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Q5ZIN2

- SEN2_CHICK

UniProt

Q5ZIN2 - SEN2_CHICK

Protein

tRNA-splicing endonuclease subunit Sen2

Gene

TSEN2

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei365 – 3651By similarity
    Active sitei373 – 3731By similarity
    Active sitei412 – 4121By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: InterPro

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    mRNA processing, tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonuclease subunit Sen2 (EC:4.6.1.16)
    Alternative name(s):
    tRNA-intron endonuclease Sen2
    Gene namesi
    Name:TSEN2
    ORF Names:RCJMB04_24m3
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. tRNA-intron endonuclease complex Source: InterPro

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 461461tRNA-splicing endonuclease subunit Sen2PRO_0000109455Add
    BLAST

    Proteomic databases

    PaxDbiQ5ZIN2.

    Interactioni

    Subunit structurei

    tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54.By similarity

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000007969.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5ZIN2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiCOG1676.
    HOGENOMiHOG000154285.
    HOVERGENiHBG056610.
    InParanoidiQ5ZIN2.
    KOiK15322.
    PhylomeDBiQ5ZIN2.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR016589. tRNA_splic_SEN2.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF011789. tRNA_splic_SEN2. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5ZIN2-1 [UniParc]FASTAAdd to Basket

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    MAEAAFHPPR RKRRVFECYQ APFPVGPGAG DFRMCRADMV NNCVVVRSPE    50
    DMDQLYNKGY FGKGILSRSR PVYSISDPSL VSKWQGANLN VPIITSKKYQ 100
    QRVQWAKSAL QEQGFDGCSV TKILENYTRP LEFPFLEKGG AEQTGDSCDT 150
    VCPNTENTEL SGQSSTDTGN IATSSPECQK EVCQKAYAEG DPASDSMVGS 200
    KEQEPADVKE TAQTSCQMHG SCMHCGCKAK GAPDRESCNM AKSRERAPEY 250
    VLVQEEEESS LCPEEGSARE QENFVKKEKL VCRRNPFRIF EYLQLSLEEA 300
    FFLVYALGCL TVYYGEEPLT ILKLWEIFSE VKPSFKTTYM AYHYFRSKGW 350
    VPKVGLKYGT DLLLYRKGPP FYHASYSVIA ELVDDNFEGS LRRPLSWMSL 400
    SGLNRTTVNA SKELLLCYLI KPSDMTEEEM ATPECLKRIK VQELIVTRWV 450
    SSRERSEQDD L 461
    Length:461
    Mass (Da):52,134
    Last modified:November 23, 2004 - v1
    Checksum:i69108F9DAE6EC982
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720752 mRNA. Translation: CAG32411.1.
    RefSeqiNP_001025765.1. NM_001030594.2.
    UniGeneiGga.22337.

    Genome annotation databases

    GeneIDi415965.
    KEGGigga:415965.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720752 mRNA. Translation: CAG32411.1 .
    RefSeqi NP_001025765.1. NM_001030594.2.
    UniGenei Gga.22337.

    3D structure databases

    ProteinModelPortali Q5ZIN2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000007969.

    Proteomic databases

    PaxDbi Q5ZIN2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 415965.
    KEGGi gga:415965.

    Organism-specific databases

    CTDi 80746.

    Phylogenomic databases

    eggNOGi COG1676.
    HOGENOMi HOG000154285.
    HOVERGENi HBG056610.
    InParanoidi Q5ZIN2.
    KOi K15322.
    PhylomeDBi Q5ZIN2.

    Miscellaneous databases

    NextBioi 20819488.
    PROi Q5ZIN2.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR016589. tRNA_splic_SEN2.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    PF02778. tRNA_int_endo_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF011789. tRNA_splic_SEN2. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: CB.
      Tissue: Bursa of Fabricius.

    Entry informationi

    Entry nameiSEN2_CHICK
    AccessioniPrimary (citable) accession number: Q5ZIN2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3