Reviewed, UniProtKB/Swiss-Prot Q5ZID6 (5NT3_CHICK)

Names and origin

Protein names

Recommended name:
    Cytosolic 5'-nucleotidase III
      Short name=cN-III
    EC=3.1.3.5
Alternative name(s):
    Pyrimidine 5'-nucleotidase 1
      Short name=P5'N-1
      Short name=P5N-1
      Short name=PN-I

Gene names

Name:	NT5C3
Synonyms:	PYN1
ORF Names:	RCJMB04_27l2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Can act both as nucleotidase and as phosphotransferase By similarity.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Subcellular location	Cytoplasm Potential.
Developmental stage	Expressed at lower until E13 and the expression rises transiently between E13 and E16. Ref.1
Induction	By norepinephrine/NE and adenosine which are released by the embryo under hyposic conditions. By stimulation of beta-adrenergic/adenosine receptors in definitive red blood cells (RBC). Ref.1
Sequence similarities	Belongs to the pyrimidine 5'-nucleotidase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	5'-nucleotidase activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q5ZID6-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: Q5ZID6-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 331	331	Cytosolic 5'-nucleotidase III		PRO_0000064386
Regions
	Region	198 – 199	2	Substrate binding By similarity
Sites
	Active site	83	1	Nucleophile By similarity
	Active site	85	1	Proton donor By similarity
	Metal binding	83	1	Magnesium By similarity
	Metal binding	85	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	272	1	Magnesium By similarity
	Binding site	247	1	Substrate By similarity
Natural variations
	Alternative sequence	1 – 46	46	Missing in isoform 1.		VSP_021564

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified November 14, 2006. Version 3. Checksum: 7AE5CFE0F280224A	FASTA	331	37,370
	10 20 30 40 50 60 MDRAAVAKMG AVASASVCAL VGGVVLAQYI FTMKKKTGRK TKIIEMMPEF QKKTVHIKDP 70 80 90 100 110 120 GRVEEIICGL IKGGAAKLQI ITDFDMTLSR FSYNGKRCPT CHNIIDNSKL ITEECRKKLL 130 140 150 160 170 180 QLKETYYAIE IDPALTIEEK YPYMVEWYNK SHALLIEQGL QKDKLAEVVR ESDVMLKEGY 190 200 210 220 230 240 ENFFDKLSEH NIPVFIFSAG IGDILEEVIH QAGVYHSNVK VVSNFMDFDE NGILKGFKGE 250 260 270 280 290 300 LIHVYNKHDG ALKNTEYFKQ LKDNSNIILL GDSQGDLSMA DGVANVEHIL KIGYLNDKVD 310 320 330 ELLEKYMDSY DIVLVKDESL EVANSILQKI L
	Isoform 1. Checksum: E56AFE6799F33A8A	FASTA	285	32,543
	10 20 30 40 50 60 MPEFQKKTVH IKDPGRVEEI ICGLIKGGAA KLQIITDFDM TLSRFSYNGK RCPTCHNIID 70 80 90 100 110 120 NSKLITEECR KKLLQLKETY YAIEIDPALT IEEKYPYMVE WYNKSHALLI EQGLQKDKLA 130 140 150 160 170 180 EVVRESDVML KEGYENFFDK LSEHNIPVFI FSAGIGDILE EVIHQAGVYH SNVKVVSNFM 190 200 210 220 230 240 DFDENGILKG FKGELIHVYN KHDGALKNTE YFKQLKDNSN IILLGDSQGD LSMADGVANV 250 260 270 280 EHILKIGYLN DKVDELLEKY MDSYDIVLVK DESLEVANSI LQKIL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Erythroid pyrimidine 5'-nucleotidase: cloning, developmental expression, and regulation by cAMP and in vivo hypoxia." Mass M., Simo E., Dragon S. Blood 102:4198-4205(2003) [PubMed: 12907448] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 46-85; 150-158; 220-243 AND 291-290, DEVELOPMENTAL STAGE, INDUCTION.
[2]	"Cloning and gene expression of pyrimidine 5'-nucleotidase in chick embryonic erythrocytes." Mass M., Dragon S. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Blood.
[3]	"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF548635 mRNA. Translation: AAQ12342.1. AJ496195 mRNA. Translation: CAD42712.1. AJ720848 mRNA. Translation: CAG32507.1.
IPI	IPI00579830. IPI00651313.
RefSeq	NP_989767.1.
UniGene	Gga.5031
3D structure databases
SMR	Q5ZID6. Positions 44-331.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZID6.
Genome annotation databases
Ensembl	ENSGALT00000019908; ENSGALP00000019881; ENSGALG00000012192; Gallus gallus. [Genome view] ENSGALT00000037476; ENSGALP00000036684; ENSGALG00000012192; Gallus gallus. [Genome view]
GeneID	395080.
KEGG	gga:395080.
Organism-specific databases
CTD	395080.
Phylogenomic databases
eggNOG	veNOG13465.
HOGENOM	HBG382723.
HOVERGEN	Q5ZID6.
InParanoid	Q5ZID6.
OMA	YYAIEID.
OrthoDB	EOG9B5RRH.
PhylomeDB	Q5ZID6.
Enzyme and pathway databases
BRENDA	3.1.3.5. 4.
Family and domain databases
InterPro	IPR006434. HAD-SF_hydro_IE. IPR018012. HAD-SF_hydro_IE_pln. [Graphical view]
PANTHER	PTHR13045. HAD-SF_hydro_IE. 1 hit.
TIGRFAMs	TIGR01544. HAD-SF-IE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

5NT3_CHICK

Accession

Primary (citable) accession number: Q5ZID6
Secondary accession number(s): Q802T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	November 14, 2006
Last modified:	February 9, 2010
This is version 42 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)