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Q5ZJU3

- ASNS_CHICK

UniProt

Q5ZJU3 - ASNS_CHICK

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Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

ASNS

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei97 – 971GlutamineBy similarity
Binding sitei256 – 2561ATP; via carbonyl oxygenBy similarity
Binding sitei288 – 2881ATP; via amide nitrogen and carbonyl oxygenBy similarity
Sitei365 – 3651Important for beta-aspartyl-AMP intermediate formationBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi363 – 3642ATPBy similarity

GO - Molecular functioni

  1. asparagine synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. L-asparagine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:ASNS
ORF Names:RCJMB04_15l3
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 561560Asparagine synthetase [glutamine-hydrolyzing]PRO_0000056915Add
BLAST

Proteomic databases

PaxDbiQ5ZJU3.
PRIDEiQ5ZJU3.

Interactioni

Protein-protein interaction databases

BioGridi681517. 1 interaction.
STRINGi9031.ENSGALP00000015846.

Structurei

3D structure databases

ProteinModelPortaliQ5ZJU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 191190Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini213 – 536324Asparagine synthetaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 535Glutamine bindingBy similarity
Regioni75 – 773Glutamine bindingBy similarity

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0367.
HOGENOMiHOG000027493.
HOVERGENiHBG003103.
InParanoidiQ5ZJU3.
KOiK01953.
PhylomeDBiQ5ZJU3.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFiPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5ZJU3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIWALFGS DECLSVQCLS AMKIAHRGPD AFRFENVNGF TNCCFGFHRL
60 70 80 90 100
AVVDQLYGMQ PIRVKKFPYL WLCYNGEIYN FKQLQEQFGF EYQTLVDGEV
110 120 130 140 150
ILHLYNRGGI EQTASMLDGV FAFILLDTAN RKVFLARDTY GVRPLFKVLT
160 170 180 190 200
DDGFLGVCSE AKGLINLKHS TSLFPKVEPF LPGHYEVLDL KPSGKVVSVE
210 220 230 240 250
VVKFHSYKDE PLHAACDTVG NLPSGFDLET VKSNIRVLFE NAVRKRLMAH
260 270 280 290 300
RRIGCLLSGG LDSSLVAAVL LKLMKEMNIK YPLQTFAIGM ENSPDLLAAR
310 320 330 340 350
KVAAHIGSEH HEVIFNSEEG IQAVEEVIFS LETYDITTVR ASIGMYLVSK
360 370 380 390 400
YIRKKTDSVV IFSGEGSDEL TQGYIYFHKA PSPEEAAEES ERLLKELYLF
410 420 430 440 450
DVLRADRTTA AHGLELRVPF LDHRFTSYYL SLPAELRIPK NGIEKYLLRQ
460 470 480 490 500
SFEDSNLLPK EILWRPKEAF SDGIASVKKS WFSILQDYID QQVDDLLLEK
510 520 530 540 550
AAEKYPFNPP RTKESYYYRQ IFEKHYPGRS SWLPHYWMPR WVEATDPSAR
560
TLKHYKSAIQ E
Length:561
Mass (Da):64,097
Last modified:January 23, 2007 - v3
Checksum:i94EFE934BED58FBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720341 mRNA. Translation: CAG32000.1.
RefSeqiNP_001026148.1. NM_001030977.1.
UniGeneiGga.22299.

Genome annotation databases

GeneIDi420574.
KEGGigga:420574.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720341 mRNA. Translation: CAG32000.1 .
RefSeqi NP_001026148.1. NM_001030977.1.
UniGenei Gga.22299.

3D structure databases

ProteinModelPortali Q5ZJU3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 681517. 1 interaction.
STRINGi 9031.ENSGALP00000015846.

Proteomic databases

PaxDbi Q5ZJU3.
PRIDEi Q5ZJU3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 420574.
KEGGi gga:420574.

Organism-specific databases

CTDi 440.

Phylogenomic databases

eggNOGi COG0367.
HOGENOMi HOG000027493.
HOVERGENi HBG003103.
InParanoidi Q5ZJU3.
KOi K01953.
PhylomeDBi Q5ZJU3.

Enzyme and pathway databases

UniPathwayi UPA00134 ; UER00195 .

Miscellaneous databases

NextBioi 20823466.
PROi Q5ZJU3.

Family and domain databases

Gene3Di 3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
Pfami PF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view ]
PIRSFi PIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMi SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01536. asn_synth_AEB. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiASNS_CHICK
AccessioniPrimary (citable) accession number: Q5ZJU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 71 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3