Reviewed, UniProtKB/Swiss-Prot Q5ZKA5 (MTDC_CHICK)

Names and origin

Protein names

Recommended name:
    Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Including the following 2 domains:
    1- Recommended name:
            NAD-dependent methylenetetrahydrofolate dehydrogenase
              EC=1.5.1.15
    2- Recommended name:
            Methenyltetrahydrofolate cyclohydrolase
              EC=3.5.4.9

Gene names

Name:	MTHFD2
ORF Names:	RCJMB04_12b8

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	5,10-methylenetetrahydrofolate + NAD+ = 5,10-methenyltetrahydrofolate + NADH. 5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.
Cofactor	Magnesium By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Magnesium NAD
Molecular function	Hydrolase Oxidoreductase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW methenyltetrahydrofolate cyclohydrolase activity Inferred from electronic annotation. Source: EC methylenetetrahydrofolate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 30	30	Mitochondrion Potential
	Chain	31 – 337	307	Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial		PRO_0000042885

Sequences

	Sequence		Length	Mass (Da)
	Q5ZKA5-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: CA3B321FC58F32CC	FASTA	337	36,411
	10 20 30 40 50 60 MATALCPLRA LGQTAFRPRT RRLHLSAPRA DAVVISGRKL ARQIRQEARH EVEQWVAAGN 70 80 90 100 110 120 KRPHLSVVLV GENPASHSYV LNKTKAAADV GISSETILKP ASITEEELLD LISKLNNDAN 130 140 150 160 170 180 VDGLLVQLPL PEHIDERKIC NAVTPDKDVD GFHVINVGRM CLDQYSMLPA TPWGVWEIIK 190 200 210 220 230 240 RTGIPTLGKN VVVAGRSKNV GMPIAMLLHT DGRHERPGGD ATVTISHRYT PKEQLKQHTI 250 260 270 280 290 300 RADIVVAAAG IPNLITADMI KEGAAVIDVG ITRVQDPITA KSRLVGDVDF EGVKKKASYI 310 320 330 TPVPGGVGPM TVAMLMKNTI IAAKKLLKPK ALEALTA

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ720179 mRNA. Translation: CAG31838.1.
IPI	IPI00588640.
RefSeq	NP_001026531.1.
UniGene	Gga.2672
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSGALG00000013480. Gallus gallus. [Contig view]
GeneID	426126.
KEGG	gga:426126.
Phylogenomic databases
HOVERGEN	Q5ZKA5.
Enzyme and pathway databases
BRENDA	1.5.1.15. 4. 3.5.4.9. 4.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR000672. THF_DH/CycHdrlase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00763. THF_DHG_CYH. 1 hit. PF02882. THF_DHG_CYH_C. 1 hit. [Graphical view]
PRINTS	PR00085. THFDHDRGNASE.
ProDom	PD002300. THFDhg/Cyc_hydro. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00766. THF_DHG_CYH_1. 1 hit. PS00767. THF_DHG_CYH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTDC_CHICK

Accession

Primary (citable) accession number: Q5ZKA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 23, 2004
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)