Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Argininosuccinate synthase

Gene

ASS1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei88 – 881CitrullineBy similarity
Binding sitei120 – 1201AspartateBy similarity
Binding sitei124 – 1241AspartateBy similarity
Binding sitei124 – 1241CitrullineBy similarity
Binding sitei125 – 1251AspartateBy similarity
Binding sitei128 – 1281CitrullineBy similarity
Binding sitei181 – 1811CitrullineBy similarity
Binding sitei190 – 1901CitrullineBy similarity
Binding sitei271 – 2711CitrullineBy similarity
Binding sitei283 – 2831CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 199ATPBy similarity
Nucleotide bindingi116 – 1249ATPBy similarity

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: AgBase
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-UniPathway
  2. urea cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:ASS1
ORF Names:RCJMB04_21j5
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Argininosuccinate synthasePRO_0000321322Add
BLAST

Proteomic databases

PaxDbiQ5ZJ23.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006379.

Structurei

3D structure databases

ProteinModelPortaliQ5ZJ23.
SMRiQ5ZJ23. Positions 5-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiQ5ZJ23.
KOiK01940.
PhylomeDBiQ5ZJ23.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZJ23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPRDTVVL AYSGGLDTSC ILVWLKEQGY TVIAFLANIG QTEDFDAAQK
60 70 80 90 100
KALALGAKKV YIQDVCREFV EDFIWPAVRA NALYEDRYML GSALARPCIA
110 120 130 140 150
RHLVLIAQEE GARYIAHGAT GKGNDQVRFE LGCYALCPSI KVIAPWRMPE
160 170 180 190 200
FYQRFPGRRE LMEYAQKHGI PVPVTPKAPW SMDENLMHIS YEAGILENPK
210 220 230 240 250
NRAPLDLYTK TCNPTTSPDV PDELEIEFEK GVPVKVTNTR NGVTHRSALE
260 270 280 290 300
LFVYLNDIAS KHGVGRVDIV ENRFVGMKSR GIYETPAGTI LYHAHLDIEA
310 320 330 340 350
FTMDREVRKI KQGLSLKFSE LVYNGFWYSP ECEFLKHCIA RSQQAVAGTV
360 370 380 390 400
HVSVFKGQVY VLGRESPHSL YNEELVSMDV QGDYEPADAT GFININALRL
410
KEYHRLQSKV STKQDE
Length:416
Mass (Da):46,904
Last modified:November 23, 2004 - v1
Checksum:iAF0CC73CA67D1B71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720611 mRNA. Translation: CAG32270.1.
RefSeqiNP_001013413.1. NM_001013395.1.
UniGeneiGga.5385.

Genome annotation databases

GeneIDi417185.
KEGGigga:417185.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720611 mRNA. Translation: CAG32270.1.
RefSeqiNP_001013413.1. NM_001013395.1.
UniGeneiGga.5385.

3D structure databases

ProteinModelPortaliQ5ZJ23.
SMRiQ5ZJ23. Positions 5-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000006379.

Proteomic databases

PaxDbiQ5ZJ23.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi417185.
KEGGigga:417185.

Organism-specific databases

CTDi445.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
HOVERGENiHBG001717.
InParanoidiQ5ZJ23.
KOiK01940.
PhylomeDBiQ5ZJ23.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
UPA00158; UER00272.

Miscellaneous databases

NextBioi20820530.
PROiQ5ZJ23.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiASSY_CHICK
AccessioniPrimary (citable) accession number: Q5ZJ23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: November 23, 2004
Last modified: February 4, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.