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Protein

Asparagine synthetase [glutamine-hydrolyzing]

Gene

ASNS

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] (ASNS)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei97GlutamineBy similarity1
Binding sitei256ATP; via carbonyl oxygenBy similarity1
Binding sitei288ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Sitei365Important for beta-aspartyl-AMP intermediate formationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi363 – 364ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] (EC:6.3.5.4)
Alternative name(s):
Glutamine-dependent asparagine synthetase
Gene namesi
Name:ASNS
ORF Names:RCJMB04_15l3
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569152 – 561Asparagine synthetase [glutamine-hydrolyzing]Add BLAST560

Proteomic databases

PaxDbiQ5ZJU3.
PRIDEiQ5ZJU3.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi681517. 1 interactor.
STRINGi9031.ENSGALP00000015846.

Structurei

3D structure databases

ProteinModelPortaliQ5ZJU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 191Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST190
Domaini213 – 536Asparagine synthetaseAdd BLAST324

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 53Glutamine bindingBy similarity5
Regioni75 – 77Glutamine bindingBy similarity3

Sequence similaritiesi

Contains 1 asparagine synthetase domain.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG0571. Eukaryota.
COG0367. LUCA.
HOGENOMiHOG000027493.
HOVERGENiHBG003103.
InParanoidiQ5ZJU3.
KOiK01953.
PhylomeDBiQ5ZJU3.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 2 hits.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5ZJU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIWALFGS DECLSVQCLS AMKIAHRGPD AFRFENVNGF TNCCFGFHRL
60 70 80 90 100
AVVDQLYGMQ PIRVKKFPYL WLCYNGEIYN FKQLQEQFGF EYQTLVDGEV
110 120 130 140 150
ILHLYNRGGI EQTASMLDGV FAFILLDTAN RKVFLARDTY GVRPLFKVLT
160 170 180 190 200
DDGFLGVCSE AKGLINLKHS TSLFPKVEPF LPGHYEVLDL KPSGKVVSVE
210 220 230 240 250
VVKFHSYKDE PLHAACDTVG NLPSGFDLET VKSNIRVLFE NAVRKRLMAH
260 270 280 290 300
RRIGCLLSGG LDSSLVAAVL LKLMKEMNIK YPLQTFAIGM ENSPDLLAAR
310 320 330 340 350
KVAAHIGSEH HEVIFNSEEG IQAVEEVIFS LETYDITTVR ASIGMYLVSK
360 370 380 390 400
YIRKKTDSVV IFSGEGSDEL TQGYIYFHKA PSPEEAAEES ERLLKELYLF
410 420 430 440 450
DVLRADRTTA AHGLELRVPF LDHRFTSYYL SLPAELRIPK NGIEKYLLRQ
460 470 480 490 500
SFEDSNLLPK EILWRPKEAF SDGIASVKKS WFSILQDYID QQVDDLLLEK
510 520 530 540 550
AAEKYPFNPP RTKESYYYRQ IFEKHYPGRS SWLPHYWMPR WVEATDPSAR
560
TLKHYKSAIQ E
Length:561
Mass (Da):64,097
Last modified:January 23, 2007 - v3
Checksum:i94EFE934BED58FBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720341 mRNA. Translation: CAG32000.1.
RefSeqiNP_001026148.1. NM_001030977.1.
UniGeneiGga.22299.

Genome annotation databases

GeneIDi420574.
KEGGigga:420574.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720341 mRNA. Translation: CAG32000.1.
RefSeqiNP_001026148.1. NM_001030977.1.
UniGeneiGga.22299.

3D structure databases

ProteinModelPortaliQ5ZJU3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi681517. 1 interactor.
STRINGi9031.ENSGALP00000015846.

Protein family/group databases

MEROPSiC44.001.

Proteomic databases

PaxDbiQ5ZJU3.
PRIDEiQ5ZJU3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi420574.
KEGGigga:420574.

Organism-specific databases

CTDi440.

Phylogenomic databases

eggNOGiKOG0571. Eukaryota.
COG0367. LUCA.
HOGENOMiHOG000027493.
HOVERGENiHBG003103.
InParanoidiQ5ZJU3.
KOiK01953.
PhylomeDBiQ5ZJU3.

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.

Miscellaneous databases

PROiQ5ZJU3.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 2 hits.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASNS_CHICK
AccessioniPrimary (citable) accession number: Q5ZJU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 79 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.