Q5ZKN1 (CDK9_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 9
EC=2.7.11.22
EC=2.7.11.23

Alternative name(s):
Cell division protein kinase 9

Gene names

Name:	CDK9
ORF Names:	RCJMB04_9p8

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.
Subunit structure	Associates with cyclin-T to form P-TEFb. Also associates with cyclin-K By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC cellular response to cytokine stimulus Inferred from electronic annotation. Source: Compara negative regulation of cell cycle arrest Inferred from electronic annotation. Source: Compara regulation of DNA repair Inferred from electronic annotation. Source: Compara regulation of histone modification Inferred from electronic annotation. Source: Compara regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW replication fork arrest Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	PML body Inferred from electronic annotation. Source: Compara
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC snRNA binding Inferred from electronic annotation. Source: Compara transcription regulatory region DNA binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Cyclin-dependent kinase 9		PRO_0000085803
Regions
	Domain	19 – 315	297	Protein kinase
	Nucleotide binding	25 – 33	9	ATP By similarity
Sites
	Active site	149	1	Proton acceptor By similarity
	Binding site	48	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZKN1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: C16F2AADEB04C4CB	FASTA	372	42,803
	10 20 30 40 50 60 MAKQYDMVEC PFCDEVSKYE KLAKIGQGTF GEVFKAKHRQ TGKKVALKKV LMENEKEGFP 70 80 90 100 110 120 ITALREIKIL QLLKHENVVN LIEICRTKAS PYNRCKGSIY LVFDFCEHDL AGLLSNTHVK 130 140 150 160 170 180 FTLSEIKKVM QMLLNGLYYI HRNKILHRDM KAANVLITRD GVLKLADFGL ARAFSLAKNS 190 200 210 220 230 240 QPNRYTNRVV TLWYRPPELL LGERDYGPPI DLWGGGCIMA EMWTRSPIMQ GNTEQHQLTL 250 260 270 280 290 300 ISQLCGSITP EVWPNVDKYE LYQKLELPKG QKRKVKDRLK AYVKDPYALD LIDKLLVLDP 310 320 330 340 350 360 AQRIDSDDAL NHDFFWSDPM PSDLKNMLST HNQSMFEYLA PPRRRGGHMP QQPANQGRNP 370 AATNQTEFDR VF

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ720053 mRNA. Translation: CAG31712.1.
IPI	IPI00593778.
RefSeq	NP_001006201.1. NM_001006201.2.
UniGene	Gga.2483. Gga.51307.
3D structure databases
ProteinModelPortal	Q5ZKN1.
SMR	Q5ZKN1. Positions 8-325.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000008078.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000008092; ENSGALP00000008078; ENSGALG00000005045.
GeneID	417226.
KEGG	gga:417226.
Organism-specific databases
CTD	1025.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00690000102025.
HOGENOM	HOG000233024.
HOVERGEN	HBG014652.
InParanoid	Q5ZKN1.
KO	K02211.
OMA	LMDNEKE.
OrthoDB	EOG4SF963.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20820567.

Entry information

Entry name

CDK9_CHICK

Accession

Primary (citable) accession number: Q5ZKN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program