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Q5ZJL9

- SAMH1_CHICK

UniProt

Q5ZJL9 - SAMH1_CHICK

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Protein

Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Gene
SAMHD1, RCJMB04_17d8
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Host restriction nuclease that blocks early-stage virus replication. May function by reducing the cellular dNTP levels to levels too low for viral reverse transcription to occur By similarity.

Catalytic activityi

dNTP + H2O = Deoxynucleoside + triphosphate.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Enzyme regulationi

Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071dGTP 1 By similarity
Binding sitei110 – 1101dGTP 2; via amide nitrogen; shared with neighboring subunit By similarity
Binding sitei140 – 1401Substrate By similarity
Binding sitei155 – 1551Substrate By similarity
Metal bindingi158 – 1581Zinc; via tele nitrogen By similarity
Metal bindingi197 – 1971Zinc; via tele nitrogen By similarity
Metal bindingi198 – 1981Zinc By similarity
Binding sitei201 – 2011Substrate By similarity
Active sitei224 – 2241 By similarity
Metal bindingi302 – 3021Zinc By similarity
Binding sitei306 – 3061Substrate By similarity
Binding sitei310 – 3101Substrate By similarity
Binding sitei324 – 3241dGTP 2 By similarity
Binding sitei349 – 3491dGTP 2 By similarity
Binding sitei357 – 3571Substrate By similarity
Binding sitei367 – 3671dGTP 2; shared with neighboring subunit By similarity
Binding sitei368 – 3681dGTP 2; shared with neighboring subunit By similarity
Binding sitei442 – 4421dGTP 1; shared with neighboring subunit By similarity
Binding sitei446 – 4461dGTP 1; shared with neighboring subunit By similarity
Binding sitei515 – 5151dGTP 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi128 – 1369dGTP By similarity
Nucleotide bindingi128 – 1369dGTP 1 By similarity
Nucleotide bindingi343 – 3453dGTP 2 By similarity

GO - Molecular functioni

  1. dGTPase activity Source: UniProtKB
  2. dGTP binding Source: UniProtKB
  3. nucleic acid binding Source: UniProtKB
  4. phosphoric diester hydrolase activity Source: InterPro
  5. RNA binding Source: UniProtKB
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. dATP catabolic process Source: UniProtKB
  2. defense response to virus Source: UniProtKB
  3. dGTP catabolic process Source: UniProtKB
  4. innate immune response Source: UniProtKB-KW
  5. regulation of innate immune response Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (EC:3.1.5.-)
Short name:
dNTPase
Gene namesi
Name:SAMHD1
ORF Names:RCJMB04_17d8
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 614614Deoxynucleoside triphosphate triphosphohydrolase SAMHD1PRO_0000361969Add
BLAST

Proteomic databases

PaxDbiQ5ZJL9.

Interactioni

Subunit structurei

Homodimer. Homotetramer; in dGTP-bound form By similarity.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000001866.

Structurei

3D structure databases

ProteinModelPortaliQ5ZJL9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 10266SAMAdd
BLAST
Domaini155 – 310156HDAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni300 – 3067Substrate binding By similarity
Regioni361 – 3666Substrate binding By similarity

Sequence similaritiesi

Belongs to the SAMHD1 family.
Contains 1 HD domain.

Phylogenomic databases

eggNOGiCOG1078.
HOGENOMiHOG000264286.
HOVERGENiHBG054208.
InParanoidiQ5ZJL9.
PhylomeDBiQ5ZJL9.

Family and domain databases

Gene3Di1.10.3210.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view]
PfamiPF01966. HD. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZJL9-1 [UniParc]FASTAAdd to Basket

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MGSPAAGWGA APAKRARREG SAESSCGSPA DRDPRLWDTE RLCQHLSRNG    50
VGEPGLLRRF RESGVTGAML LDLPACALRI THACLPDERL EVLACLNQLR 100
QTADIMKVFN DPVHGHIEIH PLLVRIIDTP QFQRLRYIKQ LGGTYFVFPG 150
ASHNRFEHSL GVGYLAGCLV RELKERQPEL DITQRDILCV EIAGLCHDLG 200
HGPFSHMFDG RFIPLARQGL NWKHETASVE MFEHLITSNK LEEIMESYGL 250
ILEEDIAFIK EQIGGPIDET ACEESWPYRG RPKEKSFLYE IVANKKNGID 300
VDKWDYFARD CHHLGIQNNF DYRRLIKFTR VCEAGNQKHI CARDKEVGNL 350
YDMFHTRNCL HRRAYQHKVG NIIEIMITEA FQKADCFFQI EGSKGKLYHI 400
STAMEDMEAY TKLTDNIYLE ILHSSRPELS EAREILHKIE RRELYKFLGE 450
TQPEKVNEIP KDEYDGLAGD IANSKPEKDP PDVELTAEDF IVDVVNMDYG 500
MKDQNPIDNV LFYCKADPSK AIKVSKEQVS RLLPGTFSEQ VIRVYCKRQD 550
PIIVSAAKQY FVQWCIKRDF TKPQDGDVVA PHLTPLKQSW NNRSKTEYTT 600
ASEPSCKQKL SFNK 614
Length:614
Mass (Da):70,220
Last modified:November 23, 2004 - v1
Checksum:i42A1CE5238A4D056
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ720415 mRNA. Translation: CAG32074.1.
RefSeqiNP_001026016.1. NM_001030845.1.
UniGeneiGga.20678.

Genome annotation databases

GeneIDi419125.
KEGGigga:419125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ720415 mRNA. Translation: CAG32074.1 .
RefSeqi NP_001026016.1. NM_001030845.1.
UniGenei Gga.20678.

3D structure databases

ProteinModelPortali Q5ZJL9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000001866.

Proteomic databases

PaxDbi Q5ZJL9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 419125.
KEGGi gga:419125.

Organism-specific databases

CTDi 25939.

Phylogenomic databases

eggNOGi COG1078.
HOGENOMi HOG000264286.
HOVERGENi HBG054208.
InParanoidi Q5ZJL9.
PhylomeDBi Q5ZJL9.

Miscellaneous databases

NextBioi 20822229.
PROi Q5ZJL9.

Family and domain databases

Gene3Di 1.10.3210.10. 2 hits.
InterProi IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view ]
Pfami PF01966. HD. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiSAMH1_CHICK
AccessioniPrimary (citable) accession number: Q5ZJL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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