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Q5ZIN2

- SEN2_CHICK

UniProt

Q5ZIN2 - SEN2_CHICK

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Protein
tRNA-splicing endonuclease subunit Sen2
Gene
TSEN2, RCJMB04_24m3
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events By similarity.

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei365 – 3651 By similarity
Active sitei373 – 3731 By similarity
Active sitei412 – 4121 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: InterPro

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. tRNA splicing, via endonucleolytic cleavage and ligation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

mRNA processing, tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit Sen2 (EC:4.6.1.16)
Alternative name(s):
tRNA-intron endonuclease Sen2
Gene namesi
Name:TSEN2
ORF Names:RCJMB04_24m3
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. tRNA-intron endonuclease complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461tRNA-splicing endonuclease subunit Sen2
PRO_0000109455Add
BLAST

Proteomic databases

PaxDbiQ5ZIN2.

Interactioni

Subunit structurei

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54 By similarity.

Protein-protein interaction databases

STRINGi9031.ENSGALP00000007969.

Structurei

3D structure databases

ProteinModelPortaliQ5ZIN2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
HOGENOMiHOG000154285.
HOVERGENiHBG056610.
InParanoidiQ5ZIN2.
KOiK15322.
PhylomeDBiQ5ZIN2.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR016589. tRNA_splic_SEN2.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
PIRSFiPIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5ZIN2-1 [UniParc]FASTAAdd to Basket

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MAEAAFHPPR RKRRVFECYQ APFPVGPGAG DFRMCRADMV NNCVVVRSPE    50
DMDQLYNKGY FGKGILSRSR PVYSISDPSL VSKWQGANLN VPIITSKKYQ 100
QRVQWAKSAL QEQGFDGCSV TKILENYTRP LEFPFLEKGG AEQTGDSCDT 150
VCPNTENTEL SGQSSTDTGN IATSSPECQK EVCQKAYAEG DPASDSMVGS 200
KEQEPADVKE TAQTSCQMHG SCMHCGCKAK GAPDRESCNM AKSRERAPEY 250
VLVQEEEESS LCPEEGSARE QENFVKKEKL VCRRNPFRIF EYLQLSLEEA 300
FFLVYALGCL TVYYGEEPLT ILKLWEIFSE VKPSFKTTYM AYHYFRSKGW 350
VPKVGLKYGT DLLLYRKGPP FYHASYSVIA ELVDDNFEGS LRRPLSWMSL 400
SGLNRTTVNA SKELLLCYLI KPSDMTEEEM ATPECLKRIK VQELIVTRWV 450
SSRERSEQDD L 461
Length:461
Mass (Da):52,134
Last modified:November 23, 2004 - v1
Checksum:i69108F9DAE6EC982
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ720752 mRNA. Translation: CAG32411.1.
RefSeqiNP_001025765.1. NM_001030594.2.
UniGeneiGga.22337.

Genome annotation databases

GeneIDi415965.
KEGGigga:415965.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ720752 mRNA. Translation: CAG32411.1 .
RefSeqi NP_001025765.1. NM_001030594.2.
UniGenei Gga.22337.

3D structure databases

ProteinModelPortali Q5ZIN2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000007969.

Proteomic databases

PaxDbi Q5ZIN2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 415965.
KEGGi gga:415965.

Organism-specific databases

CTDi 80746.

Phylogenomic databases

eggNOGi COG1676.
HOGENOMi HOG000154285.
HOVERGENi HBG056610.
InParanoidi Q5ZIN2.
KOi K15322.
PhylomeDBi Q5ZIN2.

Miscellaneous databases

NextBioi 20819488.
PROi Q5ZIN2.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR016589. tRNA_splic_SEN2.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF011789. tRNA_splic_SEN2. 1 hit.
SUPFAMi SSF53032. SSF53032. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiSEN2_CHICK
AccessioniPrimary (citable) accession number: Q5ZIN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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