Q5ZIU3 (DYRK2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity tyrosine-phosphorylation-regulated kinase 2
EC=2.7.12.1

Gene names

Name:	DYRK2
ORF Names:	RCJMB04_23i19

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Serine/threonine-protein kinase involved in the control of mitotic transition and the regulation of cellular growth and/or development By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q92630
Cofactor	Magnesium By similarity. UniProtKB Q92630 Manganese By similarity. UniProtKB Q92630
Enzyme regulation	Autophosphorylates on tyrosine residues By similarity. UniProtKB Q92630
Subunit structure	Interacts with MDM2 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity UniProtKB Q92630.
Post-translational modification	Phosphorylated on serine/threonine residues. Phosphorylation on Thr-31 and Ser-367 by ATM in response to genotoxic stress disrupts MDM2 binding and prevents MDM2-mediated ubiquitination and subsequent proteasome degradation, thus promoting p53/TP53-mediated apoptosis By similarity. Ubiquitination in nucleus by MDM2 in normal conditions leads to proteasome degradation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Magnesium Manganese Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis Inferred from sequence or structural similarity. Source: UniProtKB apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of glycogen biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from sequence or structural similarity. Source: UniProtKB manganese ion binding Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine/tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	Dual specificity tyrosine-phosphorylation-regulated kinase 2		PRO_0000291266
Regions
	Domain	147 – 460	314	Protein kinase
	Nucleotide binding	153 – 161	9	ATP By similarity UniProtKB P28523
	Motif	114 – 116	3	Nuclear localization signal By similarity
Sites
	Active site	273	1	Proton acceptor By similarity UniProtKB P28523
	Binding site	176	1	ATP By similarity UniProtKB P28523
Amino acid modifications
	Modified residue	31	1	Phosphothreonine; by ATM By similarity
	Modified residue	307	1	Phosphotyrosine By similarity
	Modified residue	367	1	Phosphoserine; by ATM By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZIU3 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: E294B808874225A2	FASTA	526	59,437
	10 20 30 40 50 60 MNEHLHVGSH GQIQVQQLFE DNSNKRTVLT TQPNGLTTLG KSGLPVVQDR QSESAHRRQG 70 80 90 100 110 120 SSSSLKSTDG TGKVKASVMT PEQAMKQYMQ KLTTFEHNEI FGYTEIYFLG PNAKKRQGVI 130 140 150 160 170 180 GGPNNCGYDD DQGSYIQVPH DHIAYRYEVL KVIGKGSFGQ VVKAYDHKMH QHVALKMVRN 190 200 210 220 230 240 EKRFHRQAAE EIRILEHLRK QDKDNNMNVI HMLENFTFRS HICMTFELLS MNLYELIKKN 250 260 270 280 290 300 KFQGFSLPLV RKFAHSILQC LDALHKNRII HCDLKPENIL LKQQGRSGIK VIDFGSSCYE 310 320 330 340 350 360 HQRVYTYIQS RFYRAPEVIL GARYGMPIDM WSLGCILAEL LTGYPLLPGE DEGDQLACMI 370 380 390 400 410 420 ELLGMPSPKL LDSSKRAKNF VSSKGYPRYC TITTLSDGSI ILNGGRSRRG KLRGPPESRE 430 440 450 460 470 480 WGNALKGCDD PLFLDFLKQC LEWDPAIRMT PSQALRHPWL RRRLPKPPTG EKASAKRITE 490 500 510 520 STGAITSISK LPPTSSSASK LRTNLAQMTD ANGNIQQRTV LPKLVS

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ720691 mRNA. Translation: CAG32350.1.
IPI	IPI00590172.
RefSeq	NP_001026651.1. NM_001031480.1.
UniGene	Gga.14403.
3D structure databases
HSSP	HSSP built from PDB template 1UKH based on UniProtKB P45983.
ProteinModelPortal	Q5ZIU3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000016103; ENSGALP00000016084; ENSGALG00000009901.
GeneID	427864.
KEGG	gga:427864.
Organism-specific databases
CTD	8445.
Phylogenomic databases
eggNOG	veNOG08694.
GeneTree	ENSGT00570000079111.
HOGENOM	HBG755340.
HOVERGEN	HBG051426.
InParanoid	Q5ZIU3.
OMA	QVPHDHV.
OrthoDB	EOG4QRH3Z.
PhylomeDB	Q5ZIU3.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08825.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYRK2_CHICK

Accession

Primary (citable) accession number: Q5ZIU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program