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Q5ZJL9 (SAMH1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Short name=dNTPase
EC=3.1.5.-
Gene names
Name:SAMHD1
ORF Names:RCJMB04_17d8
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length614 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Host restriction nuclease that blocks early-stage virus replication. May function by reducing the cellular dNTP levels to levels too low for viral reverse transcription to occur By similarity.

Catalytic activity

dNTP + H2O = Deoxynucleoside + triphosphate.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Enzyme regulation

Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP By similarity.

Subunit structure

Homodimer. Homotetramer; in dGTP-bound form By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the SAMHD1 family.

Contains 1 HD domain.

Contains 1 SAM (sterile alpha motif) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 614614Deoxynucleoside triphosphate triphosphohydrolase SAMHD1
PRO_0000361969

Regions

Domain37 – 10266SAM
Domain155 – 310156HD
Nucleotide binding128 – 1369dGTP By similarity
Nucleotide binding128 – 1369dGTP 1 By similarity
Nucleotide binding343 – 3453dGTP 2 By similarity
Region300 – 3067Substrate binding By similarity
Region361 – 3666Substrate binding By similarity

Sites

Active site2241 By similarity
Metal binding1581Zinc; via tele nitrogen By similarity
Metal binding1971Zinc; via tele nitrogen By similarity
Metal binding1981Zinc By similarity
Metal binding3021Zinc By similarity
Binding site1071dGTP 1 By similarity
Binding site1101dGTP 2; via amide nitrogen; shared with neighboring subunit By similarity
Binding site1401Substrate By similarity
Binding site1551Substrate By similarity
Binding site2011Substrate By similarity
Binding site3061Substrate By similarity
Binding site3101Substrate By similarity
Binding site3241dGTP 2 By similarity
Binding site3491dGTP 2 By similarity
Binding site3571Substrate By similarity
Binding site3671dGTP 2; shared with neighboring subunit By similarity
Binding site3681dGTP 2; shared with neighboring subunit By similarity
Binding site4421dGTP 1; shared with neighboring subunit By similarity
Binding site4461dGTP 1; shared with neighboring subunit By similarity
Binding site5151dGTP 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5ZJL9 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 42A1CE5238A4D056

FASTA61470,220
        10         20         30         40         50         60 
MGSPAAGWGA APAKRARREG SAESSCGSPA DRDPRLWDTE RLCQHLSRNG VGEPGLLRRF 

        70         80         90        100        110        120 
RESGVTGAML LDLPACALRI THACLPDERL EVLACLNQLR QTADIMKVFN DPVHGHIEIH 

       130        140        150        160        170        180 
PLLVRIIDTP QFQRLRYIKQ LGGTYFVFPG ASHNRFEHSL GVGYLAGCLV RELKERQPEL 

       190        200        210        220        230        240 
DITQRDILCV EIAGLCHDLG HGPFSHMFDG RFIPLARQGL NWKHETASVE MFEHLITSNK 

       250        260        270        280        290        300 
LEEIMESYGL ILEEDIAFIK EQIGGPIDET ACEESWPYRG RPKEKSFLYE IVANKKNGID 

       310        320        330        340        350        360 
VDKWDYFARD CHHLGIQNNF DYRRLIKFTR VCEAGNQKHI CARDKEVGNL YDMFHTRNCL 

       370        380        390        400        410        420 
HRRAYQHKVG NIIEIMITEA FQKADCFFQI EGSKGKLYHI STAMEDMEAY TKLTDNIYLE 

       430        440        450        460        470        480 
ILHSSRPELS EAREILHKIE RRELYKFLGE TQPEKVNEIP KDEYDGLAGD IANSKPEKDP 

       490        500        510        520        530        540 
PDVELTAEDF IVDVVNMDYG MKDQNPIDNV LFYCKADPSK AIKVSKEQVS RLLPGTFSEQ 

       550        560        570        580        590        600 
VIRVYCKRQD PIIVSAAKQY FVQWCIKRDF TKPQDGDVVA PHLTPLKQSW NNRSKTEYTT 

       610 
ASEPSCKQKL SFNK 

« Hide

References

[1]"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis."
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.
Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: CB.
Tissue: Bursa of Fabricius.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ720415 mRNA. Translation: CAG32074.1.
RefSeqNP_001026016.1. NM_001030845.1.
UniGeneGga.20678.

3D structure databases

ProteinModelPortalQ5ZJL9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000001866.

Proteomic databases

PaxDbQ5ZJL9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID419125.
KEGGgga:419125.

Organism-specific databases

CTD25939.

Phylogenomic databases

eggNOGCOG1078.
HOGENOMHOG000264286.
HOVERGENHBG054208.
InParanoidQ5ZJL9.
PhylomeDBQ5ZJL9.

Family and domain databases

Gene3D1.10.3210.10. 2 hits.
InterProIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view]
PfamPF01966. HD. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20822229.
PROQ5ZJL9.

Entry information

Entry nameSAMH1_CHICK
AccessionPrimary (citable) accession number: Q5ZJL9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families