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Protein

Argininosuccinate synthase

Gene

ASS1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.By similarity

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.By similarity

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.By similarity
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Argininosuccinate synthase (ASS1)
  3. Argininosuccinate lyase (ASL2)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Argininosuccinate synthase (ASS1)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (N(omega)-L-arginino)succinate from L-aspartate and L-citrulline, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37ATP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei88CitrullineBy similarity1
Binding sitei120AspartateBy similarity1
Binding sitei124AspartateBy similarity1
Binding sitei124CitrullineBy similarity1
Binding sitei125AspartateBy similarity1
Binding sitei128CitrullineBy similarity1
Binding sitei181CitrullineBy similarity1
Binding sitei190CitrullineBy similarity1
Binding sitei271CitrullineBy similarity1
Binding sitei283CitrullineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 19ATPBy similarity9
Nucleotide bindingi116 – 124ATPBy similarity9

GO - Molecular functioni

  • argininosuccinate synthase activity Source: AgBase
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Urea cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseCurated (EC:6.3.4.5By similarity)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:ASS1By similarity
ORF Names:RCJMB04_21j5Imported
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Cytoplasmcytosol By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003213221 – 416Argininosuccinate synthaseAdd BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei88PhosphotyrosineBy similarity1
Modified residuei114PhosphotyrosineBy similarity1
Modified residuei181PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5ZJ23.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006379.

Structurei

3D structure databases

ProteinModelPortaliQ5ZJ23.
SMRiQ5ZJ23.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family.Curated

Phylogenomic databases

eggNOGiKOG1706. Eukaryota.
COG0137. LUCA.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiQ5ZJ23.
KOiK01940.
PhylomeDBiQ5ZJ23.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR11587. PTHR11587. 1 hit.
PfamiView protein in Pfam
PF00764. Arginosuc_synth. 1 hit.
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiView protein in PROSITE
PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5ZJ23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPRDTVVL AYSGGLDTSC ILVWLKEQGY TVIAFLANIG QTEDFDAAQK
60 70 80 90 100
KALALGAKKV YIQDVCREFV EDFIWPAVRA NALYEDRYML GSALARPCIA
110 120 130 140 150
RHLVLIAQEE GARYIAHGAT GKGNDQVRFE LGCYALCPSI KVIAPWRMPE
160 170 180 190 200
FYQRFPGRRE LMEYAQKHGI PVPVTPKAPW SMDENLMHIS YEAGILENPK
210 220 230 240 250
NRAPLDLYTK TCNPTTSPDV PDELEIEFEK GVPVKVTNTR NGVTHRSALE
260 270 280 290 300
LFVYLNDIAS KHGVGRVDIV ENRFVGMKSR GIYETPAGTI LYHAHLDIEA
310 320 330 340 350
FTMDREVRKI KQGLSLKFSE LVYNGFWYSP ECEFLKHCIA RSQQAVAGTV
360 370 380 390 400
HVSVFKGQVY VLGRESPHSL YNEELVSMDV QGDYEPADAT GFININALRL
410
KEYHRLQSKV STKQDE
Length:416
Mass (Da):46,904
Last modified:November 23, 2004 - v1
Checksum:iAF0CC73CA67D1B71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720611 mRNA. Translation: CAG32270.1.
RefSeqiNP_001013413.1. NM_001013395.1.
UniGeneiGga.5385.

Genome annotation databases

GeneIDi417185.
KEGGigga:417185.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720611 mRNA. Translation: CAG32270.1.
RefSeqiNP_001013413.1. NM_001013395.1.
UniGeneiGga.5385.

3D structure databases

ProteinModelPortaliQ5ZJ23.
SMRiQ5ZJ23.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006379.

Proteomic databases

PaxDbiQ5ZJ23.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi417185.
KEGGigga:417185.

Organism-specific databases

CTDi445.

Phylogenomic databases

eggNOGiKOG1706. Eukaryota.
COG0137. LUCA.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiQ5ZJ23.
KOiK01940.
PhylomeDBiQ5ZJ23.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Miscellaneous databases

PROiPR:Q5ZJ23.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
PANTHERiPTHR11587. PTHR11587. 1 hit.
PfamiView protein in Pfam
PF00764. Arginosuc_synth. 1 hit.
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiView protein in PROSITE
PS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_CHICK
AccessioniPrimary (citable) accession number: Q5ZJ23
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 23, 2004
Last modified: May 10, 2017
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.