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Q5ZID6 (5NT3_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytosolic 5'-nucleotidase III
Short name=cN-III
EC=3.1.3.5
Alternative name(s):
Pyrimidine 5'-nucleotidase 1
Short name=P5'N-1
Short name=P5N-1
Short name=PN-I
Gene names
Name:NT5C3
Synonyms:PYN1
ORF Names:RCJMB04_27l2
OrganismGallus gallus (Chicken)
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can act both as nucleotidase and as phosphotransferase By similarity.

Catalytic activity

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Subcellular location

Cytoplasm Potential.

Developmental stage

Expressed at lower until E13 and the expression rises transiently between E13 and E16. Ref.1

Induction

By norepinephrine/NE and adenosine which are released by the embryo under hyposic conditions. By stimulation of beta-adrenergic/adenosine receptors in definitive red blood cells (RBC). Ref.1

Sequence similarities

Belongs to the pyrimidine 5'-nucleotidase family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: Q5ZID6-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: Q5ZID6-1)

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 331331Cytosolic 5'-nucleotidase III
PRO_0000064386

Regions

Region198 – 1992Substrate binding By similarity

Sites

Active site831Nucleophile By similarity
Active site851Proton donor By similarity
Metal binding831Magnesium By similarity
Metal binding851Magnesium; via carbonyl oxygen By similarity
Metal binding2721Magnesium By similarity
Binding site2471Substrate By similarity

Natural variations

Alternative sequence1 – 4646Missing in isoform 1.
VSP_021564

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 [UniParc].

Last modified November 14, 2006. Version 3.
Checksum: 7AE5CFE0F280224A

FASTA33137,370
        10         20         30         40         50         60 
MDRAAVAKMG AVASASVCAL VGGVVLAQYI FTMKKKTGRK TKIIEMMPEF QKKTVHIKDP 

        70         80         90        100        110        120 
GRVEEIICGL IKGGAAKLQI ITDFDMTLSR FSYNGKRCPT CHNIIDNSKL ITEECRKKLL 

       130        140        150        160        170        180 
QLKETYYAIE IDPALTIEEK YPYMVEWYNK SHALLIEQGL QKDKLAEVVR ESDVMLKEGY 

       190        200        210        220        230        240 
ENFFDKLSEH NIPVFIFSAG IGDILEEVIH QAGVYHSNVK VVSNFMDFDE NGILKGFKGE 

       250        260        270        280        290        300 
LIHVYNKHDG ALKNTEYFKQ LKDNSNIILL GDSQGDLSMA DGVANVEHIL KIGYLNDKVD 

       310        320        330 
ELLEKYMDSY DIVLVKDESL EVANSILQKI L

« Hide

Isoform 1 [UniParc].

Checksum: E56AFE6799F33A8A
Show »

FASTA28532,543

References

« Hide 'large scale' references
[1]"Erythroid pyrimidine 5'-nucleotidase: cloning, developmental expression, and regulation by cAMP and in vivo hypoxia."
Mass M., Simo E., Dragon S.
Blood 102:4198-4205(2003) [PubMed: 12907448] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 46-85; 150-158; 220-243 AND 291-290, DEVELOPMENTAL STAGE, INDUCTION.
[2]"Cloning and gene expression of pyrimidine 5'-nucleotidase in chick embryonic erythrocytes."
Mass M., Dragon S.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Blood.
[3]"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis."
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.
Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: CB.
Tissue: Bursa of Fabricius.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF548635 mRNA. Translation: AAQ12342.1.
AJ496195 mRNA. Translation: CAD42712.1.
AJ720848 mRNA. Translation: CAG32507.1.
IPIIPI00579830.
IPI00651313.
RefSeqNP_989767.1. NM_204436.2.
UniGeneGga.5031.

3D structure databases

ProteinModelPortalQ5ZID6.
SMRQ5ZID6. Positions 44-331.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ5ZID6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSGALT00000019908; ENSGALP00000019881; ENSGALG00000012192.
ENSGALT00000037476; ENSGALP00000036684; ENSGALG00000012192.
GeneID395080.
KEGGgga:395080.

Organism-specific databases

CTD51251.

Phylogenomic databases

eggNOGveNOG13465.
GeneTreeENSGT00390000012959.
HOGENOMHBG382723.
HOVERGENHBG059750.
InParanoidQ5ZID6.
OMANTEYFKQ.
OrthoDBEOG4JWVDW.

Family and domain databases

InterProIPR023214. HAD-like_dom.
IPR006434. Pyrimidine_nucleotidase_eu.
[Graphical view]
Gene3DG3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
KOK01081.
PANTHERPTHR13045. HAD-SF_hydro_IE. 1 hit.
PfamPF05822. UMPH-1. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01544. HAD-SF-IE. 1 hit.
ProtoNetSearch...

Entry information

Entry name5NT3_CHICK
AccessionPrimary (citable) accession number: Q5ZID6
Secondary accession number(s): Q802T3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 14, 2006
Last modified: December 14, 2011
This is version 55 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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