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Protein

Deoxynucleoside triphosphate triphosphohydrolase SAMHD1

Gene

SAMHD1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Host restriction nuclease that blocks early-stage virus replication. May function by reducing the cellular dNTP levels to levels too low for viral reverse transcription to occur (By similarity).By similarity

Catalytic activityi

dNTP + H2O = Deoxynucleoside + triphosphate.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Allosterically stimulated by dGTP which binds in a cleft at the interface of the homodimer and promotes the formation of highly active homotetramers. Each allosteric site binds two molecules of dGTP (dGTP1 and dGTP 2) between adjoining subunits. Not activated by dATP, dCTP and dTTP (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071dGTP 1By similarity
Binding sitei110 – 1101dGTP 2; via amide nitrogen; shared with neighboring subunitBy similarity
Binding sitei140 – 1401SubstrateBy similarity
Binding sitei155 – 1551SubstrateBy similarity
Metal bindingi158 – 1581Zinc; via tele nitrogenBy similarity
Metal bindingi197 – 1971Zinc; via tele nitrogenBy similarity
Metal bindingi198 – 1981ZincBy similarity
Binding sitei201 – 2011SubstrateBy similarity
Active sitei224 – 2241By similarity
Metal bindingi302 – 3021ZincBy similarity
Binding sitei306 – 3061SubstrateBy similarity
Binding sitei310 – 3101SubstrateBy similarity
Binding sitei324 – 3241dGTP 2By similarity
Binding sitei349 – 3491dGTP 2By similarity
Binding sitei357 – 3571SubstrateBy similarity
Binding sitei367 – 3671dGTP 2; shared with neighboring subunitBy similarity
Binding sitei368 – 3681dGTP 2; shared with neighboring subunitBy similarity
Binding sitei442 – 4421dGTP 1; shared with neighboring subunitBy similarity
Binding sitei446 – 4461dGTP 1; shared with neighboring subunitBy similarity
Binding sitei515 – 5151dGTP 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi128 – 1369dGTPBy similarity
Nucleotide bindingi128 – 1369dGTP 1By similarity
Nucleotide bindingi343 – 3453dGTP 2By similarity

GO - Molecular functioni

  1. dGTPase activity Source: UniProtKB
  2. dGTP binding Source: UniProtKB
  3. nucleic acid binding Source: UniProtKB
  4. RNA binding Source: UniProtKB
  5. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. dATP catabolic process Source: UniProtKB
  2. defense response to virus Source: UniProtKB
  3. dGTP catabolic process Source: UniProtKB
  4. innate immune response Source: UniProtKB-KW
  5. regulation of innate immune response Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antiviral defense, Immunity, Innate immunity

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 (EC:3.1.5.-)
Short name:
dNTPase
Gene namesi
Name:SAMHD1
ORF Names:RCJMB04_17d8
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

  1. Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 614614Deoxynucleoside triphosphate triphosphohydrolase SAMHD1PRO_0000361969Add
BLAST

Proteomic databases

PaxDbiQ5ZJL9.

Interactioni

Subunit structurei

Homodimer. Homotetramer; in dGTP-bound form (By similarity).By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000001866.

Structurei

3D structure databases

ProteinModelPortaliQ5ZJL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 10266SAMAdd
BLAST
Domaini155 – 310156HDAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni300 – 3067Substrate bindingBy similarity
Regioni361 – 3666Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the SAMHD1 family.Curated
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiCOG1078.
HOGENOMiHOG000264286.
HOVERGENiHBG054208.
InParanoidiQ5ZJL9.
PhylomeDBiQ5ZJL9.

Family and domain databases

Gene3Di1.10.3210.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view]
PfamiPF01966. HD. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZJL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSPAAGWGA APAKRARREG SAESSCGSPA DRDPRLWDTE RLCQHLSRNG
60 70 80 90 100
VGEPGLLRRF RESGVTGAML LDLPACALRI THACLPDERL EVLACLNQLR
110 120 130 140 150
QTADIMKVFN DPVHGHIEIH PLLVRIIDTP QFQRLRYIKQ LGGTYFVFPG
160 170 180 190 200
ASHNRFEHSL GVGYLAGCLV RELKERQPEL DITQRDILCV EIAGLCHDLG
210 220 230 240 250
HGPFSHMFDG RFIPLARQGL NWKHETASVE MFEHLITSNK LEEIMESYGL
260 270 280 290 300
ILEEDIAFIK EQIGGPIDET ACEESWPYRG RPKEKSFLYE IVANKKNGID
310 320 330 340 350
VDKWDYFARD CHHLGIQNNF DYRRLIKFTR VCEAGNQKHI CARDKEVGNL
360 370 380 390 400
YDMFHTRNCL HRRAYQHKVG NIIEIMITEA FQKADCFFQI EGSKGKLYHI
410 420 430 440 450
STAMEDMEAY TKLTDNIYLE ILHSSRPELS EAREILHKIE RRELYKFLGE
460 470 480 490 500
TQPEKVNEIP KDEYDGLAGD IANSKPEKDP PDVELTAEDF IVDVVNMDYG
510 520 530 540 550
MKDQNPIDNV LFYCKADPSK AIKVSKEQVS RLLPGTFSEQ VIRVYCKRQD
560 570 580 590 600
PIIVSAAKQY FVQWCIKRDF TKPQDGDVVA PHLTPLKQSW NNRSKTEYTT
610
ASEPSCKQKL SFNK
Length:614
Mass (Da):70,220
Last modified:November 23, 2004 - v1
Checksum:i42A1CE5238A4D056
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720415 mRNA. Translation: CAG32074.1.
RefSeqiNP_001026016.1. NM_001030845.1.
UniGeneiGga.20678.

Genome annotation databases

GeneIDi419125.
KEGGigga:419125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720415 mRNA. Translation: CAG32074.1.
RefSeqiNP_001026016.1. NM_001030845.1.
UniGeneiGga.20678.

3D structure databases

ProteinModelPortaliQ5ZJL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000001866.

Proteomic databases

PaxDbiQ5ZJL9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi419125.
KEGGigga:419125.

Organism-specific databases

CTDi25939.

Phylogenomic databases

eggNOGiCOG1078.
HOGENOMiHOG000264286.
HOVERGENiHBG054208.
InParanoidiQ5ZJL9.
PhylomeDBiQ5ZJL9.

Miscellaneous databases

NextBioi20822229.
PROiQ5ZJL9.

Family and domain databases

Gene3Di1.10.3210.10. 2 hits.
InterProiIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
[Graphical view]
PfamiPF01966. HD. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiSAMH1_CHICK
AccessioniPrimary (citable) accession number: Q5ZJL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 23, 2004
Last modified: February 4, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.