Q5ZJ23 (ASSY_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate synthase
EC=6.3.4.5

Alternative name(s):
Citrulline--aspartate ligase

Gene names

Name:	ASS1
ORF Names:	RCJMB04_21j5

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. Nitrogen metabolism; urea cycle; (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline: step 1/1.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the argininosuccinate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Urea cycle
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway urea cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW argininosuccinate synthase activity Inferred from direct assay PubMed 14064979. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Argininosuccinate synthase		PRO_0000321322
Regions
	Nucleotide binding	11 – 19	9	ATP By similarity
	Nucleotide binding	116 – 124	9	ATP By similarity
Sites
	Binding site	37	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	88	1	Citrulline By similarity
	Binding site	120	1	Aspartate By similarity
	Binding site	124	1	Aspartate By similarity
	Binding site	124	1	Citrulline By similarity
	Binding site	125	1	Aspartate By similarity
	Binding site	128	1	Citrulline By similarity
	Binding site	181	1	Citrulline By similarity
	Binding site	190	1	Citrulline By similarity
	Binding site	271	1	Citrulline By similarity
	Binding site	283	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZJ23 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: AF0CC73CA67D1B71	FASTA	416	46,904
	10 20 30 40 50 60 MAEPRDTVVL AYSGGLDTSC ILVWLKEQGY TVIAFLANIG QTEDFDAAQK KALALGAKKV 70 80 90 100 110 120 YIQDVCREFV EDFIWPAVRA NALYEDRYML GSALARPCIA RHLVLIAQEE GARYIAHGAT 130 140 150 160 170 180 GKGNDQVRFE LGCYALCPSI KVIAPWRMPE FYQRFPGRRE LMEYAQKHGI PVPVTPKAPW 190 200 210 220 230 240 SMDENLMHIS YEAGILENPK NRAPLDLYTK TCNPTTSPDV PDELEIEFEK GVPVKVTNTR 250 260 270 280 290 300 NGVTHRSALE LFVYLNDIAS KHGVGRVDIV ENRFVGMKSR GIYETPAGTI LYHAHLDIEA 310 320 330 340 350 360 FTMDREVRKI KQGLSLKFSE LVYNGFWYSP ECEFLKHCIA RSQQAVAGTV HVSVFKGQVY 370 380 390 400 410 VLGRESPHSL YNEELVSMDV QGDYEPADAT GFININALRL KEYHRLQSKV STKQDE

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ720611 mRNA. Translation: CAG32270.1.
IPI	IPI00598406.
RefSeq	NP_001013413.1. NM_001013395.1.
UniGene	Gga.5385.
3D structure databases
HSSP	HSSP built from PDB template 1J20 based on UniProtKB P59846.
ProteinModelPortal	Q5ZJ23.
SMR	Q5ZJ23. Positions 5-408.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000006379.
Proteomic databases
PaxDb	Q5ZJ23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	417185.
KEGG	gga:417185.
Organism-specific databases
CTD	445.
Phylogenomic databases
eggNOG	COG0137.
HOGENOM	HOG000230093.
HOVERGEN	HBG001717.
InParanoid	Q5ZJ23.
KO	K01940.
OrthoDB	EOG45B1FK.
Enzyme and pathway databases
UniPathway	UPA00068; UER00113. UPA00158; UER00272.
Family and domain databases
Gene3D	3.40.50.620. 1 hit. 3.90.1260.10. 1 hit.
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR023434. Arginosuc_synth_type_1_subfam. IPR024074. AS_cat/multimer_dom_body. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR11587. PTHR11587. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20820530.

Entry information

Entry name

ASSY_CHICK

Accession

Primary (citable) accession number: Q5ZJ23

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program