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Q5ZKA2 (SYIM_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase, mitochondrial

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:IARS2
ORF Names:RCJMB04_12b19
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length1000 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Subcellular location

Mitochondrion matrix By similarity HAMAP-Rule MF_02002.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2727Mitochondrion Potential
Chain28 – 1000973Isoleucine--tRNA ligase, mitochondrial HAMAP-Rule MF_02002
PRO_0000233338

Regions

Motif102 – 11211"HIGH" region By similarity
Motif649 – 6535"KMSKS" region By similarity

Sites

Binding site6521ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5ZKA2 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 4EC0778C2FFEB7ED

FASTA1,000112,007
        10         20         30         40         50         60 
MLGAWRAAPR LRLRARFGVA SVWARSAASE ANSRRPAEES RQDSQYRDTV LLPHSRFAAQ 

        70         80         90        100        110        120 
LPGRLQPDTE LETQQKSGFL ELYSWQRQRK AKQEFCLHDG PPYANGDPHV GHALNKILKD 

       130        140        150        160        170        180 
ITNRFHMMRG YKVHYVPGWD CHGLPIELKA LSEVKGAENL SPVEIRQKAK EFAERAIEKQ 

       190        200        210        220        230        240 
KAAFIRWGIM ADWANCYRTF DPKYEANQLR VFHKMYDKGF IYQDYKPVFW SPSAKTALAE 

       250        260        270        280        290        300 
AELEYNEQHV SRSVYMKFPL LKSPPKLASV IDGSSPASVL VWTTQPWTVP ANQAVCYMPD 

       310        320        330        340        350        360 
AEYSVVKCAT TGEHLILAAD RVESTAAVLD TQFEVISTCK GVDLADGSCA HPTIAGRVSP 

       370        380        390        400        410        420 
LLPANHVTMT KGTGLVHTAP AHGMEDYNVA SHHQLPTECL VDESGHFTEA AGPELKNKNV 

       430        440        450        460        470        480 
LEEGNEAVIK MLQAAGSLLK EEKYVHSYPY DWRTKKPMII RASKQWFVNT ANVKATAQEA 

       490        500        510        520        530        540 
LKKVKIIPTS AVNRMLEMLD RRTFWCISRQ RCWGVPIPVF YQKDTGESLI NSETIADVIK 

       550        560        570        580        590        600 
IVEQQGTDAW WTLPIEQLLS KEAVAKAGGH NVLDYVKGQD VLDIWFDSGT SWAHVLEGAE 

       610        620        630        640        650        660 
QRADVYLEGK DQLGGWFQSS LLTSVATRKK APYKTLVVHG FTLGEKGEKM SKSIGNVVDP 

       670        680        690        700        710        720 
DVVINGGDDH TKDPPYGADV LRWWVAESNV FTEVLIGPVV LNAARDDINK LRNTLRFMLG 

       730        740        750        760        770        780 
NMAGFNPETD SIPPSEMYIV DQYMLHLLQD YGSKVTEAYK EYDYSKVVRL LQAFCSRNLS 

       790        800        810        820        830        840 
NFYFSIIKDR LYCEEEKDPK RRSCQTVLAE ALDVVVRSFA PILPHLAEEV FQHLPYKKDS 

       850        860        870        880        890        900 
EGVFRTGWIN ASSGWKKPGI EEAIEGACAM RDSFLGSISG KNALEYEVII VIEPGLLFEL 

       910        920        930        940        950        960 
MEALQAEESS RVSQLNEIMM ASQTTLLSEI PKETPADANI VKGNFLINLE GGDIREESSY 

       970        980        990       1000 
QVIALPTTKA KCPRCRRYTS DSSSTPCPRC LKVIAGKGST 

« Hide

References

[1]"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis."
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.
Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: CB.
Tissue: Bursa of Fabricius.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ720182 mRNA. Translation: CAG31841.1.
RefSeqNP_001006397.1. NM_001006397.1.
UniGeneGga.7186.

3D structure databases

ProteinModelPortalQ5ZKA2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000037780.

Proteomic databases

PaxDbQ5ZKA2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID421346.
KEGGgga:421346.

Organism-specific databases

CTD55699.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
HOVERGENHBG059862.
InParanoidQ5ZKA2.
KOK01870.
PhylomeDBQ5ZKA2.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20824124.
PROQ5ZKA2.

Entry information

Entry nameSYIM_CHICK
AccessionPrimary (citable) accession number: Q5ZKA2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries