Q5ZJB4 (IKKA_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Short name=I kappa-B kinase alpha
Short name=IKK-A
Short name=IKK-alpha
Short name=IkBKA
Short name=IkappaB kinase
EC=2.7.11.10

Alternative name(s):
Conserved helix-loop-helix ubiquitous kinase
Nuclear factor NF-kappa-B inhibitor kinase alpha
Short name=NFKBIKA

Gene names

Name:	CHUK
Synonyms:	IKKA
ORF Names:	RCJMB04_19h23

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines By similarity.
Catalytic activity	ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].
Enzyme regulation	Activated when phosphorylated and inactivated when dephosphorylated By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Shuttles between the cytoplasm and the nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	MyD88-dependent toll-like receptor signaling pathway Traceable author statement. Source: Reactome activation of MAPK activity involved in innate immune response Traceable author statement. Source: Reactome activation of NF-kappaB-inducing kinase activity Traceable author statement. Source: Reactome toll-like receptor 15 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 2 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 21 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 3 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 4 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 5 signaling pathway Traceable author statement. Source: Reactome toll-like receptor 7 signaling pathway Traceable author statement. Source: Reactome
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW IkappaB kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	Inhibitor of nuclear factor kappa-B kinase subunit alpha		PRO_0000268160
Regions
	Domain	29 – 316	288	Protein kinase
	Domain	469 – 490	22	Leucine-zipper
	Nucleotide binding	35 – 43	9	ATP By similarity
	Region	753 – 758	6	NEMO-binding By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	58	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZJB4 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 135FDF10396B7B96	FASTA	759	86,144
	10 20 30 40 50 60 MERGAERGPP PAPGGVALRG QPAGGCGPWE MRDRLGTGGF GNVCLYQHQD TGARVAIKSC 70 80 90 100 110 120 RLELSVKNKD RWCHEIDIMK KLNHPNVVRA CEVPEEMNFL VNDVPLLAME YCSGGDLRKL 130 140 150 160 170 180 LNKPENCCGL KESQILSLLS DIGSGIQYLH ENRIIHRDLK PENIVLQDEG GKIIHKIIDL 190 200 210 220 230 240 GYAKDLDQGS LCTSFVGTLQ YLAPELFENK SYSVTVDYWS FGTMVFECIA GFRPFLHNLQ 250 260 270 280 290 300 PFTWHEKIKK KDPKHIFASE EMNGEVRFST HLPQPHSICS LIVEPMESWL QLMLNWDPEQ 310 320 330 340 350 360 RGGGLDPETN SPKCFLLMDH ILNLKIVHIL NMTSAKIVSF LLHPEESLHF LQNRIESETG 370 380 390 400 410 420 ISTGNQELLL ETGICLDPRK PASQCVIDGV RGWDSYMVYL FDKSKTVYDG PFASRSLSEC 430 440 450 460 470 480 VNYIVQDSKI QLPIPQLRKV WAEAVHYVIG LKEDYSRLFQ GQRAAMLSLL RYNANLIKMK 490 500 510 520 530 540 NNMVSASQQL KAKLEFFHQS IRLDLERYSD QMAYGISSEK MLKAWKEMEE KASHCAQAED 550 560 570 580 590 600 IGYLDEQIMA LHTEIVELQK SPYARRQGEV MESLEQRAID LYKQLKTRPP DHAYSDSTDM 610 620 630 640 650 660 VKIIVQTVQS QDRVLKELFG HLSKLLGCKQ KIIDLLPKIE VALNNIKEAD NSEMQMQGKR 670 680 690 700 710 720 QREIWHLLKI ACTQSSSRSL VSSSLEGTAS TPAATWVPQS SSEYAPHPLS SMATPGDGEN 730 740 750 FVDVIEENLN YLDRFSSMLQ EAREEQNNSL TNFDWSWLK

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ720520 mRNA. Translation: CAG32179.1.
IPI	IPI00586510.
RefSeq	NP_001012922.1. NM_001012904.1.
UniGene	Gga.5483.
3D structure databases
ProteinModelPortal	Q5ZJB4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZJB4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	423669.
KEGG	gga:423669.
Organism-specific databases
CTD	1147.
Phylogenomic databases
eggNOG	veNOG04904.
GeneTree	ENSGT00530000063051.
HOGENOM	HBG358635.
HOVERGEN	HBG018241.
InParanoid	Q5ZJB4.
OrthoDB	EOG4SJ5D7.
Enzyme and pathway databases
Reactome	REACT_25035. Innate immune response mediated by toll like receptors. REACT_75835. RLR (RIG-like receptor) mediated induction of IFN alpha/beta.
Family and domain databases
InterPro	IPR022007. IKKbetaNEMObind. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K04467.
Pfam	PF12179. IKKbetaNEMObind. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IKKA_CHICK

Accession

Primary (citable) accession number: Q5ZJB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program