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Q5ZKD4

- NPL_CHICK

UniProt

Q5ZKD4 - NPL_CHICK

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Protein

N-acetylneuraminate lyase

Gene
NPL, RCJMB04_11j23
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid (Neu5Gc) degradation pathway By similarity.

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei142 – 1421Involved in proton transfer during cleavage By similarity
Active sitei172 – 1721Schiff-base intermediate with substrate By similarity

GO - Molecular functioni

  1. N-acetylneuraminate lyase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00629.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Short name:
NALase
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialate-pyruvate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:NPL
ORF Names:RCJMB04_11j23
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 308308N-acetylneuraminate lyasePRO_0000273357Add
BLAST

Proteomic databases

PaxDbiQ5ZKD4.

Interactioni

Subunit structurei

Homotetramer By similarity.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038643.

Structurei

3D structure databases

ProteinModelPortaliQ5ZKD4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni50 – 512Substrate binding By similarity

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000218206.
HOVERGENiHBG082055.
KOiK01639.
PhylomeDBiQ5ZKD4.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.

Sequencei

Sequence statusi: Complete.

Q5ZKD4-1 [UniParc]FASTAAdd to Basket

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MTPRKKLEGL VAATVTPMTP DGRINLSVIH QYVDYLVSEQ NVKNIFVNGT    50
TGEGLSLSIQ ERKQLAEEWM CQGKGKLDHV IIHVGALSLL ESQELARHAA 100
AIGASGIAVI APSFFKPTNK DELLGFLQKV ASEAPTVPFY YYHIPAMTGV 150
KIRVEELLDG IREQIPTFQG VKFSDTDLLD LAQCINKKER EQFVFLYGVD 200
EQLLSALAIG ANGAVGSTYN YLGRKTNLML QAFAKPDLAL ARKYQFLTGE 250
FLSFVIKLGF GVAQTKAVMT SISGIPMGPP RLPLVGASEE FIAKAKAKLE 300
SIVWPDGD 308
Length:308
Mass (Da):33,646
Last modified:November 23, 2004 - v1
Checksum:iFBA9375250252359
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ720150 mRNA. Translation: CAG31809.1.
RefSeqiNP_001026731.1. NM_001031560.2.
UniGeneiGga.22620.

Genome annotation databases

GeneIDi429074.
KEGGigga:429074.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ720150 mRNA. Translation: CAG31809.1 .
RefSeqi NP_001026731.1. NM_001031560.2.
UniGenei Gga.22620.

3D structure databases

ProteinModelPortali Q5ZKD4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000038643.

Proteomic databases

PaxDbi Q5ZKD4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 429074.
KEGGi gga:429074.

Organism-specific databases

CTDi 80896.

Phylogenomic databases

eggNOGi COG0329.
HOGENOMi HOG000218206.
HOVERGENi HBG082055.
KOi K01639.
PhylomeDBi Q5ZKD4.

Enzyme and pathway databases

UniPathwayi UPA00629 .

Miscellaneous databases

NextBioi 20829905.
PROi Q5ZKD4.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR002220. DapA-like.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiNPL_CHICK
AccessioniPrimary (citable) accession number: Q5ZKD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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