Q5ZJF4 (PRDX6_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxiredoxin-6
EC=1.11.1.15

Gene names

Name:	PRDX6
ORF Names:	RCJMB04_18k11

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in redox regulation of the cell. Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury By similarity.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity. Lysosome By similarity. Cytoplasmic vesicle By similarity.
Miscellaneous	The active site is the redox-active Cys-46 oxidized to Cys-SOH. Cys-SOH may rapidly react with a Cys-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin By similarity. Irreversibly inactivated by overoxidation of Cys-46 (to Cys-SO3H) upon oxidative stress By similarity.
Sequence similarities	Belongs to the AhpC/TSA family. Rehydrin subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Cytoplasm Cytoplasmic vesicle Lysosome
Domain	Redox-active center
Molecular function	Antioxidant Hydrolase Oxidoreductase Peroxidase
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 224	223	Peroxiredoxin-6		PRO_0000256863
Regions
	Domain	4 – 168	165	Thioredoxin
Sites
	Active site	31	1	For phospholipase activity By similarity
	Active site	46	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Modified residue	88	1	Phosphotyrosine By similarity
	Disulfide bond	46		Interchain; in linked form By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZJF4 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4F2759FB44EC9493	FASTA	224	24,977
	10 20 30 40 50 60 MPGLLLGDEA PNFEADTTQG GIRFHDFLGD SWGILFSHPR DFTPVCTTEL GRAAKLAPEF 70 80 90 100 110 120 SKRNVKMIAL SIDSVPDHLA WSKDINAYNG DQPVEKLPFP IIADKDRELA VKLGMLDPDE 130 140 150 160 170 180 RDKDGMPLTA RVVFIFGPDK KLKLSILYPA TTGRNFDEIL RVVDSLQLTA YKKVATPVDW 190 200 210 220 KCGDSVMVVP TLPDEEAKKL FPKGVFTKDL PSGKKYLRYT PQPE

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ720480 mRNA. Translation: CAG32139.1.
IPI	IPI00577013.
RefSeq	NP_001034418.1. NM_001039329.1.
UniGene	Gga.34325.
3D structure databases
HSSP	HSSP built from PDB template 1PRX based on UniProtKB P30041.
ProteinModelPortal	Q5ZJF4.
SMR	Q5ZJF4. Positions 4-223.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5ZJF4. 1 interaction.
STRING	Q5ZJF4.
Protein family/group databases
PeroxiBase	4421. Gga1CysPrx.
Proteomic databases
PRIDE	Q5ZJF4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	429062.
KEGG	gga:429062.
Organism-specific databases
CTD	9588.
Phylogenomic databases
eggNOG	veNOG13394.
GeneTree	ENSGT00550000074794.
HOGENOM	HBG493509.
HOVERGEN	HBG105234.
InParanoid	Q5ZJF4.
OrthoDB	EOG4M399H.
PhylomeDB	Q5ZJF4.
Family and domain databases
InterPro	IPR000866. AhpC/TSA. IPR024706. Peroxiredoxin_AhpC-typ. IPR019479. Peroxiredoxin_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K11188.
Pfam	PF10417. 1-cysPrx_C. 1 hit. PF00578. AhpC-TSA. 1 hit. [Graphical view]
PIRSF	PIRSF000239. AHPC. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRDX6_CHICK

Accession

Primary (citable) accession number: Q5ZJF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program