII FEIKLPQGAF
310 320 330 340 350
GPDCPKAVGW EKNQKGGMGP RVVNLSECMD PKRLAESSVD LNLKLMCWRL
360 370 380 390 400
VPTLDLEKIV SAKCLLLGAG TLGCSVARTL MGWGVRKITF VDNARISYSN
410 420 430 440 450
PVRQPLYEFE DCLSGGKPKA LAAAERLQKI FPGVNSEGYN MSIPMPGHPV
460 470 480 490 500
NFSEVTMAQA RKDVATLEEL IDAHDVVFLL MDTRESRWLP AVIAASKRKL
510 520 530 540 550
VINAALGFDT FVVMRHGLKK PKQQETGNAC FSTAPGPSDL LGSSLFSNIP
560 570 580 590 600
GYKLGCYFCN DVVAPGDSTR DRTLDQQCTV SRPGLAMIAG ALAVELMVSV
610 620 630 640 650
LQHPEGGYAV ASSSDDRMNE PPTSLGLVPH QIRGFLSRFD NVLPVSLAFD
660 670 680 690 700
KCTACSPKVL DQYEREGFNF LAKVFNSSHS FLEDLTGLTL LHQETQAAEI

WDMSDDETV
Length:709
Mass (Da):78,800
Last modified:November 23, 2004 - v1
Checksum:i3B0060E12C49E15C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719952 mRNA. Translation: CAG31611.1.
RefSeqiNP_001025763.1. NM_001030592.1.
XP_015148538.1. XM_015293052.1.
XP_015148539.1. XM_015293053.1.
XP_015148540.1. XM_015293054.1.
UniGeneiGga.22358.

Genome annotation databases

EnsembliENSGALT00000036275; ENSGALP00000035497; ENSGALG00000004932.
GeneIDi415961.
KEGGigga:415961.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ719952 mRNA. Translation: CAG31611.1.
RefSeqiNP_001025763.1. NM_001030592.1.
XP_015148538.1. XM_015293052.1.
XP_015148539.1. XM_015293053.1.
XP_015148540.1. XM_015293054.1.
UniGeneiGga.22358.

3D structure databases

ProteinModelPortaliQ5ZKY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000007877.

Proteomic databases

PaxDbiQ5ZKY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000036275; ENSGALP00000035497; ENSGALG00000004932.
GeneIDi415961.
KEGGigga:415961.

Organism-specific databases

CTDi10533.

Phylogenomic databases

eggNOGiKOG2337. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00390000017509.
HOGENOMiHOG000162379.
HOVERGENiHBG080877.
InParanoidiQ5ZKY2.
KOiK08337.
OMAiVMAIPMP.
PhylomeDBiQ5ZKY2.

Enzyme and pathway databases

ReactomeiR-GGA-1632852. Macroautophagy.

Miscellaneous databases

NextBioi20819485.
PROiQ5ZKY2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006285. Atg7.
IPR032197. Atg7_N.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PANTHERiPTHR10953:SF3. PTHR10953:SF3. 2 hits.
PfamiPF16420. ATG7_N. 1 hit.
PF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01381. E1_like_apg7. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiATG7_CHICK
AccessioniPrimary (citable) accession number: Q5ZKY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: November 23, 2004
Last modified: May 11, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.