Q5ZJ66 (SYEM_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutamate--tRNA ligase, mitochondrial
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	EARS2
ORF Names:	RCJMB04_20f12

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 20	20	Mitochondrion Potential
	Chain	21 – 502	482	Probable glutamate--tRNA ligase, mitochondrial		PRO_0000254563
Regions
	Nucleotide binding	263 – 267	5	ATP By similarity
	Region	19 – 21	3	Glutamate binding By similarity
	Region	207 – 211	5	Glutamate binding By similarity
	Motif	24 – 32	9	"HIGH" region
	Motif	263 – 267	5	"KMSKS" region
Sites
	Binding site	29	1	ATP By similarity
	Binding site	55	1	Glutamate By similarity
	Binding site	225	1	Glutamate By similarity
	Binding site	228	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZJ66 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 1E84C3BB0E744570	FASTA	502	56,954
	10 20 30 40 50 60 MAGMLREVCG AAASGLRVRF GPSPTGFLHL GGLRTALYNY VFAKQQRGTF VLRVEDTDQG 70 80 90 100 110 120 RVVAGAAESI EDMLHWAGIP PDESPRRGGP FGPYQQSLRL DLYRAASEAL LDRGAAYRCF 130 140 150 160 170 180 CTPQRLELLR KEALRNQQTP RYDNRCRHLT PKEVAEKLAQ GLDWVVRFRL ERGVEPFQDL 190 200 210 220 230 240 VYGWNKHEVA EVEGDPVILK ADGFPTYHLA NVVDDHHMGI SHVLRGTEWL TSTSKHLLLY 250 260 270 280 290 300 KAFGWDPPQF GHLPLLLNKD GSKLSKRQGD IFLERFAQEG YLPEALLDMI TNCGSGFAEK 310 320 330 340 350 360 QMGRTLEELI SQFEIGRITT HSALLDLEKL PEFNRMHLTR HIENEGLRQK LIQELQLLVE 370 380 390 400 410 420 DVYGDQEVDK EVLEKEYVEQ VLLLRKGHIS HLKDLVSDNY SYLWVRPSVS REQLQMISAE 430 440 450 460 470 480 VDEIGKLVLG LMTKPAAVWT IEELNKDLRS LQKQTRETKY SSMMKLLRLA LSGQQHGPSV 490 500 AEMMVTLGPR EVCGRISKVL SS

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ720568 mRNA. Translation: CAG32227.1.
IPI	IPI00602811.
RefSeq	NP_001026638.1. NM_001031467.1.
UniGene	Gga.22559.
3D structure databases
HSSP	HSSP built from PDB template 1NZJ based on UniProtKB P27305.
ProteinModelPortal	Q5ZJ66.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000009866.
Proteomic databases
PaxDb	Q5ZJ66.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	427672.
KEGG	gga:427672.
Organism-specific databases
CTD	124454.
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000252720.
HOVERGEN	HBG056174.
InParanoid	Q5ZJ66.
KO	K01885.
OrthoDB	EOG418BN8.
Family and domain databases
Gene3D	1.10.10.350. 1 hit. 1.10.1160.10. 1 hit. 3.40.50.620. 2 hits.
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-ligase_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20828882.

Entry information

Entry name

SYEM_CHICK

Accession

Primary (citable) accession number: Q5ZJ66

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 23, 2004
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program