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Q5ZJU3

- ASNS_CHICK

UniProt

Q5ZJU3 - ASNS_CHICK

Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

ASNS

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei97 – 971GlutamineBy similarity
    Binding sitei256 – 2561ATP; via carbonyl oxygenBy similarity
    Binding sitei288 – 2881ATP; via amide nitrogen and carbonyl oxygenBy similarity
    Sitei365 – 3651Important for beta-aspartyl-AMP intermediate formationBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi363 – 3642ATPBy similarity

    GO - Molecular functioni

    1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Asparagine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00134; UER00195.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
    Alternative name(s):
    Glutamine-dependent asparagine synthetase
    Gene namesi
    Name:ASNS
    ORF Names:RCJMB04_15l3
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 561560Asparagine synthetase [glutamine-hydrolyzing]PRO_0000056915Add
    BLAST

    Proteomic databases

    PaxDbiQ5ZJU3.
    PRIDEiQ5ZJU3.

    Interactioni

    Protein-protein interaction databases

    BioGridi681517. 1 interaction.
    STRINGi9031.ENSGALP00000015846.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5ZJU3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 191190Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini213 – 536324Asparagine synthetaseAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni49 – 535Glutamine bindingBy similarity
    Regioni75 – 773Glutamine bindingBy similarity

    Sequence similaritiesi

    Contains 1 asparagine synthetase domain.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0367.
    HOGENOMiHOG000027493.
    HOVERGENiHBG003103.
    InParanoidiQ5ZJU3.
    KOiK01953.
    PhylomeDBiQ5ZJU3.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PfamiPF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMiSSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5ZJU3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIWALFGS DECLSVQCLS AMKIAHRGPD AFRFENVNGF TNCCFGFHRL    50
    AVVDQLYGMQ PIRVKKFPYL WLCYNGEIYN FKQLQEQFGF EYQTLVDGEV 100
    ILHLYNRGGI EQTASMLDGV FAFILLDTAN RKVFLARDTY GVRPLFKVLT 150
    DDGFLGVCSE AKGLINLKHS TSLFPKVEPF LPGHYEVLDL KPSGKVVSVE 200
    VVKFHSYKDE PLHAACDTVG NLPSGFDLET VKSNIRVLFE NAVRKRLMAH 250
    RRIGCLLSGG LDSSLVAAVL LKLMKEMNIK YPLQTFAIGM ENSPDLLAAR 300
    KVAAHIGSEH HEVIFNSEEG IQAVEEVIFS LETYDITTVR ASIGMYLVSK 350
    YIRKKTDSVV IFSGEGSDEL TQGYIYFHKA PSPEEAAEES ERLLKELYLF 400
    DVLRADRTTA AHGLELRVPF LDHRFTSYYL SLPAELRIPK NGIEKYLLRQ 450
    SFEDSNLLPK EILWRPKEAF SDGIASVKKS WFSILQDYID QQVDDLLLEK 500
    AAEKYPFNPP RTKESYYYRQ IFEKHYPGRS SWLPHYWMPR WVEATDPSAR 550
    TLKHYKSAIQ E 561
    Length:561
    Mass (Da):64,097
    Last modified:January 23, 2007 - v3
    Checksum:i94EFE934BED58FBF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720341 mRNA. Translation: CAG32000.1.
    RefSeqiNP_001026148.1. NM_001030977.1.
    UniGeneiGga.22299.

    Genome annotation databases

    GeneIDi420574.
    KEGGigga:420574.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720341 mRNA. Translation: CAG32000.1 .
    RefSeqi NP_001026148.1. NM_001030977.1.
    UniGenei Gga.22299.

    3D structure databases

    ProteinModelPortali Q5ZJU3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 681517. 1 interaction.
    STRINGi 9031.ENSGALP00000015846.

    Proteomic databases

    PaxDbi Q5ZJU3.
    PRIDEi Q5ZJU3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 420574.
    KEGGi gga:420574.

    Organism-specific databases

    CTDi 440.

    Phylogenomic databases

    eggNOGi COG0367.
    HOGENOMi HOG000027493.
    HOVERGENi HBG003103.
    InParanoidi Q5ZJU3.
    KOi K01953.
    PhylomeDBi Q5ZJU3.

    Enzyme and pathway databases

    UniPathwayi UPA00134 ; UER00195 .

    Miscellaneous databases

    NextBioi 20823466.
    PROi Q5ZJU3.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR006426. Asn_synth_AEB.
    IPR001962. Asn_synthase.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    Pfami PF00733. Asn_synthase. 1 hit.
    PF13537. GATase_7. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
    SUPFAMi SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: CB.
      Tissue: Bursa of Fabricius.

    Entry informationi

    Entry nameiASNS_CHICK
    AccessioniPrimary (citable) accession number: Q5ZJU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 70 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3