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Protein

tRNA-splicing endonuclease subunit Sen2

Gene

TSEN2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei365By similarity1
Active sitei373By similarity1
Active sitei412By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processmRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease Sen2
Gene namesi
Name:TSEN2
ORF Names:RCJMB04_24m3
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094551 – 461tRNA-splicing endonuclease subunit Sen2Add BLAST461

Proteomic databases

PaxDbiQ5ZIN2

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000007969

Structurei

3D structure databases

ProteinModelPortaliQ5ZIN2
SMRiQ5ZIN2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

eggNOGiKOG4685 Eukaryota
COG1676 LUCA
HOGENOMiHOG000154285
HOVERGENiHBG056610
InParanoidiQ5ZIN2
KOiK15322
PhylomeDBiQ5ZIN2

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR006676 tRNA_splic
IPR016589 tRNA_splic_SEN2
PANTHERiPTHR21227 PTHR21227, 1 hit
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 1 hit
PIRSFiPIRSF011789 tRNA_splic_SEN2, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit

Sequencei

Sequence statusi: Complete.

Q5ZIN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAAFHPPR RKRRVFECYQ APFPVGPGAG DFRMCRADMV NNCVVVRSPE
60 70 80 90 100
DMDQLYNKGY FGKGILSRSR PVYSISDPSL VSKWQGANLN VPIITSKKYQ
110 120 130 140 150
QRVQWAKSAL QEQGFDGCSV TKILENYTRP LEFPFLEKGG AEQTGDSCDT
160 170 180 190 200
VCPNTENTEL SGQSSTDTGN IATSSPECQK EVCQKAYAEG DPASDSMVGS
210 220 230 240 250
KEQEPADVKE TAQTSCQMHG SCMHCGCKAK GAPDRESCNM AKSRERAPEY
260 270 280 290 300
VLVQEEEESS LCPEEGSARE QENFVKKEKL VCRRNPFRIF EYLQLSLEEA
310 320 330 340 350
FFLVYALGCL TVYYGEEPLT ILKLWEIFSE VKPSFKTTYM AYHYFRSKGW
360 370 380 390 400
VPKVGLKYGT DLLLYRKGPP FYHASYSVIA ELVDDNFEGS LRRPLSWMSL
410 420 430 440 450
SGLNRTTVNA SKELLLCYLI KPSDMTEEEM ATPECLKRIK VQELIVTRWV
460
SSRERSEQDD L
Length:461
Mass (Da):52,134
Last modified:November 23, 2004 - v1
Checksum:i69108F9DAE6EC982
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720752 mRNA Translation: CAG32411.1
RefSeqiNP_001025765.1, NM_001030594.2
UniGeneiGga.22337

Genome annotation databases

GeneIDi415965
KEGGigga:415965

Similar proteinsi

Entry informationi

Entry nameiSEN2_CHICK
AccessioniPrimary (citable) accession number: Q5ZIN2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 23, 2004
Last modified: March 28, 2018
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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