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Q5ZIN2 (SEN2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease subunit Sen2

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease Sen2
Gene names
Name:TSEN2
ORF Names:RCJMB04_24m3
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length461 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events By similarity.

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Subunit structure

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the tRNA-intron endonuclease family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 461461tRNA-splicing endonuclease subunit Sen2
PRO_0000109455

Sites

Active site3651 By similarity
Active site3731 By similarity
Active site4121 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5ZIN2 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 69108F9DAE6EC982

FASTA46152,134
        10         20         30         40         50         60 
MAEAAFHPPR RKRRVFECYQ APFPVGPGAG DFRMCRADMV NNCVVVRSPE DMDQLYNKGY 

        70         80         90        100        110        120 
FGKGILSRSR PVYSISDPSL VSKWQGANLN VPIITSKKYQ QRVQWAKSAL QEQGFDGCSV 

       130        140        150        160        170        180 
TKILENYTRP LEFPFLEKGG AEQTGDSCDT VCPNTENTEL SGQSSTDTGN IATSSPECQK 

       190        200        210        220        230        240 
EVCQKAYAEG DPASDSMVGS KEQEPADVKE TAQTSCQMHG SCMHCGCKAK GAPDRESCNM 

       250        260        270        280        290        300 
AKSRERAPEY VLVQEEEESS LCPEEGSARE QENFVKKEKL VCRRNPFRIF EYLQLSLEEA 

       310        320        330        340        350        360 
FFLVYALGCL TVYYGEEPLT ILKLWEIFSE VKPSFKTTYM AYHYFRSKGW VPKVGLKYGT 

       370        380        390        400        410        420 
DLLLYRKGPP FYHASYSVIA ELVDDNFEGS LRRPLSWMSL SGLNRTTVNA SKELLLCYLI 

       430        440        450        460 
KPSDMTEEEM ATPECLKRIK VQELIVTRWV SSRERSEQDD L 

« Hide

References

[1]"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis."
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.
Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: CB.
Tissue: Bursa of Fabricius.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ720752 mRNA. Translation: CAG32411.1.
RefSeqNP_001025765.1. NM_001030594.2.
UniGeneGga.22337.

3D structure databases

ProteinModelPortalQ5ZIN2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000007969.

Proteomic databases

PaxDbQ5ZIN2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID415965.
KEGGgga:415965.

Organism-specific databases

CTD80746.

Phylogenomic databases

eggNOGCOG1676.
HOGENOMHOG000154285.
HOVERGENHBG056610.
InParanoidQ5ZIN2.
KOK15322.
PhylomeDBQ5ZIN2.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR016589. tRNA_splic_SEN2.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFPIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMSSF53032. SSF53032. 1 hit.
ProtoNetSearch...

Other

NextBio20819488.
PROQ5ZIN2.

Entry information

Entry nameSEN2_CHICK
AccessionPrimary (citable) accession number: Q5ZIN2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families