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Protein

Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Gene

SAMHD1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Host restriction nuclease that blocks early-stage virus replication. May function by reducing the cellular dNTP levels to levels too low for viral reverse transcription to occur (By similarity).By similarity

Catalytic activityi

dNTP + H2O = Deoxynucleoside + triphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107dGTP 1By similarity1
Binding sitei110dGTP 2; via amide nitrogen; shared with neighboring subunitBy similarity1
Binding sitei140SubstrateBy similarity1
Binding sitei155SubstrateBy similarity1
Metal bindingi158Zinc; via tele nitrogenBy similarity1
Metal bindingi197Zinc; via tele nitrogenBy similarity1
Metal bindingi198ZincBy similarity1
Binding sitei201SubstrateBy similarity1
Active sitei224By similarity1
Metal bindingi302ZincBy similarity1
Binding sitei306SubstrateBy similarity1
Binding sitei310SubstrateBy similarity1
Binding sitei324dGTP 2By similarity1
Binding sitei349dGTP 2By similarity1
Binding sitei357SubstrateBy similarity1
Binding sitei367dGTP 2; shared with neighboring subunitBy similarity1
Binding sitei368dGTP 2; shared with neighboring subunitBy similarity1
Binding sitei442dGTP 1; shared with neighboring subunitBy similarity1
Binding sitei446dGTP 1; shared with neighboring subunitBy similarity1
Binding sitei515dGTP 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi128 – 136dGTPBy similarity9
Nucleotide bindingi128 – 136dGTP 1By similarity9
Nucleotide bindingi343 – 345dGTP 2By similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (EC:3.1.5.-)
Short name:
dNTPase
Gene namesi
Name:SAMHD1
ORF Names:RCJMB04_17d8
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003619691 – 614Deoxynucleoside triphosphate triphosphohydrolase SAMHD1Add BLAST614

Proteomic databases

PaxDbiQ5ZJL9.

Interactioni

Subunit structurei

Homodimer. Homotetramer; in dGTP-bound form (By similarity).By similarity

Protein-protein interaction databases

BioGridi680279. 1 interactor.
STRINGi9031.ENSGALP00000001866.

Structurei

3D structure databases

ProteinModelPortaliQ5ZJL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 102SAMAdd BLAST66
Domaini155 – 310HDAdd BLAST156

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni300 – 306Substrate bindingBy similarity7
Regioni361 – 366Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the SAMHD1 family.Curated
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiKOG2681. Eukaryota.
COG1078. LUCA.
HOGENOMiHOG000264286.
HOVERGENiHBG054208.
InParanoidiQ5ZJL9.
PhylomeDBiQ5ZJL9.

Family and domain databases

Gene3Di1.10.3210.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view]
PfamiPF01966. HD. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZJL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSPAAGWGA APAKRARREG SAESSCGSPA DRDPRLWDTE RLCQHLSRNG
60 70 80 90 100
VGEPGLLRRF RESGVTGAML LDLPACALRI THACLPDERL EVLACLNQLR
110 120 130 140 150
QTADIMKVFN DPVHGHIEIH PLLVRIIDTP QFQRLRYIKQ LGGTYFVFPG
160 170 180 190 200
ASHNRFEHSL GVGYLAGCLV RELKERQPEL DITQRDILCV EIAGLCHDLG
210 220 230 240 250
HGPFSHMFDG RFIPLARQGL NWKHETASVE MFEHLITSNK LEEIMESYGL
260 270 280 290 300
ILEEDIAFIK EQIGGPIDET ACEESWPYRG RPKEKSFLYE IVANKKNGID
310 320 330 340 350
VDKWDYFARD CHHLGIQNNF DYRRLIKFTR VCEAGNQKHI CARDKEVGNL
360 370 380 390 400
YDMFHTRNCL HRRAYQHKVG NIIEIMITEA FQKADCFFQI EGSKGKLYHI
410 420 430 440 450
STAMEDMEAY TKLTDNIYLE ILHSSRPELS EAREILHKIE RRELYKFLGE
460 470 480 490 500
TQPEKVNEIP KDEYDGLAGD IANSKPEKDP PDVELTAEDF IVDVVNMDYG
510 520 530 540 550
MKDQNPIDNV LFYCKADPSK AIKVSKEQVS RLLPGTFSEQ VIRVYCKRQD
560 570 580 590 600
PIIVSAAKQY FVQWCIKRDF TKPQDGDVVA PHLTPLKQSW NNRSKTEYTT
610
ASEPSCKQKL SFNK
Length:614
Mass (Da):70,220
Last modified:November 23, 2004 - v1
Checksum:i42A1CE5238A4D056
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720415 mRNA. Translation: CAG32074.1.
RefSeqiNP_001026016.1. NM_001030845.1.
UniGeneiGga.20678.

Genome annotation databases

GeneIDi419125.
KEGGigga:419125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720415 mRNA. Translation: CAG32074.1.
RefSeqiNP_001026016.1. NM_001030845.1.
UniGeneiGga.20678.

3D structure databases

ProteinModelPortaliQ5ZJL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi680279. 1 interactor.
STRINGi9031.ENSGALP00000001866.

Proteomic databases

PaxDbiQ5ZJL9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi419125.
KEGGigga:419125.

Organism-specific databases

CTDi25939.

Phylogenomic databases

eggNOGiKOG2681. Eukaryota.
COG1078. LUCA.
HOGENOMiHOG000264286.
HOVERGENiHBG054208.
InParanoidiQ5ZJL9.
PhylomeDBiQ5ZJL9.

Miscellaneous databases

PROiQ5ZJL9.

Family and domain databases

Gene3Di1.10.3210.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view]
PfamiPF01966. HD. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAMH1_CHICK
AccessioniPrimary (citable) accession number: Q5ZJL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 23, 2004
Last modified: June 8, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.