Q5ZJ08 (SYYC_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase, cytoplasmic
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:	YARS
ORF Names:	RCJMB04_21p3

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Tyrosine--tRNA ligase, cytoplasmic		PRO_0000239692
Regions
	Domain	363 – 467	105	tRNA-binding
	Motif	44 – 52	9	"HIGH" region By similarity
	Motif	222 – 226	5	"KMSKS" region By similarity
Sites
	Binding site	39	1	Tyrosine By similarity
	Binding site	166	1	Tyrosine By similarity
	Binding site	170	1	Tyrosine By similarity
	Binding site	173	1	Tyrosine By similarity
	Binding site	188	1	Tyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZJ08 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: C09BB7DAB8B89D60	FASTA	527	59,284
	10 20 30 40 50 60 METASGPQEK YQLITRNLQE VLGEDKLMAI LKEREVKIYW GTATTGKPHV AYFVPMSKIA 70 80 90 100 110 120 DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRTRYYEHV IKAMLESIGV PLEKLKFIRG 130 140 150 160 170 180 TDYQLSKEYT LDVYRLSSVV TQHDAKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD 190 200 210 220 230 240 AQFGGVDQRK IFTFAEKYLP SLGYAKRIHL MNPMVPGLTG SKMSSSEEDS KIDLLDRKED 250 260 270 280 290 300 VKKKLKKAFC EPGNIENNGV LSFIKHVLFP LKSEFVILRE EKWGGNKTYT AYETLEKDFA 310 320 330 340 350 360 EQVVHPGDLK NSVEAALNKL LDPIREKFNS PELKKLTNAA YPNPSKAKPA EKGTKNSEPE 370 380 390 400 410 420 TIVPSRLDIR VGKVVSVEKH PDADSLYVEK IDVGEPEPRT VVSGLVQFVP KEQLQDRLVV 430 440 450 460 470 480 LLCNLKPQKM RGVESQGMVL CASSVGEPRQ VEPLDPPAEC CAGERVYVEG YEDGEPDDEL 490 500 510 520 KPKKKVFEKL QADFRISEDC IAQWKERNFL TKLGSISCKS LKGGSIS

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ720626 mRNA. Translation: CAG32285.1.
IPI	IPI00583766.
RefSeq	NP_001006314.1. NM_001006314.1.
UniGene	Gga.4637.
3D structure databases
ProteinModelPortal	Q5ZJ08.
SMR	Q5ZJ08. Positions 4-342, 359-527.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZJ08.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	419666.
KEGG	gga:419666.
Organism-specific databases
CTD	8565.
Phylogenomic databases
eggNOG	veNOG07402.
GeneTree	ENSGT00550000074927.
HOGENOM	HBG330667.
HOVERGEN	HBG080113.
InParanoid	Q5ZJ08.
OrthoDB	EOG4RV2R7.
PhylomeDB	Q5ZJ08.
Family and domain databases
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR002307. Tyr-tRNA-synth. IPR023617. Tyr-tRNA-synth_arc-type. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11946:SF8. Tyr-tRNA_synth. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYYC_CHICK

Accession

Primary (citable) accession number: Q5ZJ08

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program