Q5ZK92 (SPAST_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spastin
EC=3.6.4.3

Gene names

Name:	SPAST
Synonyms:	SPG4
ORF Names:	RCJMB04_12e12

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Microtubule severing may promote reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Required for membrane traffic from the endoplasmic reticulum (ER) to the Golgi and for completion of the abscission stage of cytokinesis. Also plays a role in axon growth and the formation of axonal branches By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Cytoplasm › perinuclear region By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Membrane Microtubule Nucleus
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW microtubule bundle formation Inferred from sequence or structural similarity. Source: UniProtKB microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB nervous system development Inferred from electronic annotation. Source: UniProtKB-KW protein hexamerization Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alpha-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB beta-tubulin binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5ZK92-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5ZK92-2)

The sequence of this isoform differs from the canonical sequence as follows:
     193-224: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Spastin		PRO_0000367136
Regions
	Transmembrane	62 – 82	21	Helical; Potential
	Domain	116 – 192	77	MIT
	Nucleotide binding	379 – 386	8	ATP Potential
Natural variations
	Alternative sequence	193 – 224	32	Missing in isoform 2.		VSP_036648
Experimental info
	Sequence conflict	45	1	A → T in ACF60960. Ref.1
	Sequence conflict	549	1	L → S in ACF60960. Ref.1
	Sequence conflict	549	1	L → S in ACF60961. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: CC162F0D5CFC1C81	FASTA	613	66,266
	10 20 30 40 50 60 MNSPGGRGKK KGSAGSSSAP PAAGASPSAP SGPAPPAPPA GAAAAAAASP HKRNLYYFSY 70 80 90 100 110 120 PLFAAFALLR FVAFQLGLLV AWLCERLSRG ALMAAKSSRA GDAPEPGGAA ERVRACHKRA 130 140 150 160 170 180 FECISMALRI DEDERAGQKE QAVEWYKKGI EELERGIAVL VVGQGDQCER ARRLQSKMMT 190 200 210 220 230 240 NLAMAKDRLQ LLEKLQADLQ ISKPQMEVYN DSTNLACRNG HLQSESGAVP KKKDPLTHTS 250 260 270 280 290 300 NSLPRSKTVA KTGSTGLSGH HRTPSYSGIS TASVSRPAAN PATSTHKAAP KNSRTNKPST 310 320 330 340 350 360 PTPAARKKKD TKVFRNVDSN LANLILNEIV DSGPAVKFDD IAGQELAKQA LQEIVILPSL 370 380 390 400 410 420 RPELFTGLRA PARGLLLFGP PGNGKTMLAK AVAAESNATF FNISAASLTS KYVGEGEKLV 430 440 450 460 470 480 RALFAVAREL QPSIIFIDEV DSLLCERREG EHDASRRLKT EFLIEFDGVQ SSGEDRILVM 490 500 510 520 530 540 GATNRPQELD DAVLRRFTKR VYVSLPNEET RLILLKNLLS KQGSPLTQKE LAQLARMTDG 550 560 570 580 590 600 YSGSDLTALV KDAALGPIRE LKPEQVKNMS ASEMRNIKLS DFTESLKKIK RSLSPQTLEA 610 YIRWNKDFGD TTV
	Isoform 2 [UniParc]. Checksum: 3C49D847834F1F0D	FASTA	581	62,652
	10 20 30 40 50 60 MNSPGGRGKK KGSAGSSSAP PAAGASPSAP SGPAPPAPPA GAAAAAAASP HKRNLYYFSY 70 80 90 100 110 120 PLFAAFALLR FVAFQLGLLV AWLCERLSRG ALMAAKSSRA GDAPEPGGAA ERVRACHKRA 130 140 150 160 170 180 FECISMALRI DEDERAGQKE QAVEWYKKGI EELERGIAVL VVGQGDQCER ARRLQSKMMT 190 200 210 220 230 240 NLAMAKDRLQ LLESGAVPKK KDPLTHTSNS LPRSKTVAKT GSTGLSGHHR TPSYSGISTA 250 260 270 280 290 300 SVSRPAANPA TSTHKAAPKN SRTNKPSTPT PAARKKKDTK VFRNVDSNLA NLILNEIVDS 310 320 330 340 350 360 GPAVKFDDIA GQELAKQALQ EIVILPSLRP ELFTGLRAPA RGLLLFGPPG NGKTMLAKAV 370 380 390 400 410 420 AAESNATFFN ISAASLTSKY VGEGEKLVRA LFAVARELQP SIIFIDEVDS LLCERREGEH 430 440 450 460 470 480 DASRRLKTEF LIEFDGVQSS GEDRILVMGA TNRPQELDDA VLRRFTKRVY VSLPNEETRL 490 500 510 520 530 540 ILLKNLLSKQ GSPLTQKELA QLARMTDGYS GSDLTALVKD AALGPIRELK PEQVKNMSAS 550 560 570 580 EMRNIKLSDF TESLKKIKRS LSPQTLEAYI RWNKDFGDTT V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Redundant microtubule severing proteins in neurons." Karabay A., Akbalik G. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [MRNA] OF 93-613 (ISOFORM 2).
[2]	"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EU849599 mRNA. Translation: ACF60960.1. EU849600 mRNA. Translation: ACF60961.1. AJ720192 mRNA. Translation: CAG31851.1.
IPI	IPI00599182. IPI00923811.
RefSeq	NP_001026232.1. NM_001031061.1.
UniGene	Gga.21964.
3D structure databases
HSSP	HSSP built from PDB template 1XWI based on UniProtKB O75351.
ProteinModelPortal	Q5ZK92.
SMR	Q5ZK92. Positions 321-605.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZK92.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	421481.
KEGG	gga:421481.
Organism-specific databases
CTD	6683.
Phylogenomic databases
eggNOG	veNOG06140.
GeneTree	ENSGT00570000078874.
HOGENOM	HBG724153.
HOVERGEN	HBG108502.
InParanoid	Q5ZK92.
OrthoDB	EOG4NZTTF.
PhylomeDB	Q5ZK92.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. IPR017179. Spastin. [Graphical view]
KO	K13254.
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
PIRSF	PIRSF037338. Spastin. 1 hit.
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_CHICK

Accession

Primary (citable) accession number: Q5ZK92
Secondary accession number(s): B5AH49, B5AH50

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program