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Q5ZID5

- UBP28_CHICK

UniProt

Q5ZID5 - UBP28_CHICK

Protein

Ubiquitin carboxyl-terminal hydrolase 28

Gene

USP28

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei165 – 1651NucleophilePROSITE-ProRule annotation
    Active sitei601 – 6011Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB
    2. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. cell proliferation Source: UniProtKB
    2. DNA damage checkpoint Source: UniProtKB
    3. DNA repair Source: UniProtKB-KW
    4. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
    5. protein deubiquitination Source: UniProtKB
    6. response to ionizing radiation Source: UniProtKB
    7. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    DNA damage, DNA repair, Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.054.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 28 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 28
    Ubiquitin thioesterase 28
    Ubiquitin-specific-processing protease 28
    Gene namesi
    Name:USP28
    ORF Names:RCJMB04_27l24
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Nucleusnucleoplasm By similarity

    GO - Cellular componenti

    1. nucleoplasm Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10471047Ubiquitin carboxyl-terminal hydrolase 28PRO_0000080659Add
    BLAST

    Proteomic databases

    PaxDbiQ5ZID5.

    Interactioni

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000011273.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5ZID5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati94 – 11320UIMAdd
    BLAST
    Domaini156 – 651496USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP28 subfamily.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    HOGENOMiHOG000007956.
    HOVERGENiHBG056030.
    InParanoidiQ5ZID5.
    KOiK11849.
    PhylomeDBiQ5ZID5.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR009060. UBA-like.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    SUPFAMiSSF46934. SSF46934. 1 hit.
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5ZID5-1 [UniParc]FASTAAdd to Basket

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    MTAELQAASG GPGGLEADCQ VLNKMKEITG IQDADFLHAA LKAAKGNLME     50
    ALIVLTEERD QEPVQNTAAA EPSSWEGSAV GKEPPQGGAA FDPEKKGDVH 100
    SAVAYGQLES PKAHAAERPQ EVHSPEHKNR SKRKRCEVWG ENSKQSDWKR 150
    VGDWPVGMKN IGNTCWFSAV IQSLFQLPQF RRLVLSYSFP PNVLESCRTR 200
    TGKRNIAFMQ ELQCLFALML GTRRKFVDPS AALELLRDAF KSTEEQQQDV 250
    SEFTHKLLDW LEDAFQLAVN VRSPGDKSEN PMVQLFYGTF LTEGVHEGNT 300
    FSKIETFGQY PLQVNGYRNL NECLEGAMVE GEMDEETATQ SVKYVQERWF 350
    TKLPPVLTFE LSRFEFNQSL GQPEKIHTKL EFPQTIFMDR YLYCSKELIQ 400
    TKREEMKKLK EKMLVLQQKL ERYMKYGSGP ARFPLPDMLQ YVLEFITTKP 450
    AGAVSSACVS STEDSQMMDR QSQGESLILG TPSQPDSMLD GKDGKPEDEA 500
    VLLANSSPQQ QLNAPLQPSE PPAEMSDCPA PHVVSEEEMN LVTTCLQRWR 550
    NEIEQDVRDL KESIARVSLS IDEMYSDPHL QQVPYHLHAV LVHEGQANAG 600
    HYWAFIYDQP RKSWLKYNDI SVTESSWEEL ERDSFGGLRN ASAYCLMYIS 650
    DKVSHVVAGE GDGSEVGQFQ KEVEALPPEL RRYIQEDNWR LEQEAEEWEE 700
    EQSCKIPSTA SESQELSPES GLDPPAAHEQ SLRSLSSEHA MIAKEQTAKA 750
    IANAANVYEK NGVEAALCEA FHEEYSRLYL LSKETPTPQN DARLQHVLVY 800
    FLQNDAPQQI VERTLLEQFA DKNLSYDERS ISIMKVARDK LKEIGPDEVN 850
    MEEYKKWHED YSLFRKVSVY LLTGLELYQN RKYKESLTYL IYAYQSNTTL 900
    LKKGANRGVN ESLITLYRRK CLLKLNEVAS SLFVSCEEAH VAEGISILNE 950
    LIIPCMHLIN NFDISREDMD AIEVMRNRWC SYLGREDMDA SLQIRLGELL 1000
    PRLLDGSTEV VVLKEPPKIR PNSPYDLCSR FAAVMESIHD ASTVTVK 1047
    Length:1,047
    Mass (Da):118,747
    Last modified:November 23, 2004 - v1
    Checksum:i93B40127861B2A00
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720849 mRNA. Translation: CAG32508.1.
    RefSeqiNP_001026667.1. NM_001031496.1.
    UniGeneiGga.22397.

    Genome annotation databases

    GeneIDi428246.
    KEGGigga:428246.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720849 mRNA. Translation: CAG32508.1 .
    RefSeqi NP_001026667.1. NM_001031496.1.
    UniGenei Gga.22397.

    3D structure databases

    ProteinModelPortali Q5ZID5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000011273.

    Protein family/group databases

    MEROPSi C19.054.

    Proteomic databases

    PaxDbi Q5ZID5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 428246.
    KEGGi gga:428246.

    Organism-specific databases

    CTDi 57646.

    Phylogenomic databases

    eggNOGi COG5077.
    HOGENOMi HOG000007956.
    HOVERGENi HBG056030.
    InParanoidi Q5ZID5.
    KOi K11849.
    PhylomeDBi Q5ZID5.

    Miscellaneous databases

    NextBioi 20829288.
    PROi Q5ZID5.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR009060. UBA-like.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46934. SSF46934. 1 hit.
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: CB.
      Tissue: Bursa of Fabricius.

    Entry informationi

    Entry nameiUBP28_CHICK
    AccessioniPrimary (citable) accession number: Q5ZID5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3