Q5ZIN2 (SEN2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease subunit Sen2
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease Sen2

Gene names

Name:	TSEN2
ORF Names:	RCJMB04_24m3

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events By similarity.
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.
Subunit structure	tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family.

Ontologies

Keywords
Biological process	mRNA processing tRNA processing
Cellular component	Nucleus
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: InterPro
Cellular_component	tRNA-intron endonuclease complex Inferred from electronic annotation. Source: InterPro
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	tRNA-splicing endonuclease subunit Sen2		PRO_0000109455
Sites
	Active site	365	1	By similarity
	Active site	373	1	By similarity
	Active site	412	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZIN2 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 69108F9DAE6EC982	FASTA	461	52,134
	10 20 30 40 50 60 MAEAAFHPPR RKRRVFECYQ APFPVGPGAG DFRMCRADMV NNCVVVRSPE DMDQLYNKGY 70 80 90 100 110 120 FGKGILSRSR PVYSISDPSL VSKWQGANLN VPIITSKKYQ QRVQWAKSAL QEQGFDGCSV 130 140 150 160 170 180 TKILENYTRP LEFPFLEKGG AEQTGDSCDT VCPNTENTEL SGQSSTDTGN IATSSPECQK 190 200 210 220 230 240 EVCQKAYAEG DPASDSMVGS KEQEPADVKE TAQTSCQMHG SCMHCGCKAK GAPDRESCNM 250 260 270 280 290 300 AKSRERAPEY VLVQEEEESS LCPEEGSARE QENFVKKEKL VCRRNPFRIF EYLQLSLEEA 310 320 330 340 350 360 FFLVYALGCL TVYYGEEPLT ILKLWEIFSE VKPSFKTTYM AYHYFRSKGW VPKVGLKYGT 370 380 390 400 410 420 DLLLYRKGPP FYHASYSVIA ELVDDNFEGS LRRPLSWMSL SGLNRTTVNA SKELLLCYLI 430 440 450 460 KPSDMTEEEM ATPECLKRIK VQELIVTRWV SSRERSEQDD L

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ720752 mRNA. Translation: CAG32411.1.
IPI	IPI00590054.
RefSeq	NP_001025765.1. NM_001030594.2.
UniGene	Gga.22337.
3D structure databases
ProteinModelPortal	Q5ZIN2.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000007969.
Proteomic databases
PaxDb	Q5ZIN2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	415965.
KEGG	gga:415965.
Organism-specific databases
CTD	80746.
Phylogenomic databases
eggNOG	COG1676.
HOGENOM	HOG000154285.
HOVERGEN	HBG056610.
InParanoid	Q5ZIN2.
KO	K15322.
OrthoDB	EOG4Z36DM.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. IPR016589. tRNA_splic_SEN2. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. PF02778. tRNA_int_endo_N. 1 hit. [Graphical view]
PIRSF	PIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit.
ProtoNet	Search...
Other
NextBio	20819488.

Entry information

Entry name

SEN2_CHICK

Accession

Primary (citable) accession number: Q5ZIN2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program