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Protein

Inhibitor of nuclear factor kappa-B kinase subunit alpha

Gene

CHUK

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.By similarity

Catalytic activityi

ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

Enzyme regulationi

Activated when phosphorylated and inactivated when dephosphorylated.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58ATPPROSITE-ProRule annotation1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi35 – 43ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-1169091. Activation of NF-kappaB in B cells.
R-GGA-1227739. Caspase-8 and -10 mediated induction of NF-kB.
R-GGA-1227892. TRAF6 mediated NF-kB activation.
R-GGA-434001. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-GGA-434131. NFkB activation mediated by RIP1 complexed with activated TLR3.

Names & Taxonomyi

Protein namesi
Recommended name:
Inhibitor of nuclear factor kappa-B kinase subunit alpha (EC:2.7.11.10)
Short name:
I kappa-B kinase alpha
Short name:
IKK-A
Short name:
IKK-alpha
Short name:
IkBKA
Short name:
IkappaB kinase
Alternative name(s):
Conserved helix-loop-helix ubiquitous kinase
Nuclear factor NF-kappa-B inhibitor kinase alpha
Short name:
NFKBIKA
Gene namesi
Name:CHUK
Synonyms:IKKA
ORF Names:RCJMB04_19h23
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Shuttles between the cytoplasm and the nucleus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002681601 – 759Inhibitor of nuclear factor kappa-B kinase subunit alphaAdd BLAST759

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5ZJB4.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000005196.

Structurei

3D structure databases

ProteinModelPortaliQ5ZJB4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 316Protein kinasePROSITE-ProRule annotationAdd BLAST288

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni469 – 490Leucine-zipperAdd BLAST22
Regioni753 – 758NEMO-bindingBy similarity6

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4250. Eukaryota.
ENOG410XRMU. LUCA.
HOGENOMiHOG000038048.
HOVERGENiHBG018241.
InParanoidiQ5ZJB4.
KOiK04467.
PhylomeDBiQ5ZJB4.

Family and domain databases

InterProiIPR022007. IKKbetaNEMObind.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12179. IKKbetaNEMObind. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM01239. IKKbetaNEMObind. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZJB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERGAERGPP PAPGGVALRG QPAGGCGPWE MRDRLGTGGF GNVCLYQHQD
60 70 80 90 100
TGARVAIKSC RLELSVKNKD RWCHEIDIMK KLNHPNVVRA CEVPEEMNFL
110 120 130 140 150
VNDVPLLAME YCSGGDLRKL LNKPENCCGL KESQILSLLS DIGSGIQYLH
160 170 180 190 200
ENRIIHRDLK PENIVLQDEG GKIIHKIIDL GYAKDLDQGS LCTSFVGTLQ
210 220 230 240 250
YLAPELFENK SYSVTVDYWS FGTMVFECIA GFRPFLHNLQ PFTWHEKIKK
260 270 280 290 300
KDPKHIFASE EMNGEVRFST HLPQPHSICS LIVEPMESWL QLMLNWDPEQ
310 320 330 340 350
RGGGLDPETN SPKCFLLMDH ILNLKIVHIL NMTSAKIVSF LLHPEESLHF
360 370 380 390 400
LQNRIESETG ISTGNQELLL ETGICLDPRK PASQCVIDGV RGWDSYMVYL
410 420 430 440 450
FDKSKTVYDG PFASRSLSEC VNYIVQDSKI QLPIPQLRKV WAEAVHYVIG
460 470 480 490 500
LKEDYSRLFQ GQRAAMLSLL RYNANLIKMK NNMVSASQQL KAKLEFFHQS
510 520 530 540 550
IRLDLERYSD QMAYGISSEK MLKAWKEMEE KASHCAQAED IGYLDEQIMA
560 570 580 590 600
LHTEIVELQK SPYARRQGEV MESLEQRAID LYKQLKTRPP DHAYSDSTDM
610 620 630 640 650
VKIIVQTVQS QDRVLKELFG HLSKLLGCKQ KIIDLLPKIE VALNNIKEAD
660 670 680 690 700
NSEMQMQGKR QREIWHLLKI ACTQSSSRSL VSSSLEGTAS TPAATWVPQS
710 720 730 740 750
SSEYAPHPLS SMATPGDGEN FVDVIEENLN YLDRFSSMLQ EAREEQNNSL

TNFDWSWLK
Length:759
Mass (Da):86,144
Last modified:November 23, 2004 - v1
Checksum:i135FDF10396B7B96
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720520 mRNA. Translation: CAG32179.1.
RefSeqiNP_001012922.1. NM_001012904.1.
UniGeneiGga.5483.

Genome annotation databases

GeneIDi423669.
KEGGigga:423669.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720520 mRNA. Translation: CAG32179.1.
RefSeqiNP_001012922.1. NM_001012904.1.
UniGeneiGga.5483.

3D structure databases

ProteinModelPortaliQ5ZJB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000005196.

Proteomic databases

PaxDbiQ5ZJB4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi423669.
KEGGigga:423669.

Organism-specific databases

CTDi1147.

Phylogenomic databases

eggNOGiKOG4250. Eukaryota.
ENOG410XRMU. LUCA.
HOGENOMiHOG000038048.
HOVERGENiHBG018241.
InParanoidiQ5ZJB4.
KOiK04467.
PhylomeDBiQ5ZJB4.

Enzyme and pathway databases

ReactomeiR-GGA-1169091. Activation of NF-kappaB in B cells.
R-GGA-1227739. Caspase-8 and -10 mediated induction of NF-kB.
R-GGA-1227892. TRAF6 mediated NF-kB activation.
R-GGA-434001. TAK1 activates NFkB by phosphorylation and activation of IKKs complex.
R-GGA-434131. NFkB activation mediated by RIP1 complexed with activated TLR3.

Miscellaneous databases

PROiQ5ZJB4.

Family and domain databases

InterProiIPR022007. IKKbetaNEMObind.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF12179. IKKbetaNEMObind. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM01239. IKKbetaNEMObind. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIKKA_CHICK
AccessioniPrimary (citable) accession number: Q5ZJB4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: November 23, 2004
Last modified: October 5, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.