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Protein

Cytosolic 5'-nucleotidase 3A

Gene

NT5C3A

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m7GMP). CMP seems to be the preferred substrate.By similarity

Catalytic activityi

N7-methyl-GMP + H2O = N7-methyl-guanosine + phosphate.By similarity
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei83NucleophileBy similarity1
Metal bindingi83MagnesiumBy similarity1
Active sitei85Proton donorBy similarity1
Metal bindingi85Magnesium; via carbonyl oxygenBy similarity1
Binding sitei130CMPBy similarity1
Binding sitei130N(7)-methyl-GMPBy similarity1
Binding sitei151N(7)-methyl-GMPBy similarity1
Binding sitei247SubstrateBy similarity1
Metal bindingi272MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transferase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-73621. Pyrimidine catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic 5'-nucleotidase 3ABy similarity (EC:3.1.3.5By similarity)
Alternative name(s):
7-methylguanosine phosphate-specific 5'-nucleotidaseBy similarity (EC:3.1.3.91By similarity)
Short name:
7-methylguanosine nucleotidase
Cytosolic 5'-nucleotidase 3
Cytosolic 5'-nucleotidase III
Short name:
cN-III
Pyrimidine 5'-nucleotidase 1
Short name:
P5'N-1
Short name:
P5N-1
Short name:
PN-I
Gene namesi
Name:NT5C3A
Synonyms:NT5C3, PYN1
ORF Names:RCJMB04_27l2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000643861 – 331Cytosolic 5'-nucleotidase 3AAdd BLAST331

Proteomic databases

PaxDbiQ5ZID6.

Expressioni

Developmental stagei

Expressed at low levels until E13 and the expression rises transiently between E13 and E16.1 Publication

Inductioni

By norepinephrine/NE and adenosine which are released by the embryo under hypoxic conditions. By stimulation of beta-adrenergic/adenosine receptors in definitive red blood cells (RBC).1 Publication

Gene expression databases

BgeeiENSGALG00000012192.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000019881.

Structurei

3D structure databases

ProteinModelPortaliQ5ZID6.
SMRiQ5ZID6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni198 – 199Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the pyrimidine 5'-nucleotidase family.Curated

Phylogenomic databases

eggNOGiKOG3128. Eukaryota.
ENOG410ZQJ8. LUCA.
GeneTreeiENSGT00390000012959.
HOGENOMiHOG000244931.
HOVERGENiHBG059750.
InParanoidiQ5ZID6.
KOiK01081.
OMAiHNVIDNC.
OrthoDBiEOG091G0BCN.
PhylomeDBiQ5ZID6.
TreeFamiTF314663.

Family and domain databases

CDDicd07504. HAD_5NT. 1 hit.
Gene3Di3.40.50.1000. 1 hit.
InterProiView protein in InterPro
IPR036412. HAD-like_sf.
IPR023214. HAD_sf.
IPR006434. Pyrimidine_nucleotidase_eu.
PfamiView protein in Pfam
PF05822. UMPH-1. 1 hit.
SFLDiSFLDG01128. C1.4:_5'-Nucleotidase_Like. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01544. HAD-SF-IE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 2 (identifier: Q5ZID6-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDRAAVAKMG AVASASVCAL VGGVVLAQYI FTMKKKTGRK TKIIEMMPEF
60 70 80 90 100
QKKTVHIKDP GRVEEIICGL IKGGAAKLQI ITDFDMTLSR FSYNGKRCPT
110 120 130 140 150
CHNIIDNSKL ITEECRKKLL QLKETYYAIE IDPALTIEEK YPYMVEWYNK
160 170 180 190 200
SHALLIEQGL QKDKLAEVVR ESDVMLKEGY ENFFDKLSEH NIPVFIFSAG
210 220 230 240 250
IGDILEEVIH QAGVYHSNVK VVSNFMDFDE NGILKGFKGE LIHVYNKHDG
260 270 280 290 300
ALKNTEYFKQ LKDNSNIILL GDSQGDLSMA DGVANVEHIL KIGYLNDKVD
310 320 330
ELLEKYMDSY DIVLVKDESL EVANSILQKI L
Length:331
Mass (Da):37,370
Last modified:November 14, 2006 - v3
Checksum:i7AE5CFE0F280224A
GO
Isoform 1 (identifier: Q5ZID6-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Show »
Length:285
Mass (Da):32,543
Checksum:iE56AFE6799F33A8A
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0215641 – 46Missing in isoform 1. 2 PublicationsAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF548635 mRNA. Translation: AAQ12342.1.
AJ496195 mRNA. Translation: CAD42712.1.
AJ720848 mRNA. Translation: CAG32507.1.
RefSeqiNP_989767.1. NM_204436.2. [Q5ZID6-1]
XP_015136753.1. XM_015281267.1. [Q5ZID6-3]
XP_015136759.1. XM_015281273.1. [Q5ZID6-1]
UniGeneiGga.5031.

Genome annotation databases

EnsembliENSGALT00000062778; ENSGALP00000057361; ENSGALG00000031397. [Q5ZID6-1]
ENSGALT00000078633; ENSGALP00000054083; ENSGALG00000031397. [Q5ZID6-3]
GeneIDi395080.
KEGGigga:395080.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry namei5NT3A_CHICK
AccessioniPrimary (citable) accession number: Q5ZID6
Secondary accession number(s): Q802T3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 14, 2006
Last modified: October 25, 2017
This is version 95 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families