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Q5ZJL4 (CLP1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyribonucleotide 5'-hydroxyl-kinase Clp1
EC=2.7.1.78
Alternative name(s):
Polynucleotide kinase Clp1
Pre-mRNA cleavage complex II protein Clp1
Gene names
Name:CLP1
ORF Names:RCJMB04_17f4
OrganismGallus gallus (Chicken)
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing By similarity.

Catalytic activity

ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA.

ATP + 5'-dephospho-RNA = ADP + 5'-phospho-RNA.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the Clp1 family. Clp1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 425425Polyribonucleotide 5'-hydroxyl-kinase Clp1
PRO_0000375169

Regions

Nucleotide binding121 – 1288ATP Potential

Sequences

Sequence LengthMass (Da)Tools
Q5ZJL4 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 3464F8A1F7A5D235

FASTA42547,575
        10         20         30         40         50         60 
MADDGGDEKK QVAKFELERE TELRFEVEAS QTVQMELLTG MAEVFGTELT RNKKFTFDAG 

        70         80         90        100        110        120 
AKVAVFTWHG CTVQLSGRTE VAYVSKDTPM LLYLNTHTAL EQIRRQAERE DERGPRVMVV 

       130        140        150        160        170        180 
GPTDVGKSTV CRLLLNYAVR LGRRPTFVEL DVGQGSVSIP GTMGALYIER PADVEEGFSL 

       190        200        210        220        230        240 
QAPLVYHFGS TTPGTNIKLY NKITSRLADV FNQRCEVNRR ASVSGCVINT CGWVKGSGYQ 

       250        260        270        280        290        300 
ALVHAASAFE VDVVVVLDQE RLYNELKRDL PHFVRTVLLP KSGGVVERSK DFRRECRDDR 

       310        320        330        340        350        360 
IREYFYGFRG CFYPHAFDVK FSDVKIYKVG APTIPDSCLP LGMSQEDNQL KLVPVTPGRD 

       370        380        390        400        410        420 
MVHHLLSVSM ADSPDDNISE TSVAGFIVVT GVDLERQVFT VLSPAPRPLP KNFLLIMDIR 


FMDLK

« Hide

References

[1]"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis."
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.
Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: CB.
Tissue: Bursa of Fabricius.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ720420 mRNA. Translation: CAG32079.1.
IPIIPI00603182.
RefSeqNP_001012292.1. NM_001012292.1.
UniGeneGga.2011.

3D structure databases

ProteinModelPortalQ5ZJL4.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5ZJL4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID423131.
KEGGgga:423131.

Organism-specific databases

CTD10978.

Phylogenomic databases

eggNOGveNOG10989.
GeneTreeENSGT00390000000344.
HOGENOMHBG316814.
InParanoidQ5ZJL4.
OrthoDBEOG4K0QNF.
PhylomeDBQ5ZJL4.

Family and domain databases

InterProIPR010655. Pre-mRNA_cleavage_cplxII_Clp1.
[Graphical view]
KOK14399.
PfamPF06807. Clp1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCLP1_CHICK
AccessionPrimary (citable) accession number: Q5ZJL4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 23, 2004
Last modified: November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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