Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5ZJL9

- SAMH1_CHICK

UniProt

Q5ZJL9 - SAMH1_CHICK

Protein

Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Gene

SAMHD1

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Host restriction nuclease that blocks early-stage virus replication. May function by reducing the cellular dNTP levels to levels too low for viral reverse transcription to occur By similarity.By similarity

    Catalytic activityi

    dNTP + H2O = Deoxynucleoside + triphosphate.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Enzyme regulationi

    Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei107 – 1071dGTP 1By similarity
    Binding sitei110 – 1101dGTP 2; via amide nitrogen; shared with neighboring subunitBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Binding sitei155 – 1551SubstrateBy similarity
    Metal bindingi158 – 1581Zinc; via tele nitrogenBy similarity
    Metal bindingi197 – 1971Zinc; via tele nitrogenBy similarity
    Metal bindingi198 – 1981ZincBy similarity
    Binding sitei201 – 2011SubstrateBy similarity
    Active sitei224 – 2241By similarity
    Metal bindingi302 – 3021ZincBy similarity
    Binding sitei306 – 3061SubstrateBy similarity
    Binding sitei310 – 3101SubstrateBy similarity
    Binding sitei324 – 3241dGTP 2By similarity
    Binding sitei349 – 3491dGTP 2By similarity
    Binding sitei357 – 3571SubstrateBy similarity
    Binding sitei367 – 3671dGTP 2; shared with neighboring subunitBy similarity
    Binding sitei368 – 3681dGTP 2; shared with neighboring subunitBy similarity
    Binding sitei442 – 4421dGTP 1; shared with neighboring subunitBy similarity
    Binding sitei446 – 4461dGTP 1; shared with neighboring subunitBy similarity
    Binding sitei515 – 5151dGTP 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi128 – 1369dGTPBy similarity
    Nucleotide bindingi128 – 1369dGTP 1By similarity
    Nucleotide bindingi343 – 3453dGTP 2By similarity

    GO - Molecular functioni

    1. dGTPase activity Source: UniProtKB
    2. dGTP binding Source: UniProtKB
    3. nucleic acid binding Source: UniProtKB
    4. phosphoric diester hydrolase activity Source: InterPro
    5. RNA binding Source: UniProtKB
    6. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. dATP catabolic process Source: UniProtKB
    2. defense response to virus Source: UniProtKB
    3. dGTP catabolic process Source: UniProtKB
    4. innate immune response Source: UniProtKB-KW
    5. regulation of innate immune response Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antiviral defense, Immunity, Innate immunity

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (EC:3.1.5.-)
    Short name:
    dNTPase
    Gene namesi
    Name:SAMHD1
    ORF Names:RCJMB04_17d8
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 614614Deoxynucleoside triphosphate triphosphohydrolase SAMHD1PRO_0000361969Add
    BLAST

    Proteomic databases

    PaxDbiQ5ZJL9.

    Interactioni

    Subunit structurei

    Homodimer. Homotetramer; in dGTP-bound form By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000001866.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5ZJL9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 10266SAMAdd
    BLAST
    Domaini155 – 310156HDAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni300 – 3067Substrate bindingBy similarity
    Regioni361 – 3666Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the SAMHD1 family.Curated
    Contains 1 HD domain.Curated

    Phylogenomic databases

    eggNOGiCOG1078.
    HOGENOMiHOG000264286.
    HOVERGENiHBG054208.
    InParanoidiQ5ZJL9.
    PhylomeDBiQ5ZJL9.

    Family and domain databases

    Gene3Di1.10.3210.10. 2 hits.
    InterProiIPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    [Graphical view]
    PfamiPF01966. HD. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5ZJL9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSPAAGWGA APAKRARREG SAESSCGSPA DRDPRLWDTE RLCQHLSRNG    50
    VGEPGLLRRF RESGVTGAML LDLPACALRI THACLPDERL EVLACLNQLR 100
    QTADIMKVFN DPVHGHIEIH PLLVRIIDTP QFQRLRYIKQ LGGTYFVFPG 150
    ASHNRFEHSL GVGYLAGCLV RELKERQPEL DITQRDILCV EIAGLCHDLG 200
    HGPFSHMFDG RFIPLARQGL NWKHETASVE MFEHLITSNK LEEIMESYGL 250
    ILEEDIAFIK EQIGGPIDET ACEESWPYRG RPKEKSFLYE IVANKKNGID 300
    VDKWDYFARD CHHLGIQNNF DYRRLIKFTR VCEAGNQKHI CARDKEVGNL 350
    YDMFHTRNCL HRRAYQHKVG NIIEIMITEA FQKADCFFQI EGSKGKLYHI 400
    STAMEDMEAY TKLTDNIYLE ILHSSRPELS EAREILHKIE RRELYKFLGE 450
    TQPEKVNEIP KDEYDGLAGD IANSKPEKDP PDVELTAEDF IVDVVNMDYG 500
    MKDQNPIDNV LFYCKADPSK AIKVSKEQVS RLLPGTFSEQ VIRVYCKRQD 550
    PIIVSAAKQY FVQWCIKRDF TKPQDGDVVA PHLTPLKQSW NNRSKTEYTT 600
    ASEPSCKQKL SFNK 614
    Length:614
    Mass (Da):70,220
    Last modified:November 23, 2004 - v1
    Checksum:i42A1CE5238A4D056
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720415 mRNA. Translation: CAG32074.1.
    RefSeqiNP_001026016.1. NM_001030845.1.
    UniGeneiGga.20678.

    Genome annotation databases

    GeneIDi419125.
    KEGGigga:419125.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720415 mRNA. Translation: CAG32074.1 .
    RefSeqi NP_001026016.1. NM_001030845.1.
    UniGenei Gga.20678.

    3D structure databases

    ProteinModelPortali Q5ZJL9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000001866.

    Proteomic databases

    PaxDbi Q5ZJL9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 419125.
    KEGGi gga:419125.

    Organism-specific databases

    CTDi 25939.

    Phylogenomic databases

    eggNOGi COG1078.
    HOGENOMi HOG000264286.
    HOVERGENi HBG054208.
    InParanoidi Q5ZJL9.
    PhylomeDBi Q5ZJL9.

    Miscellaneous databases

    NextBioi 20822229.
    PROi Q5ZJL9.

    Family and domain databases

    Gene3Di 1.10.3210.10. 2 hits.
    InterProi IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    [Graphical view ]
    Pfami PF01966. HD. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: CB.
      Tissue: Bursa of Fabricius.

    Entry informationi

    Entry nameiSAMH1_CHICK
    AccessioniPrimary (citable) accession number: Q5ZJL9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3