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Protein

N-acetylneuraminate lyase

Gene

NPL

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway (By similarity).By similarity

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei142 – 1421Involved in proton transfer during cleavageBy similarity
Active sitei172 – 1721Schiff-base intermediate with substrateBy similarity

GO - Molecular functioni

  1. N-acetylneuraminate lyase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Short name:
NALase
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialate-pyruvate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:NPL
ORF Names:RCJMB04_11j23
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308N-acetylneuraminate lyasePRO_0000273357Add
BLAST

Proteomic databases

PaxDbiQ5ZKD4.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038643.

Structurei

3D structure databases

ProteinModelPortaliQ5ZKD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 512Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.Curated

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ5ZKD4.
KOiK01639.
PhylomeDBiQ5ZKD4.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.

Sequencei

Sequence statusi: Complete.

Q5ZKD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPRKKLEGL VAATVTPMTP DGRINLSVIH QYVDYLVSEQ NVKNIFVNGT
60 70 80 90 100
TGEGLSLSIQ ERKQLAEEWM CQGKGKLDHV IIHVGALSLL ESQELARHAA
110 120 130 140 150
AIGASGIAVI APSFFKPTNK DELLGFLQKV ASEAPTVPFY YYHIPAMTGV
160 170 180 190 200
KIRVEELLDG IREQIPTFQG VKFSDTDLLD LAQCINKKER EQFVFLYGVD
210 220 230 240 250
EQLLSALAIG ANGAVGSTYN YLGRKTNLML QAFAKPDLAL ARKYQFLTGE
260 270 280 290 300
FLSFVIKLGF GVAQTKAVMT SISGIPMGPP RLPLVGASEE FIAKAKAKLE

SIVWPDGD
Length:308
Mass (Da):33,646
Last modified:November 23, 2004 - v1
Checksum:iFBA9375250252359
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720150 mRNA. Translation: CAG31809.1.
RefSeqiNP_001026731.1. NM_001031560.2.
UniGeneiGga.22620.

Genome annotation databases

GeneIDi429074.
KEGGigga:429074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720150 mRNA. Translation: CAG31809.1.
RefSeqiNP_001026731.1. NM_001031560.2.
UniGeneiGga.22620.

3D structure databases

ProteinModelPortaliQ5ZKD4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038643.

Proteomic databases

PaxDbiQ5ZKD4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi429074.
KEGGigga:429074.

Organism-specific databases

CTDi80896.

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
InParanoidiQ5ZKD4.
KOiK01639.
PhylomeDBiQ5ZKD4.

Enzyme and pathway databases

UniPathwayiUPA00629.

Miscellaneous databases

NextBioi20829905.
PROiQ5ZKD4.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiNPL_CHICK
AccessioniPrimary (citable) accession number: Q5ZKD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: November 23, 2004
Last modified: February 4, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.