Reviewed, UniProtKB/Swiss-Prot Q5ZKA2 (SYIM_CHICK)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase, mitochondrial
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	IARS2
ORF Names:	RCJMB04_12b19

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	1000 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion Potential
	Chain	28 – 1000	973	Isoleucyl-tRNA synthetase, mitochondrial		PRO_0000233338
Regions
	Motif	102 – 112	11	"HIGH" region By similarity
	Motif	649 – 653	5	"KMSKS" region By similarity
Sites
	Binding site	652	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZKA2-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 4EC0778C2FFEB7ED	FASTA	1,000	112,007
	10 20 30 40 50 60 MLGAWRAAPR LRLRARFGVA SVWARSAASE ANSRRPAEES RQDSQYRDTV LLPHSRFAAQ 70 80 90 100 110 120 LPGRLQPDTE LETQQKSGFL ELYSWQRQRK AKQEFCLHDG PPYANGDPHV GHALNKILKD 130 140 150 160 170 180 ITNRFHMMRG YKVHYVPGWD CHGLPIELKA LSEVKGAENL SPVEIRQKAK EFAERAIEKQ 190 200 210 220 230 240 KAAFIRWGIM ADWANCYRTF DPKYEANQLR VFHKMYDKGF IYQDYKPVFW SPSAKTALAE 250 260 270 280 290 300 AELEYNEQHV SRSVYMKFPL LKSPPKLASV IDGSSPASVL VWTTQPWTVP ANQAVCYMPD 310 320 330 340 350 360 AEYSVVKCAT TGEHLILAAD RVESTAAVLD TQFEVISTCK GVDLADGSCA HPTIAGRVSP 370 380 390 400 410 420 LLPANHVTMT KGTGLVHTAP AHGMEDYNVA SHHQLPTECL VDESGHFTEA AGPELKNKNV 430 440 450 460 470 480 LEEGNEAVIK MLQAAGSLLK EEKYVHSYPY DWRTKKPMII RASKQWFVNT ANVKATAQEA 490 500 510 520 530 540 LKKVKIIPTS AVNRMLEMLD RRTFWCISRQ RCWGVPIPVF YQKDTGESLI NSETIADVIK 550 560 570 580 590 600 IVEQQGTDAW WTLPIEQLLS KEAVAKAGGH NVLDYVKGQD VLDIWFDSGT SWAHVLEGAE 610 620 630 640 650 660 QRADVYLEGK DQLGGWFQSS LLTSVATRKK APYKTLVVHG FTLGEKGEKM SKSIGNVVDP 670 680 690 700 710 720 DVVINGGDDH TKDPPYGADV LRWWVAESNV FTEVLIGPVV LNAARDDINK LRNTLRFMLG 730 740 750 760 770 780 NMAGFNPETD SIPPSEMYIV DQYMLHLLQD YGSKVTEAYK EYDYSKVVRL LQAFCSRNLS 790 800 810 820 830 840 NFYFSIIKDR LYCEEEKDPK RRSCQTVLAE ALDVVVRSFA PILPHLAEEV FQHLPYKKDS 850 860 870 880 890 900 EGVFRTGWIN ASSGWKKPGI EEAIEGACAM RDSFLGSISG KNALEYEVII VIEPGLLFEL 910 920 930 940 950 960 MEALQAEESS RVSQLNEIMM ASQTTLLSEI PKETPADANI VKGNFLINLE GGDIREESSY 970 980 990 1000 QVIALPTTKA KCPRCRRYTS DSSSTPCPRC LKVIAGKGST

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ720182 mRNA. Translation: CAG31841.1.
IPI	IPI00578980.
RefSeq	NP_001006397.1.
UniGene	Gga.7186
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZKA2.
Genome annotation databases
Ensembl	ENSGALT00000015581; ENSGALP00000015565; ENSGALG00000009566; Gallus gallus. [Genome view] ENSGALT00000038573; ENSGALP00000037780; ENSGALG00000009566; Gallus gallus. [Genome view]
GeneID	421346.
KEGG	gga:421346.
Organism-specific databases
CTD	421346.
Phylogenomic databases
HOVERGEN	Q5ZKA2.
Enzyme and pathway databases
BRENDA	6.1.1.5. 4.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYIM_CHICK

Accession

Primary (citable) accession number: Q5ZKA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	November 23, 2004
Last modified:	September 1, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)