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Protein

Probable glutamate--tRNA ligase, mitochondrial

Gene

EARS2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29ATPBy similarity1
Binding sitei55GlutamateBy similarity1
Binding sitei225GlutamateBy similarity1
Binding sitei228ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi263 – 267ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:EARS2
ORF Names:RCJMB04_20f12
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 20MitochondrionSequence analysisAdd BLAST20
ChainiPRO_000025456321 – 502Probable glutamate--tRNA ligase, mitochondrialAdd BLAST482

Proteomic databases

PaxDbiQ5ZJ66.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000009866.

Structurei

3D structure databases

ProteinModelPortaliQ5ZJ66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 21Glutamate bindingBy similarity3
Regioni207 – 211Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi24 – 32"HIGH" region9
Motifi263 – 267"KMSKS" region5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1149. Eukaryota.
COG0008. LUCA.
HOGENOMiHOG000252720.
HOVERGENiHBG056174.
InParanoidiQ5ZJ66.
KOiK01885.
PhylomeDBiQ5ZJ66.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5ZJ66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGMLREVCG AAASGLRVRF GPSPTGFLHL GGLRTALYNY VFAKQQRGTF
60 70 80 90 100
VLRVEDTDQG RVVAGAAESI EDMLHWAGIP PDESPRRGGP FGPYQQSLRL
110 120 130 140 150
DLYRAASEAL LDRGAAYRCF CTPQRLELLR KEALRNQQTP RYDNRCRHLT
160 170 180 190 200
PKEVAEKLAQ GLDWVVRFRL ERGVEPFQDL VYGWNKHEVA EVEGDPVILK
210 220 230 240 250
ADGFPTYHLA NVVDDHHMGI SHVLRGTEWL TSTSKHLLLY KAFGWDPPQF
260 270 280 290 300
GHLPLLLNKD GSKLSKRQGD IFLERFAQEG YLPEALLDMI TNCGSGFAEK
310 320 330 340 350
QMGRTLEELI SQFEIGRITT HSALLDLEKL PEFNRMHLTR HIENEGLRQK
360 370 380 390 400
LIQELQLLVE DVYGDQEVDK EVLEKEYVEQ VLLLRKGHIS HLKDLVSDNY
410 420 430 440 450
SYLWVRPSVS REQLQMISAE VDEIGKLVLG LMTKPAAVWT IEELNKDLRS
460 470 480 490 500
LQKQTRETKY SSMMKLLRLA LSGQQHGPSV AEMMVTLGPR EVCGRISKVL

SS
Length:502
Mass (Da):56,954
Last modified:November 23, 2004 - v1
Checksum:i1E84C3BB0E744570
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720568 mRNA. Translation: CAG32227.1.
RefSeqiNP_001026638.1. NM_001031467.1.
UniGeneiGga.22559.

Genome annotation databases

GeneIDi427672.
KEGGigga:427672.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720568 mRNA. Translation: CAG32227.1.
RefSeqiNP_001026638.1. NM_001031467.1.
UniGeneiGga.22559.

3D structure databases

ProteinModelPortaliQ5ZJ66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000009866.

Proteomic databases

PaxDbiQ5ZJ66.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi427672.
KEGGigga:427672.

Organism-specific databases

CTDi124454.

Phylogenomic databases

eggNOGiKOG1149. Eukaryota.
COG0008. LUCA.
HOGENOMiHOG000252720.
HOVERGENiHBG056174.
InParanoidiQ5ZJ66.
KOiK01885.
PhylomeDBiQ5ZJ66.

Miscellaneous databases

PROiQ5ZJ66.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYEM_CHICK
AccessioniPrimary (citable) accession number: Q5ZJ66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 23, 2004
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.