Q5ZJN4 (UBP10_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 10
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 10
Ubiquitin thioesterase 10
Ubiquitin-specific-processing protease 10

Gene names

Name:	USP10
ORF Names:	RCJMB04_16o18

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	785 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteracts MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase C19 family. USP10 subfamily.

Ontologies

Keywords
Biological process	Autophagy DNA damage DNA repair Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage response, signal transduction by p53 class mediator Inferred from sequence or structural similarity. Source: UniProtKB DNA repair Inferred from electronic annotation. Source: UniProtKB-KW autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB regulation of autophagy Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	early endosome Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cysteine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB ion channel binding Inferred from sequence or structural similarity. Source: UniProtKB p53 binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 785	785	Ubiquitin carboxyl-terminal hydrolase 10		PRO_0000393001
Sites
	Active site	410	1	Nucleophile By similarity
	Active site	736	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZJN4 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 97AB9EAE92AE32AB	FASTA	785	86,556
	10 20 30 40 50 60 MAVNGAQYIF GEFSPDEFNQ FFVTPRCSVE LPPYNETVSC GIKSTNEEYQ RIEFGVNEVI 70 80 90 100 110 120 ETESSVLNNT DYSISSTLNP QAPEFILSCA PAQKTPDETN YNSIDCQFSD PTLTLDSGSN 130 140 150 160 170 180 AENDGLSGGL GQRERKKKKK RPPGYYSYLE DVSDGVAPTE ALVNGHANSS GLNSIGTEDT 190 200 210 220 230 240 ELTGDIPSLA TPRTCNSPDN SVDFVHEAVS DDSVSSALDN TRTAGQPEVC RVTNSEQFCI 250 260 270 280 290 300 PSETGRDSPL RTAVVQPYAG TDTTESLGVT NGQTLESSGE DTAANGVELH TVESTDSDQA 310 320 330 340 350 360 KPEEASPTTE ATATVAGSVP VNQPAKSWAS LFHNSKPSAS TSVVYVETKY TPPATSTLVP 370 380 390 400 410 420 EKQVEVKEGP VPVSEDPVAI KIAELLENVK LVHKPVSLQP RGLINKGNWC YINATLQALV 430 440 450 460 470 480 ACPPMYHLMK SIPMYSKSQR PCTSTPMIDS FVRLMNEFTN MPVPPKAKQA LGDKIVRDIR 490 500 510 520 530 540 PGAAFEPTYI YRLLTVIKSS LSEKGRQEDA EEYLGFILNG LHEEMLTLKK LLSPHNEKLS 550 560 570 580 590 600 VSNGPEVQTV REEEEQDEQG EGSEDEWEQV GPRNKSSVTR QADFVQTPIT DIFGGHIRSV 610 620 630 640 650 660 VYQQSSKESA TLQPFFTLQL DIQSDKIRTV QDALESLVAR ESVQGYTTKT KQEVEISRRV 670 680 690 700 710 720 TLEELPPVLV LHLKRFVYEK TGGCQKLIKN IEYPVDLEIS KELLSPGVKS KIFKGQRTYR 730 740 750 760 770 780 LFAVVYHHGN SATGDHYTTD VFQIGLNGWL RIDDQAVKVI NQYQVVKPSA ERTAYLLYYR RVDLL

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ720400 mRNA. Translation: CAG32059.1.
IPI	IPI00819631.
RefSeq	NP_001006130.1. NM_001006130.1.
UniGene	Gga.3193.
3D structure databases
ProteinModelPortal	Q5ZJN4.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000038252.
Protein family/group databases
MEROPS	C19.018.
Proteomic databases
PaxDb	Q5ZJN4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	415817.
KEGG	gga:415817.
Organism-specific databases
CTD	9100.
Phylogenomic databases
eggNOG	COG5533.
HOGENOM	HOG000285959.
HOVERGEN	HBG059823.
InParanoid	Q5ZJN4.
KO	K11841.
OrthoDB	EOG4FN4H7.
Family and domain databases
InterPro	IPR009818. Ataxin-2_C. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF07145. PAM2. 1 hit. PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20819362.

Entry information

Entry name

UBP10_CHICK

Accession

Primary (citable) accession number: Q5ZJN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program