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Protein

Ubiquitin carboxyl-terminal hydrolase 28

Gene

USP28

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei165 – 1651NucleophilePROSITE-ProRule annotation
Active sitei601 – 6011Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. cell proliferation Source: UniProtKB
  2. DNA damage checkpoint Source: UniProtKB
  3. DNA repair Source: UniProtKB-KW
  4. intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: UniProtKB
  5. protein deubiquitination Source: UniProtKB
  6. response to ionizing radiation Source: UniProtKB
  7. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.054.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 28 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 28
Ubiquitin thioesterase 28
Ubiquitin-specific-processing protease 28
Gene namesi
Name:USP28
ORF Names:RCJMB04_27l24
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Nucleusnucleoplasm By similarity

GO - Cellular componenti

  1. nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10471047Ubiquitin carboxyl-terminal hydrolase 28PRO_0000080659Add
BLAST

Proteomic databases

PaxDbiQ5ZID5.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000011273.

Structurei

3D structure databases

ProteinModelPortaliQ5ZID5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 11320UIMCuratedAdd
BLAST
Domaini156 – 651496USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. USP28 subfamily.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000007956.
HOVERGENiHBG056030.
InParanoidiQ5ZID5.
KOiK11849.
PhylomeDBiQ5ZID5.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZID5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAELQAASG GPGGLEADCQ VLNKMKEITG IQDADFLHAA LKAAKGNLME
60 70 80 90 100
ALIVLTEERD QEPVQNTAAA EPSSWEGSAV GKEPPQGGAA FDPEKKGDVH
110 120 130 140 150
SAVAYGQLES PKAHAAERPQ EVHSPEHKNR SKRKRCEVWG ENSKQSDWKR
160 170 180 190 200
VGDWPVGMKN IGNTCWFSAV IQSLFQLPQF RRLVLSYSFP PNVLESCRTR
210 220 230 240 250
TGKRNIAFMQ ELQCLFALML GTRRKFVDPS AALELLRDAF KSTEEQQQDV
260 270 280 290 300
SEFTHKLLDW LEDAFQLAVN VRSPGDKSEN PMVQLFYGTF LTEGVHEGNT
310 320 330 340 350
FSKIETFGQY PLQVNGYRNL NECLEGAMVE GEMDEETATQ SVKYVQERWF
360 370 380 390 400
TKLPPVLTFE LSRFEFNQSL GQPEKIHTKL EFPQTIFMDR YLYCSKELIQ
410 420 430 440 450
TKREEMKKLK EKMLVLQQKL ERYMKYGSGP ARFPLPDMLQ YVLEFITTKP
460 470 480 490 500
AGAVSSACVS STEDSQMMDR QSQGESLILG TPSQPDSMLD GKDGKPEDEA
510 520 530 540 550
VLLANSSPQQ QLNAPLQPSE PPAEMSDCPA PHVVSEEEMN LVTTCLQRWR
560 570 580 590 600
NEIEQDVRDL KESIARVSLS IDEMYSDPHL QQVPYHLHAV LVHEGQANAG
610 620 630 640 650
HYWAFIYDQP RKSWLKYNDI SVTESSWEEL ERDSFGGLRN ASAYCLMYIS
660 670 680 690 700
DKVSHVVAGE GDGSEVGQFQ KEVEALPPEL RRYIQEDNWR LEQEAEEWEE
710 720 730 740 750
EQSCKIPSTA SESQELSPES GLDPPAAHEQ SLRSLSSEHA MIAKEQTAKA
760 770 780 790 800
IANAANVYEK NGVEAALCEA FHEEYSRLYL LSKETPTPQN DARLQHVLVY
810 820 830 840 850
FLQNDAPQQI VERTLLEQFA DKNLSYDERS ISIMKVARDK LKEIGPDEVN
860 870 880 890 900
MEEYKKWHED YSLFRKVSVY LLTGLELYQN RKYKESLTYL IYAYQSNTTL
910 920 930 940 950
LKKGANRGVN ESLITLYRRK CLLKLNEVAS SLFVSCEEAH VAEGISILNE
960 970 980 990 1000
LIIPCMHLIN NFDISREDMD AIEVMRNRWC SYLGREDMDA SLQIRLGELL
1010 1020 1030 1040
PRLLDGSTEV VVLKEPPKIR PNSPYDLCSR FAAVMESIHD ASTVTVK
Length:1,047
Mass (Da):118,747
Last modified:November 23, 2004 - v1
Checksum:i93B40127861B2A00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720849 mRNA. Translation: CAG32508.1.
RefSeqiNP_001026667.1. NM_001031496.1.
UniGeneiGga.22397.

Genome annotation databases

GeneIDi428246.
KEGGigga:428246.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720849 mRNA. Translation: CAG32508.1.
RefSeqiNP_001026667.1. NM_001031496.1.
UniGeneiGga.22397.

3D structure databases

ProteinModelPortaliQ5ZID5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000011273.

Protein family/group databases

MEROPSiC19.054.

Proteomic databases

PaxDbiQ5ZID5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi428246.
KEGGigga:428246.

Organism-specific databases

CTDi57646.

Phylogenomic databases

eggNOGiCOG5077.
HOGENOMiHOG000007956.
HOVERGENiHBG056030.
InParanoidiQ5ZID5.
KOiK11849.
PhylomeDBiQ5ZID5.

Miscellaneous databases

NextBioi20829288.
PROiQ5ZID5.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR009060. UBA-like.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.

Entry informationi

Entry nameiUBP28_CHICK
AccessioniPrimary (citable) accession number: Q5ZID5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: November 23, 2004
Last modified: March 4, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.