Reviewed, UniProtKB/Swiss-Prot Q5ZIZ4 (5NTC_CHICK)

Names and origin

Protein names

Recommended name:
    Cytosolic purine 5'-nucleotidase
    EC=3.1.3.5
Alternative name(s):
    5'-nucleotidase cytosolic II

Gene names

Name:	NT5C2
ORF Names:	RCJMB04_22h21

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides By similarity.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Enzyme regulation	Allosterically activated by various compounds, including ATP By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the 5'(3')-deoxyribonucleotidase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Cytosolic purine 5'-nucleotidase		PRO_0000310266
Regions
	Region	202 – 210	9	Substrate binding Potential
	Compositional bias	549 – 569	21	Asp/Glu-rich (acidic)
Sites
	Active site	52	1	Nucleophile By similarity
	Active site	54	1	Proton donor By similarity
	Metal binding	52	1	Magnesium By similarity
	Metal binding	54	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	351	1	Magnesium By similarity
	Binding site	127	1	Allosteric activator 1 By similarity
	Binding site	154	1	Allosteric activator 2 By similarity
	Binding site	354	1	Allosteric activator 2 By similarity
	Binding site	436	1	Allosteric activator 1; via carbonyl oxygen By similarity
	Binding site	453	1	Allosteric activator 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZIZ4-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: C8FD122625879CB6	FASTA	569	65,894
	10 20 30 40 50 60 MTTSWSDRLQ NAADLPANMD GHALKKYRRE AYHRVFVNRS LAMEKIKCFG FDMDYTLAVY 70 80 90 100 110 120 KSPEYESLGF DLTVERLVSI GYPHELLNFV YDPAFPTRGL VFDTHYGNLL KVDAYGNLLV 130 140 150 160 170 180 CAHGFNFLRG PETRDQYPNK FIQRDDTDRF YILNTLFNLP ETYLLACLVD FFTNCDRYTS 190 200 210 220 230 240 CETGFKDGDL FMSFRSMFQD VRDAVDWVHY KGSLKEKTLE NLEKYVVKDG KLPLLLSRMN 250 260 270 280 290 300 EVGKVFLVTN SDYKYTDKIM TYLFDFPHGP KPGSAHRPWQ SYFDLILVDA RKPLFFGEGT 310 320 330 340 350 360 VLRQVDTVTG KLKIGTYTGP LQHGIVYSGG SSDTVCDLLG AKGKDILYIG DHIFGDILKS 370 380 390 400 410 420 KKRQGWRTFL VIPELAQELH VWTDKSALFE ELQSLDIFLA ELYKHLDSSS NERPDISSIQ 430 440 450 460 470 480 RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF ASQVMRYADL YAASFINLLY YPFSYLFRAA 490 500 510 520 530 540 HVLMPHESTV EHTHVDINEK ESPMATRNRT SVDFKDSDYK RHQLTRSISE IKPPNLFPQA 550 560 PQEITHCHDE DDDEEEEEEE VEEEEEEEE

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ720640 mRNA. Translation: CAG32299.1.
IPI	IPI00583285.
RefSeq	NP_001026405.1.
UniGene	Gga.22448
3D structure databases
SMR	Q5ZIZ4. Positions 3-488.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZIZ4.
Genome annotation databases
Ensembl	ENSGALT00000013309; ENSGALP00000013294; ENSGALG00000008179; Gallus gallus. [Genome view]
GeneID	423871.
KEGG	gga:423871.
Organism-specific databases
CTD	423871.
Phylogenomic databases
HOGENOM	Q5ZIZ4.
HOVERGEN	Q5ZIZ4.
Enzyme and pathway databases
BRENDA	3.1.3.5. 4.
Family and domain databases
InterPro	IPR008380. HAD-SF_hydro_IG_5-nucl. IPR016695. Pur_nucleotidase. [Graphical view]
PANTHER	PTHR12103. HAD-SF_hydro_IG_5-nucl. 1 hit.
Pfam	PF05761. 5_nucleotid. 1 hit. [Graphical view]
PIRSF	PIRSF017434. Purine_5'-nucleotidase. 1 hit.
TIGRFAMs	TIGR02244. HAD-IG-Ncltidse. 1 hit.
ProtoNet	Search...

Entry information

Entry name

5NTC_CHICK

Accession

Primary (citable) accession number: Q5ZIZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	November 23, 2004
Last modified:	September 1, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)