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Q5ZJ66

- SYEM_CHICK

UniProt

Q5ZJ66 - SYEM_CHICK

Protein

Probable glutamate--tRNA ligase, mitochondrial

Gene

EARS2

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei29 – 291ATPBy similarity
    Binding sitei55 – 551GlutamateBy similarity
    Binding sitei225 – 2251GlutamateBy similarity
    Binding sitei228 – 2281ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi263 – 2675ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-tRNA ligase activity Source: UniProtKB-EC
    3. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glutamate--tRNA ligase, mitochondrial (EC:6.1.1.17)
    Alternative name(s):
    Glutamyl-tRNA synthetase
    Short name:
    GluRS
    Gene namesi
    Name:EARS2
    ORF Names:RCJMB04_20f12
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2020MitochondrionSequence AnalysisAdd
    BLAST
    Chaini21 – 502482Probable glutamate--tRNA ligase, mitochondrialPRO_0000254563Add
    BLAST

    Proteomic databases

    PaxDbiQ5ZJ66.

    Interactioni

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000009866.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5ZJ66.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni19 – 213Glutamate bindingBy similarity
    Regioni207 – 2115Glutamate bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi24 – 329"HIGH" region
    Motifi263 – 2675"KMSKS" region

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252720.
    HOVERGENiHBG056174.
    InParanoidiQ5ZJ66.
    KOiK01885.
    PhylomeDBiQ5ZJ66.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5ZJ66-1 [UniParc]FASTAAdd to Basket

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    MAGMLREVCG AAASGLRVRF GPSPTGFLHL GGLRTALYNY VFAKQQRGTF    50
    VLRVEDTDQG RVVAGAAESI EDMLHWAGIP PDESPRRGGP FGPYQQSLRL 100
    DLYRAASEAL LDRGAAYRCF CTPQRLELLR KEALRNQQTP RYDNRCRHLT 150
    PKEVAEKLAQ GLDWVVRFRL ERGVEPFQDL VYGWNKHEVA EVEGDPVILK 200
    ADGFPTYHLA NVVDDHHMGI SHVLRGTEWL TSTSKHLLLY KAFGWDPPQF 250
    GHLPLLLNKD GSKLSKRQGD IFLERFAQEG YLPEALLDMI TNCGSGFAEK 300
    QMGRTLEELI SQFEIGRITT HSALLDLEKL PEFNRMHLTR HIENEGLRQK 350
    LIQELQLLVE DVYGDQEVDK EVLEKEYVEQ VLLLRKGHIS HLKDLVSDNY 400
    SYLWVRPSVS REQLQMISAE VDEIGKLVLG LMTKPAAVWT IEELNKDLRS 450
    LQKQTRETKY SSMMKLLRLA LSGQQHGPSV AEMMVTLGPR EVCGRISKVL 500
    SS 502
    Length:502
    Mass (Da):56,954
    Last modified:November 23, 2004 - v1
    Checksum:i1E84C3BB0E744570
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720568 mRNA. Translation: CAG32227.1.
    RefSeqiNP_001026638.1. NM_001031467.1.
    UniGeneiGga.22559.

    Genome annotation databases

    GeneIDi427672.
    KEGGigga:427672.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720568 mRNA. Translation: CAG32227.1 .
    RefSeqi NP_001026638.1. NM_001031467.1.
    UniGenei Gga.22559.

    3D structure databases

    ProteinModelPortali Q5ZJ66.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000009866.

    Proteomic databases

    PaxDbi Q5ZJ66.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 427672.
    KEGGi gga:427672.

    Organism-specific databases

    CTDi 124454.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252720.
    HOVERGENi HBG056174.
    InParanoidi Q5ZJ66.
    KOi K01885.
    PhylomeDBi Q5ZJ66.

    Miscellaneous databases

    NextBioi 20828882.
    PROi Q5ZJ66.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: CB.
      Tissue: Bursa of Fabricius.

    Entry informationi

    Entry nameiSYEM_CHICK
    AccessioniPrimary (citable) accession number: Q5ZJ66
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2006
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3